FastQCFastQC Report
Thu 2 Feb 2017
SRR4062367_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062367_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605030
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21760.13557378989800814No Hit
GCGCAAGACGGACCAGAGCGAAAGC18090.11270817367899666No Hit
GAATAGGACCGCGGTTCTATTTTGT17920.11164900344541846No Hit
GATTAAGAGGGACGGCCGGGGGCAT17800.11090135386877503No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17310.10784845143081438No Hit
GAATAACGCCGCCGCATCGCCAGTC16930.10548089443811019No Hit
GTCTTGCGCCGGTCCAAGAATTTCA16880.10516937378117543No Hit
GTATCTGATCGTCTTCGAACCTCCG16850.10498246138701457No Hit
GAACTACGACGGTATCTGATCGTCT16440.10242799200014953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC852.712659E-713.4062863
TAGGACG957.411654E-812.9967164
CGTCTTA1353.7107384E-1011.96478915
GTTATAT1701.8189894E-1211.7279411
CAATACT907.48188E-611.6081774
TCCAATA3200.011.5715912
TACGCTA3000.011.4006749
CGGTCCA3000.011.40067410
CGCGTAA1251.8299033E-811.40067410
CGAACGA1604.5474735E-1111.28473216
CGTTATT1354.76939E-911.2529062
CCGTCGT2900.011.138599
CGTCGTA2900.011.1385910
AGGATAG600.005880033611.082265
CGGTTCT3800.011.00064912
AAGACCG951.36161925E-510.9989365
TCGCGTA1303.2625394E-810.9621879
CGACTTT1502.1300366E-910.767302513
AGCTTAA1158.8085835E-710.73976610
ACGCTAT3100.010.7264410
Produced by FastQC (version 0.11.5)