##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062367_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1605030
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.952463193834383	32.0	32.0	32.0	32.0	32.0
2	30.438804882151736	32.0	32.0	32.0	32.0	32.0
3	30.51710123798309	32.0	32.0	32.0	32.0	32.0
4	30.281034622405812	32.0	32.0	32.0	21.0	32.0
5	30.344173006112037	32.0	32.0	32.0	21.0	32.0
6	33.614454558481775	36.0	36.0	36.0	21.0	36.0
7	33.62151548569186	36.0	36.0	36.0	27.0	36.0
8	33.54419792776459	36.0	36.0	36.0	21.0	36.0
9	33.76763985719893	36.0	36.0	36.0	32.0	36.0
10	33.28104459106683	36.0	36.0	36.0	21.0	36.0
11	33.86941427886083	36.0	36.0	36.0	32.0	36.0
12	33.50735188750366	36.0	36.0	36.0	27.0	36.0
13	33.71498788184645	36.0	36.0	36.0	32.0	36.0
14	33.504971246643365	36.0	36.0	36.0	27.0	36.0
15	33.40321863142745	36.0	36.0	36.0	27.0	36.0
16	33.43554575303889	36.0	36.0	36.0	27.0	36.0
17	33.334115250182236	36.0	36.0	36.0	21.0	36.0
18	33.38485075045326	36.0	36.0	36.0	27.0	36.0
19	33.336740746278885	36.0	36.0	36.0	21.0	36.0
20	33.26933266044871	36.0	36.0	36.0	21.0	36.0
21	33.25148688809555	36.0	36.0	36.0	21.0	36.0
22	33.198659838133864	36.0	36.0	36.0	21.0	36.0
23	33.18573422303633	36.0	36.0	36.0	21.0	36.0
24	33.164924643153086	36.0	36.0	36.0	21.0	36.0
25	32.382683812763624	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	11.0
4	53.0
5	150.0
6	275.0
7	65.0
8	237.0
9	281.0
10	173.0
11	69.0
12	110.0
13	91.0
14	494.0
15	1029.0
16	1459.0
17	1920.0
18	2362.0
19	3005.0
20	3928.0
21	4821.0
22	6442.0
23	9029.0
24	11822.0
25	16422.0
26	22425.0
27	30474.0
28	41938.0
29	58126.0
30	78776.0
31	113904.0
32	169768.0
33	239877.0
34	422933.0
35	362560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.46981809481202	17.773483988430446	11.250842730584171	26.50585518617336
2	17.30101952124932	19.9419428038797	36.184997965692794	26.572039709178185
3	18.761277539627155	23.527377629349015	28.427562057621376	29.28378277340245
4	12.911036986863891	15.352505245159403	35.61692234546954	36.11953542250717
5	15.009644899421929	35.92577011171878	33.39216877794917	15.67241621091011
6	34.295644759043995	35.02605814260078	16.815651964692325	13.862645133662907
7	30.328555664312123	30.248380600876562	20.89040455364435	18.532659181166967
8	28.290123518378095	32.07150197549587	19.689482978808318	19.948891527317716
9	27.401540233598148	14.236403219660708	18.810643813652813	39.551412733088334
10	16.210363090526563	26.120362348265257	31.1736648184984	26.49560974270978
11	37.48655783188744	21.040143116986158	22.11969580155243	19.35360324957397
12	24.534377898928422	23.68788300166629	28.32454648048645	23.453192618918838
13	29.208618374146123	19.172520308670585	25.289542414759097	26.32931890242419
14	23.6464371684982	19.613803324889385	24.55291896244887	32.186840544163545
15	25.40112622221557	26.896102632425915	21.962144063631307	25.740627081727208
16	26.22901024273958	25.45659466020326	23.24444483253726	25.069950264519903
17	24.365497749058544	25.445650227963572	24.973130302593653	25.215721720384227
18	25.28187667419835	24.390987931423837	25.783995817857203	24.543139576520613
19	25.715146622681317	24.97046086048418	24.94993630525566	24.364456211578844
20	25.748001731603892	24.096178869192798	24.535005720031588	25.62081367917172
21	26.949649811321695	24.004970701681167	24.046206065778204	24.99917342121893
22	25.99991638895111	24.133580505896763	24.453423966046426	25.413079139105697
23	24.589258775394082	23.9349801663803	25.183893029471548	26.291868028754067
24	24.84656146712067	24.925167912100527	24.68098884412342	25.547281776655385
25	24.79459746093141	24.441623257119684	24.91462615802115	25.84915312392776
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	126.0
1	126.0
2	382.0
3	638.0
4	638.0
5	638.0
6	1521.0
7	2404.0
8	2404.0
9	2404.0
10	2657.0
11	2910.0
12	2910.0
13	2910.0
14	3895.5
15	4881.0
16	4881.0
17	4881.0
18	7762.5
19	10644.0
20	10644.0
21	10644.0
22	17059.5
23	23475.0
24	23475.0
25	23475.0
26	34527.0
27	45579.0
28	45579.0
29	45579.0
30	55270.0
31	64961.0
32	64961.0
33	64961.0
34	82056.0
35	99151.0
36	99151.0
37	99151.0
38	112654.5
39	126158.0
40	126158.0
41	126158.0
42	143814.0
43	161470.0
44	161470.0
45	161470.0
46	183144.0
47	204818.0
48	204818.0
49	204818.0
50	208114.5
51	211411.0
52	211411.0
53	211411.0
54	197793.5
55	184176.0
56	184176.0
57	184176.0
58	170113.5
59	156051.0
60	156051.0
61	156051.0
62	139880.0
63	123709.0
64	123709.0
65	123709.0
66	103887.5
67	84066.0
68	84066.0
69	84066.0
70	64519.0
71	44972.0
72	44972.0
73	44972.0
74	35317.5
75	25663.0
76	25663.0
77	25663.0
78	21040.5
79	16418.0
80	16418.0
81	16418.0
82	11626.5
83	6835.0
84	6835.0
85	6835.0
86	4903.5
87	2972.0
88	2972.0
89	2972.0
90	1916.5
91	861.0
92	861.0
93	861.0
94	484.5
95	108.0
96	108.0
97	108.0
98	340.5
99	573.0
100	573.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00797492881752989
2	0.0038005520146040883
3	0.004361289197086659
4	0.013519996510968642
5	0.03457879291975851
6	0.04566892830663601
7	0.06479629664243036
8	0.08610430957676804
9	0.09389232600013707
10	0.11345582325564008
11	0.11625950916805293
12	0.12878263957683034
13	0.11819093724104845
14	0.11893858681769189
15	0.11177361170819236
16	0.10890762166439256
17	0.11981084465710921
18	0.12610356192719138
19	0.1290941602337651
20	0.1178794165841137
21	0.12691351563522177
22	0.14772309551846383
23	0.13681987252574718
24	0.13133710896369538
25	0.12959259328486072
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1605030.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.294203096832746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.54974258856534	43.168730045177
2	10.360707688572376	10.83609904186241
3	2.79825646478896	4.389977756600973
4	1.1959801095558982	2.501713069955537
5	0.6789373545312261	1.7752243953941138
6	0.43942553228671566	1.3787644818801206
7	0.30514528850825623	1.1170130783904666
8	0.223680337015	0.9357747978104326
9	0.18505789648293783	0.8709709701016262
>10	1.0343427496551334	10.06340859708876
>50	0.10854981883061697	3.993219820553975
>100	0.103347521276077	11.548245838852734
>500	0.012888497819814973	4.554762207790997
>1k	0.003938152111610131	2.8660958985409235
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2176	0.13557378989800814	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1809	0.11270817367899666	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1792	0.11164900344541846	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1780	0.11090135386877503	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1731	0.10784845143081438	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1693	0.10548089443811019	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1688	0.10516937378117543	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1685	0.10498246138701457	No Hit
GAACTACGACGGTATCTGATCGTCT	1644	0.10242799200014953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.869123941608568E-4
2	0.0	0.0	0.0	0.0	1.869123941608568E-4
3	0.0	0.0	0.0	0.0	2.4921652554780907E-4
4	0.0	0.0	0.0	0.0	2.4921652554780907E-4
5	0.0	0.0	0.0	0.0	2.4921652554780907E-4
6	0.0	0.0	0.0	0.0	3.1152065693476134E-4
7	0.0	0.0	0.0	0.0	3.1152065693476134E-4
8	0.0	0.0	0.0	0.0	3.1152065693476134E-4
9	0.0	0.0	0.0	1.2460826277390453E-4	3.1152065693476134E-4
10	0.0	0.0	0.0	1.869123941608568E-4	3.1152065693476134E-4
11	0.0	0.0	0.0	1.869123941608568E-4	3.1152065693476134E-4
12	0.0	0.0	0.0	1.869123941608568E-4	3.1152065693476134E-4
13	0.0	0.0	0.0	1.869123941608568E-4	3.1152065693476134E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	85	2.712659E-7	13.406286	3
TAGGACG	95	7.411654E-8	12.996716	4
CGTCTTA	135	3.7107384E-10	11.964789	15
GTTATAT	170	1.8189894E-12	11.727941	1
CAATACT	90	7.48188E-6	11.608177	4
TCCAATA	320	0.0	11.571591	2
TACGCTA	300	0.0	11.400674	9
CGGTCCA	300	0.0	11.400674	10
CGCGTAA	125	1.8299033E-8	11.400674	10
CGAACGA	160	4.5474735E-11	11.284732	16
CGTTATT	135	4.76939E-9	11.252906	2
CCGTCGT	290	0.0	11.13859	9
CGTCGTA	290	0.0	11.13859	10
AGGATAG	60	0.0058800336	11.08226	5
CGGTTCT	380	0.0	11.000649	12
AAGACCG	95	1.36161925E-5	10.998936	5
TCGCGTA	130	3.2625394E-8	10.962187	9
CGACTTT	150	2.1300366E-9	10.7673025	13
AGCTTAA	115	8.8085835E-7	10.739766	10
ACGCTAT	310	0.0	10.72644	10
>>END_MODULE