FastQCFastQC Report
Thu 2 Feb 2017
SRR4062367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605030
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18550.11557416372279647No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC18220.11351812738702703No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG17310.10784845143081438No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16170.10074578045270181No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16090.1002473474016062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG1259.968062E-1021.1199865
GGACCGT1158.776624E-921.0434656
GTATCAA24200.021.0006391
AACGAAT550.0044842619.99998731
ATGCGTA750.00129112717.59998722
ATTTCGT750.00129112717.59998742
ACCGTCG4650.017.5053638
ATACCGT4600.017.217386
ATAGCGT4100.017.170726
TAGTACG2007.2759576E-1116.4999892
TACCGTC4800.016.0416567
GTATTAG5500.016.0004861
TAGCGTA4000.015.9499887
TAGACAG3200.015.8124885
AACGCAG31600.015.8037866
AATACTG2650.015.7735755
CGTATAT4050.015.75307610
CGCTTCG4200.015.71427532
GTTTAGA2550.015.5298851
TCAACGC32250.015.485264
Produced by FastQC (version 0.11.5)