##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062367_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1605030
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0825704192445	32.0	32.0	32.0	32.0	32.0
2	30.907473380559864	32.0	32.0	32.0	32.0	32.0
3	31.204021108639715	32.0	32.0	32.0	32.0	32.0
4	31.42951035183143	32.0	32.0	32.0	32.0	32.0
5	31.244306336953205	32.0	32.0	32.0	32.0	32.0
6	34.86211036553834	36.0	36.0	36.0	32.0	36.0
7	34.87737425468683	36.0	36.0	36.0	32.0	36.0
8	34.7921116739251	36.0	36.0	36.0	32.0	36.0
9	35.018670056011416	36.0	36.0	36.0	36.0	36.0
10	34.78029943365544	36.0	36.0	36.0	32.0	36.0
11	35.027458053743544	36.0	36.0	36.0	36.0	36.0
12	34.89199142695152	36.0	36.0	36.0	32.0	36.0
13	34.9772695837461	36.0	36.0	36.0	32.0	36.0
14	34.9185840763101	36.0	36.0	36.0	32.0	36.0
15	34.844256493648096	36.0	36.0	36.0	32.0	36.0
16	34.87018186575952	36.0	36.0	36.0	32.0	36.0
17	34.83607783032093	36.0	36.0	36.0	32.0	36.0
18	34.85449493155891	36.0	36.0	36.0	32.0	36.0
19	34.84656984604649	36.0	36.0	36.0	32.0	36.0
20	34.843007295813784	36.0	36.0	36.0	32.0	36.0
21	34.84625334105905	36.0	36.0	36.0	32.0	36.0
22	34.81444770502732	36.0	36.0	36.0	32.0	36.0
23	34.77122919820813	36.0	36.0	36.0	32.0	36.0
24	34.74207024167772	36.0	36.0	36.0	32.0	36.0
25	34.70972941315738	36.0	36.0	36.0	32.0	36.0
26	34.6582319333595	36.0	36.0	36.0	32.0	36.0
27	34.63819056341626	36.0	36.0	36.0	32.0	36.0
28	34.62565559522252	36.0	36.0	36.0	32.0	36.0
29	34.58976280817181	36.0	36.0	36.0	32.0	36.0
30	34.576211036553836	36.0	36.0	36.0	32.0	36.0
31	34.58462396341501	36.0	36.0	36.0	32.0	36.0
32	34.535984997165166	36.0	36.0	36.0	32.0	36.0
33	34.523438814227774	36.0	36.0	36.0	32.0	36.0
34	34.50606468414921	36.0	36.0	36.0	32.0	36.0
35	34.4931540220432	36.0	36.0	36.0	32.0	36.0
36	34.442202326436266	36.0	36.0	36.0	32.0	36.0
37	34.45554164096621	36.0	36.0	36.0	32.0	36.0
38	34.41078609122571	36.0	36.0	36.0	32.0	36.0
39	34.40131461717227	36.0	36.0	36.0	32.0	36.0
40	34.39783119318642	36.0	36.0	36.0	32.0	36.0
41	34.358628810676436	36.0	36.0	36.0	32.0	36.0
42	34.32701320224544	36.0	36.0	36.0	32.0	36.0
43	34.3271147579796	36.0	36.0	36.0	32.0	36.0
44	34.28305576842801	36.0	36.0	36.0	32.0	36.0
45	34.25008504513934	36.0	36.0	36.0	32.0	36.0
46	34.25614162975147	36.0	36.0	36.0	32.0	36.0
47	34.24210139374342	36.0	36.0	36.0	32.0	36.0
48	34.19874145654598	36.0	36.0	36.0	32.0	36.0
49	34.18469685924874	36.0	36.0	36.0	32.0	36.0
50	33.55205385569117	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	3.0
20	3.0
21	46.0
22	118.0
23	359.0
24	1007.0
25	2394.0
26	4968.0
27	9734.0
28	16688.0
29	27389.0
30	41782.0
31	64618.0
32	101515.0
33	178004.0
34	375322.0
35	781077.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.6708764216335	18.496252595267826	11.962097250573887	26.870773732524782
2	16.838600380441115	20.429488485519126	35.6516814238691	27.080229710170663
3	18.39225979939228	23.729628796596685	28.879523844829635	28.998587559181395
4	12.968675332346018	15.868498326198468	36.02146752488584	35.14135881656967
5	15.350180370460365	35.76437823592082	32.97857360921603	15.906867784402785
6	34.688139202642695	35.50800079998655	16.2746171871252	13.52924281024556
7	30.483417755431365	30.27183292524127	20.718740459679882	18.526008859647483
8	28.44825330367657	32.24363407537554	19.309607920101183	19.998504700846713
9	27.550949203441682	13.948711239042261	18.47535560082989	40.024983956686164
10	16.144059612592912	26.07702036722055	31.17748577908201	26.601434241104528
11	37.91686136707725	20.779486987782157	22.005196164557674	19.29845548058292
12	24.661408197977607	23.516445175479586	28.198849865734598	23.62329676080821
13	29.452720509897013	19.019519884363532	25.15074484589073	26.377014759848727
14	23.752951658224458	19.35602449798446	24.491380223422617	32.399643620368465
15	25.507996735263518	26.808346261440597	21.772614842090178	25.91104216120571
16	26.451966629907233	25.347252076285177	23.09321321096802	25.10756808283957
17	24.597048030254886	25.39460321613926	24.77118807748142	25.237160676124432
18	25.596530905964375	24.268082216531777	25.56076833454826	24.574618542955584
19	25.933471648505012	24.869690909204188	24.88439468421151	24.31244275807929
20	26.046865167629264	23.893198257976486	24.3792327869261	25.680703787468147
21	27.097188214550506	24.004286524239422	23.88846314399108	25.010062117218997
22	26.230724659352163	24.10764907821038	24.287957234444217	25.373669027993245
23	24.832619951028953	23.721363463611272	25.03616754827013	26.40984903708965
24	25.0262611913796	24.779910655875593	24.5511298854227	25.642698267322107
25	25.165137100241118	24.177741226020697	24.883273209846546	25.773848463891643
26	24.986511155554723	24.788197105350054	25.176414148022154	25.048877591073065
27	25.94144657732254	24.311508196108484	24.240793006984294	25.50625221958468
28	24.875547497554564	24.181853298692236	25.379401008080848	25.563198195672353
29	24.956605172488988	24.590132271670935	25.016354834489075	25.436907721351005
30	25.053176576138764	24.611128763948336	25.220089344124407	25.115605315788493
31	25.461455549117463	24.426957751568505	24.265465443013525	25.846121256300503
32	25.238157542226624	24.42378024086777	24.283533641115742	26.05452857578986
33	25.001651059481755	23.990268094677358	24.85785312424067	26.150227721600217
34	25.504383095643075	24.16066989402067	25.206008610430956	25.128938399905298
35	26.25184575989234	24.036809280823412	24.79567360111649	24.91567135816776
36	25.056852519890594	24.522033856064994	24.707264038678407	25.713849585366006
37	26.12810975495785	24.31082285066323	24.31979464558295	25.24127274879597
38	25.043083306854076	24.095873597378244	25.03560681108764	25.825436284680038
39	25.4326087362853	24.23325420708647	24.39113287602101	25.943004180607215
40	25.84275683320561	24.199734584400293	25.046198513423423	24.911310068970675
41	24.699662934649197	24.612312542444688	25.30980729332162	25.378217229584493
42	25.990168408067138	24.681968561335303	24.764646143685788	24.56321688691177
43	25.244637171890865	23.60628773293957	25.138346323744727	26.010728771424834
44	24.915795966430533	24.137305844750564	24.91610748708747	26.030790701731434
45	25.034859161510997	24.534307769948224	24.916792832532725	25.51404023600805
46	24.812012742448893	24.17501490627272	25.146274615598845	25.866697735679544
47	24.96451779717513	23.98484763524669	25.520146040883972	25.530488526694207
48	25.6785231428696	24.742278960517872	24.535616156707352	25.043581739905175
49	24.577422228867995	24.754303657875553	25.058472427306654	25.609801685949797
50	24.552313663919055	24.86352280019688	24.8767935801823	25.707369955701765
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	15.5
2	18.0
3	61.0
4	104.0
5	107.0
6	110.0
7	148.0
8	186.0
9	244.0
10	302.0
11	368.5
12	435.0
13	856.5
14	1278.0
15	1647.0
16	2016.0
17	2471.0
18	2926.0
19	3300.0
20	3674.0
21	4581.0
22	5488.0
23	6046.0
24	6604.0
25	8551.0
26	10498.0
27	13212.0
28	15926.0
29	18276.0
30	20626.0
31	21712.0
32	22798.0
33	26178.5
34	29559.0
35	34731.5
36	39904.0
37	49113.0
38	58322.0
39	63116.0
40	67910.0
41	75218.5
42	82527.0
43	83504.0
44	84481.0
45	92791.0
46	101101.0
47	107913.0
48	114725.0
49	118259.5
50	121794.0
51	116987.5
52	112181.0
53	110671.5
54	109162.0
55	111319.5
56	113477.0
57	111920.0
58	110363.0
59	101372.5
60	92382.0
61	82321.0
62	72260.0
63	63986.5
64	55713.0
65	48087.5
66	40462.0
67	36511.5
68	32561.0
69	29833.0
70	27105.0
71	21358.5
72	15612.0
73	13732.0
74	11852.0
75	8911.5
76	5971.0
77	5288.5
78	4606.0
79	3892.0
80	3178.0
81	2553.5
82	1929.0
83	1652.0
84	1375.0
85	1128.0
86	881.0
87	636.5
88	392.0
89	275.5
90	159.0
91	101.5
92	44.0
93	31.0
94	18.0
95	15.5
96	13.0
97	10.0
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.011588568437973122
2	0.003177510700734566
3	4.3612891970866587E-4
4	6.230413138695227E-5
5	0.0
6	1.869123941608568E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	6.230413138695227E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1605030.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.10385719583093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.56956739775268	46.885750752008846
2	9.999730071824306	11.220468558929742
3	2.5662159653289436	4.319238421574294
4	1.0635245279894554	2.386713129703356
5	0.6178679517973682	1.7332387666760047
6	0.42271944172499976	1.4229714715464459
7	0.2972265103917443	1.1672887585683507
8	0.1965040698868682	0.8819709020265949
9	0.1658319372416761	0.8373430192963494
>10	0.9097556341620047	9.433241760063634
>50	0.0934165946921372	3.6720046592560305
>100	0.08614642025535414	10.561420490539337
>500	0.00881538523541196	3.4145228348178263
>1k	0.0026780917170871775	2.0638264749932063
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1855	0.11557416372279647	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1822	0.11351812738702703	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1731	0.10784845143081438	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1617	0.10074578045270181	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1609	0.1002473474016062	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.230413138695227E-5
2	0.0	0.0	0.0	0.0	6.230413138695227E-5
3	0.0	0.0	0.0	0.0	6.230413138695227E-5
4	0.0	0.0	0.0	0.0	6.230413138695227E-5
5	0.0	0.0	0.0	0.0	1.2460826277390453E-4
6	0.0	0.0	0.0	0.0	1.869123941608568E-4
7	0.0	0.0	0.0	0.0	1.869123941608568E-4
8	0.0	0.0	0.0	0.0	1.869123941608568E-4
9	0.0	0.0	0.0	6.230413138695227E-5	1.869123941608568E-4
10	0.0	0.0	0.0	1.869123941608568E-4	1.869123941608568E-4
11	0.0	0.0	0.0	1.869123941608568E-4	1.869123941608568E-4
12	0.0	0.0	0.0	1.869123941608568E-4	4.3612891970866587E-4
13	0.0	0.0	0.0	1.869123941608568E-4	4.984330510956181E-4
14	0.0	0.0	0.0	1.869123941608568E-4	4.984330510956181E-4
15	0.0	0.0	0.0	1.869123941608568E-4	4.984330510956181E-4
16	0.0	0.0	0.0	1.869123941608568E-4	4.984330510956181E-4
17	0.0	0.0	0.0	1.869123941608568E-4	4.984330510956181E-4
18	0.0	0.0	0.0	3.1152065693476134E-4	4.984330510956181E-4
19	0.0	0.0	0.0	3.1152065693476134E-4	4.984330510956181E-4
20	0.0	0.0	0.0	3.1152065693476134E-4	5.607371824825705E-4
21	0.0	0.0	0.0	4.3612891970866587E-4	6.230413138695227E-4
22	0.0	0.0	0.0	8.099537080303795E-4	6.230413138695227E-4
23	0.0	0.0	0.0	9.968661021912363E-4	6.85345445256475E-4
24	0.0	0.0	0.0	0.00137069089051295	6.85345445256475E-4
25	0.0	0.0	0.0	0.0016822115474477112	6.85345445256475E-4
26	0.0	0.0	0.0	0.0019937322043824725	8.722578394173317E-4
27	0.0	0.0	0.0	0.0033644230948954223	8.722578394173317E-4
28	0.0	0.0	0.0	0.007538799897821224	9.345619708042841E-4
29	0.0	0.0	0.0	0.017133636131411875	9.345619708042841E-4
30	0.0	0.0	0.0	0.03482800944530632	9.345619708042841E-4
31	0.0	0.0	0.0	0.07725712291982081	9.345619708042841E-4
32	0.0	0.0	0.0	0.127910381737413	9.345619708042841E-4
33	0.0	0.0	0.0	0.18037046036522683	9.345619708042841E-4
34	0.0	0.0	0.0	0.2486557883653265	0.0010591702335781886
35	0.0	0.0	0.0	0.32011862706616073	0.0010591702335781886
36	0.0	0.0	0.0	0.42622256281814047	0.0010591702335781886
37	0.0	0.0	0.0	0.5778085144825953	0.0010591702335781886
38	0.0	0.0	0.0	0.784533622424503	0.0010591702335781886
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	125	9.968062E-10	21.119986	5
GGACCGT	115	8.776624E-9	21.043465	6
GTATCAA	2420	0.0	21.000639	1
AACGAAT	55	0.00448426	19.999987	31
ATGCGTA	75	0.001291127	17.599987	22
ATTTCGT	75	0.001291127	17.599987	42
ACCGTCG	465	0.0	17.505363	8
ATACCGT	460	0.0	17.21738	6
ATAGCGT	410	0.0	17.17072	6
TAGTACG	200	7.2759576E-11	16.499989	2
TACCGTC	480	0.0	16.041656	7
GTATTAG	550	0.0	16.000486	1
TAGCGTA	400	0.0	15.949988	7
TAGACAG	320	0.0	15.812488	5
AACGCAG	3160	0.0	15.803786	6
AATACTG	265	0.0	15.773575	5
CGTATAT	405	0.0	15.753076	10
CGCTTCG	420	0.0	15.714275	32
GTTTAGA	255	0.0	15.529885	1
TCAACGC	3225	0.0	15.48526	4
>>END_MODULE