FastQCFastQC Report
Thu 2 Feb 2017
SRR4062366_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062366_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468315
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG54260.3695392337475269No Hit
TCCATGTACTCTGCGTTGATACCAC47800.32554322471676717No Hit
GTATCAACGCAGAGTACTTTTTTTT36560.2489928932143307No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30970.21092204329452466No Hit
TATCAACGCAGAGTACTTTTTTTTT26510.18054708969124472No Hit
GAGTACATGGAAGCAGTGGTATCAA21190.14431508225414846No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20410.13900287063743133No Hit
CATGTACTCTGCGTTGATACCACTG17560.11959286665327264No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15700.10692528510571642No Hit
GCTTCCATGTACTCTGCGTTGATAC14830.1010001259947627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAT1650.012.6587591
CGCGTAA701.0905815E-412.2145710
TACCGTC1701.8189894E-1211.7359697
CGAGATT658.025969E-411.6917834
TACCGGC658.0535666E-411.6872
CGCATCG1900.011.50105413
GGACAAT1001.9278104E-611.4006566
GCGTAAC752.0745271E-411.39948811
CGTCGTA1701.0913936E-1111.17673110
GATCGAT600.005872877311.083977
CATTCCG600.005872877311.083979
GACGGTC600.005872877311.083977
AGGGACG2950.010.9497828
ACACCGT700.001490600310.8577666
AATACTA1053.4656186E-610.8570265
TTATACT1408.35098E-910.8566554
CTATTAG700.001499104410.8503661
GATTAAG3600.010.812691
GACGGCC2900.010.8098611
GCGTTAT803.79222E-410.6808281
Produced by FastQC (version 0.11.5)