##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062366_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1468315 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.09984437944174 32.0 32.0 32.0 32.0 32.0 2 30.791090467644885 32.0 32.0 32.0 32.0 32.0 3 30.7886230134542 32.0 32.0 32.0 32.0 32.0 4 30.835739606283393 32.0 32.0 32.0 32.0 32.0 5 30.671017458787794 32.0 32.0 32.0 32.0 32.0 6 34.279280672062875 36.0 36.0 36.0 32.0 36.0 7 34.15112424786235 36.0 36.0 36.0 32.0 36.0 8 34.14284400826798 36.0 36.0 36.0 32.0 36.0 9 34.28842584867689 36.0 36.0 36.0 32.0 36.0 10 33.98921893462915 36.0 36.0 36.0 32.0 36.0 11 34.30194678934697 36.0 36.0 36.0 32.0 36.0 12 34.09120999240626 36.0 36.0 36.0 32.0 36.0 13 34.1955915454109 36.0 36.0 36.0 32.0 36.0 14 34.103623541270096 36.0 36.0 36.0 32.0 36.0 15 34.05560795878269 36.0 36.0 36.0 32.0 36.0 16 34.046198533693385 36.0 36.0 36.0 32.0 36.0 17 33.98881438928295 36.0 36.0 36.0 32.0 36.0 18 33.9998072620657 36.0 36.0 36.0 32.0 36.0 19 33.98530560540483 36.0 36.0 36.0 32.0 36.0 20 33.964834521202874 36.0 36.0 36.0 32.0 36.0 21 33.92911670860817 36.0 36.0 36.0 32.0 36.0 22 33.890065823750355 36.0 36.0 36.0 32.0 36.0 23 33.84694088121418 36.0 36.0 36.0 32.0 36.0 24 33.82837878793038 36.0 36.0 36.0 32.0 36.0 25 33.34019335088179 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 53.0 5 150.0 6 257.0 7 52.0 8 249.0 9 271.0 10 159.0 11 54.0 12 114.0 13 82.0 14 279.0 15 358.0 16 504.0 17 728.0 18 1035.0 19 1381.0 20 2180.0 21 3229.0 22 4813.0 23 6956.0 24 9630.0 25 13124.0 26 18138.0 27 23271.0 28 31228.0 29 41871.0 30 53654.0 31 73095.0 32 104911.0 33 149925.0 34 328472.0 35 598084.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.064071914202074 17.632479086551232 11.473211305862849 25.830237693383847 2 16.026556550154844 20.629274183167457 37.61356105820279 25.730608208474916 3 18.842372248754295 25.084522736003006 28.409378822593517 27.66372619264918 4 12.437623543868206 16.17237766858161 36.39222615322368 34.997772634326495 5 14.10949067229513 37.00838955209848 34.019994849357325 14.862124926249065 6 33.004521643381615 35.66040795634802 17.790445405040035 13.544624995230334 7 29.082673725504094 30.982984912280227 21.835552169106773 18.09878919310891 8 27.617697903714856 33.00088072333835 20.468024016752832 18.91339735619397 9 27.275131878365134 14.972574481110273 19.01598083554096 38.73631280498363 10 15.551709818698054 27.41819456291905 32.50970468968518 24.52039092869772 11 36.25092905117452 21.737751866625754 22.694759810439464 19.31655927176025 12 24.06920679519617 24.3242653427264 29.59538439505432 22.01114346702311 13 29.400601573692775 20.407272618168452 25.501010237390442 24.691115570748327 14 23.382306310115208 20.020525689502914 25.939561732204535 30.65760626817734 15 24.847045539620883 27.289271266516796 23.56565774338561 24.29802545047671 16 25.109230152431287 26.136075405624332 24.700946661321073 24.053747780623308 17 23.31611729454974 25.95028033457396 26.402116113475838 24.331486257400464 18 24.17455467961483 25.26685125071606 27.284500395537247 23.274093674131862 19 24.651631923630106 26.121485033795093 26.090932711920928 23.135950330653866 20 24.654271351301222 24.829726935739476 26.59696631634649 23.919035396612813 21 25.660991381367985 25.18348179639624 24.913970531070856 24.241556291164915 22 24.680807234717694 25.394361143814432 25.57191403793343 24.352917583534442 23 23.667906162101996 25.18912990694236 25.87287578400587 25.270088146949774 24 23.78103589613486 26.13119132283879 25.781600588152653 24.306172192873696 25 24.278851727632436 25.252976454333297 25.845062066011888 24.62310975202238 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 369.0 1 369.0 2 768.5 3 1168.0 4 1168.0 5 1168.0 6 3107.0 7 5046.0 8 5046.0 9 5046.0 10 5028.0 11 5010.0 12 5010.0 13 5010.0 14 5330.0 15 5650.0 16 5650.0 17 5650.0 18 9040.5 19 12431.0 20 12431.0 21 12431.0 22 19230.0 23 26029.0 24 26029.0 25 26029.0 26 37959.0 27 49889.0 28 49889.0 29 49889.0 30 61402.5 31 72916.0 32 72916.0 33 72916.0 34 86304.0 35 99692.0 36 99692.0 37 99692.0 38 112300.5 39 124909.0 40 124909.0 41 124909.0 42 141664.0 43 158419.0 44 158419.0 45 158419.0 46 183761.5 47 209104.0 48 209104.0 49 209104.0 50 201534.5 51 193965.0 52 193965.0 53 193965.0 54 175987.0 55 158009.0 56 158009.0 57 158009.0 58 142514.0 59 127019.0 60 127019.0 61 127019.0 62 110582.0 63 94145.0 64 94145.0 65 94145.0 66 77231.5 67 60318.0 68 60318.0 69 60318.0 70 45496.5 71 30675.0 72 30675.0 73 30675.0 74 23498.0 75 16321.0 76 16321.0 77 16321.0 78 13219.5 79 10118.0 80 10118.0 81 10118.0 82 7066.0 83 4014.0 84 4014.0 85 4014.0 86 2874.0 87 1734.0 88 1734.0 89 1734.0 90 1174.0 91 614.0 92 614.0 93 614.0 94 372.0 95 130.0 96 130.0 97 130.0 98 375.5 99 621.0 100 621.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008240738533625277 2 0.0035414744111447478 3 0.004835474676755328 4 0.014506424030265986 5 0.036572533822783256 6 0.04815043093614109 7 0.07008033017438356 8 0.09119296608697725 9 0.09759486213789276 10 0.11959286665327264 11 0.12020581414750922 12 0.13518897511773698 13 0.12449644660716536 14 0.12667581547556211 15 0.1159151816878531 16 0.1148936025307921 17 0.12619907853560033 18 0.13314581680361504 19 0.1347122381777752 20 0.12579044687277594 21 0.13328202735788983 22 0.1548032949333079 23 0.14485992447124765 24 0.13818560731178256 25 0.1343717117920882 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1468315.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.232926614321656 #Duplication Level Percentage of deduplicated Percentage of total 1 80.41754275068186 46.0253132275385 2 12.3585540118328 14.146324296367144 3 3.3938757735928733 5.827243292644952 4 1.3268030100039363 3.0374727721286554 5 0.6486182766397435 1.8561161113815112 6 0.3776229581941609 1.296748023252568 7 0.2580738884121102 1.0339226741594056 8 0.17171895058435205 0.786238247766604 9 0.13498236616903098 0.695289227146168 >10 0.7449574380468897 8.103882334891845 >50 0.08199930411022914 3.3420198743549454 >100 0.0771321609632869 8.953542810862189 >500 0.005969934388870958 2.321420628502585 >1k 0.002029777692216126 2.2036787974434473 >5k 1.1939868777741916E-4 0.37078768155941905 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGAAGCAGTGGTATCAACG 5426 0.3695392337475269 No Hit TCCATGTACTCTGCGTTGATACCAC 4780 0.32554322471676717 No Hit GTATCAACGCAGAGTACTTTTTTTT 3656 0.2489928932143307 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3097 0.21092204329452466 No Hit TATCAACGCAGAGTACTTTTTTTTT 2651 0.18054708969124472 No Hit GAGTACATGGAAGCAGTGGTATCAA 2119 0.14431508225414846 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2041 0.13900287063743133 No Hit CATGTACTCTGCGTTGATACCACTG 1756 0.11959286665327264 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1570 0.10692528510571642 No Hit GCTTCCATGTACTCTGCGTTGATAC 1483 0.1010001259947627 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.8105277137399E-5 0.0 10 0.0 0.0 0.0 2.0431583141219698E-4 0.0 11 0.0 0.0 0.0 2.0431583141219698E-4 0.0 12 0.0 0.0 0.0 2.0431583141219698E-4 0.0 13 0.0 0.0 0.0 2.0431583141219698E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAAT 165 0.0 12.658759 1 CGCGTAA 70 1.0905815E-4 12.21457 10 TACCGTC 170 1.8189894E-12 11.735969 7 CGAGATT 65 8.025969E-4 11.691783 4 TACCGGC 65 8.0535666E-4 11.687 2 CGCATCG 190 0.0 11.501054 13 GGACAAT 100 1.9278104E-6 11.400656 6 GCGTAAC 75 2.0745271E-4 11.399488 11 CGTCGTA 170 1.0913936E-11 11.176731 10 GATCGAT 60 0.0058728773 11.08397 7 CATTCCG 60 0.0058728773 11.08397 9 GACGGTC 60 0.0058728773 11.08397 7 AGGGACG 295 0.0 10.949782 8 ACACCGT 70 0.0014906003 10.857766 6 AATACTA 105 3.4656186E-6 10.857026 5 TTATACT 140 8.35098E-9 10.856655 4 CTATTAG 70 0.0014991044 10.850366 1 GATTAAG 360 0.0 10.81269 1 GACGGCC 290 0.0 10.80986 11 GCGTTAT 80 3.79222E-4 10.680828 1 >>END_MODULE