FastQCFastQC Report
Thu 2 Feb 2017
SRR4062366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468315
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA26280.17898066831708456No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC23000.15664213741601768No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGCG654.9330643E-420.3075669
ATGCGTT1001.1238408E-619.79987540
TTAACGG1851.8189894E-1219.02690935
GTCCTAA2850.018.5299871
TAACGCT600.007413107418.333224
TAGGACG3450.017.8549614
TAACGGC2003.6379788E-1217.5998936
ACGATTA1307.3199044E-716.92297240
ATAAGGG1702.4683686E-916.8234253
GGCGTTA2500.016.71989442
CGTGATT2251.8189894E-1216.62211828
CGATTAA1351.0859039E-616.29619441
CCGTGAT2301.8189894E-1216.26076927
TATACTG2600.016.0768245
AGGACGT3700.015.4593645
CGAGCCG3450.015.30425315
AATCCCG1301.3694222E-515.23067519
GGACGTG3900.015.2306756
ATACCGT3050.015.1474486
TACCGTC3200.015.1249077
Produced by FastQC (version 0.11.5)