##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062366_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1468315
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22612858957376	32.0	32.0	32.0	32.0	32.0
2	31.35121618998648	32.0	32.0	32.0	32.0	32.0
3	31.45822183931922	32.0	32.0	32.0	32.0	32.0
4	31.575116374892307	32.0	32.0	32.0	32.0	32.0
5	31.501002850205847	32.0	32.0	32.0	32.0	32.0
6	35.0712932851602	36.0	36.0	36.0	36.0	36.0
7	35.087656940098	36.0	36.0	36.0	36.0	36.0
8	35.029377211293216	36.0	36.0	36.0	36.0	36.0
9	35.16790402604346	36.0	36.0	36.0	36.0	36.0
10	35.032921409915446	36.0	36.0	36.0	36.0	36.0
11	35.17866125456731	36.0	36.0	36.0	36.0	36.0
12	35.08224733793498	36.0	36.0	36.0	36.0	36.0
13	35.125814964772545	36.0	36.0	36.0	36.0	36.0
14	35.08921586989168	36.0	36.0	36.0	36.0	36.0
15	35.0448990850056	36.0	36.0	36.0	36.0	36.0
16	35.060617101916144	36.0	36.0	36.0	36.0	36.0
17	35.03829832154545	36.0	36.0	36.0	36.0	36.0
18	35.045861412571554	36.0	36.0	36.0	36.0	36.0
19	35.027504316171935	36.0	36.0	36.0	36.0	36.0
20	35.00793290268096	36.0	36.0	36.0	36.0	36.0
21	34.99568621174612	36.0	36.0	36.0	36.0	36.0
22	34.97748575748392	36.0	36.0	36.0	36.0	36.0
23	34.9300531561688	36.0	36.0	36.0	32.0	36.0
24	34.92097404167362	36.0	36.0	36.0	32.0	36.0
25	34.89142179981816	36.0	36.0	36.0	32.0	36.0
26	34.84470907128239	36.0	36.0	36.0	32.0	36.0
27	34.82633358645795	36.0	36.0	36.0	32.0	36.0
28	34.8047414893943	36.0	36.0	36.0	32.0	36.0
29	34.78135686143641	36.0	36.0	36.0	32.0	36.0
30	34.758175868257155	36.0	36.0	36.0	32.0	36.0
31	34.75072174567446	36.0	36.0	36.0	32.0	36.0
32	34.71383524652408	36.0	36.0	36.0	32.0	36.0
33	34.68011019433841	36.0	36.0	36.0	32.0	36.0
34	34.65737256651332	36.0	36.0	36.0	32.0	36.0
35	34.61949990294998	36.0	36.0	36.0	32.0	36.0
36	34.58801960069876	36.0	36.0	36.0	32.0	36.0
37	34.570097015967285	36.0	36.0	36.0	32.0	36.0
38	34.52264262096349	36.0	36.0	36.0	32.0	36.0
39	34.48885967929225	36.0	36.0	36.0	32.0	36.0
40	34.455429522956585	36.0	36.0	36.0	32.0	36.0
41	34.43214432870331	36.0	36.0	36.0	32.0	36.0
42	34.37611275509683	36.0	36.0	36.0	32.0	36.0
43	34.37402124203594	36.0	36.0	36.0	32.0	36.0
44	34.347903549306515	36.0	36.0	36.0	32.0	36.0
45	34.27345903297317	36.0	36.0	36.0	32.0	36.0
46	34.2892866993799	36.0	36.0	36.0	32.0	36.0
47	34.238129420458144	36.0	36.0	36.0	32.0	36.0
48	34.20703936144492	36.0	36.0	36.0	32.0	36.0
49	34.199101010341785	36.0	36.0	36.0	32.0	36.0
50	33.66374177203121	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	9.0
21	30.0
22	138.0
23	427.0
24	1143.0
25	2556.0
26	5251.0
27	9965.0
28	16936.0
29	25544.0
30	36994.0
31	52474.0
32	77333.0
33	121729.0
34	259863.0
35	857920.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.57193602417246	18.2539947429258	12.023632993828148	26.15043623907359
2	15.54955750163119	21.17957792044156	37.257381897137684	26.01348268078956
3	18.184827202937797	25.49321942475441	28.836446204893782	27.48550716741401
4	12.267479753976161	16.6857475211348	36.460550305009896	34.58622241987914
5	13.905463064805575	37.26073764825667	33.889254008846876	14.944545278090873
6	33.11328368424816	35.97263511327687	17.543630240311106	13.370450962163854
7	29.06721586799554	31.095051875824925	21.770411505985777	18.067320750193762
8	27.728995481214863	33.24579535045273	20.185518774922276	18.839690393410134
9	27.419388891348245	14.704950913121504	18.82327702162002	39.05238317391023
10	15.472224965351442	27.47945774578343	32.451347292644975	24.596969996220157
11	36.413712316498845	21.612801067890747	22.675652022896994	19.297834592713418
12	24.11662347657008	24.29846456652694	29.5572135406912	22.02769841621178
13	29.56661206893616	20.364090811576535	25.467083016927567	24.602214102559735
14	23.48957818996605	19.92195135240054	25.859028886853295	30.729441570780114
15	24.969301546330318	27.297752866380854	23.39967922414468	24.333266363144148
16	25.154003057926943	26.049723662838016	24.659150114246604	24.137123164988438
17	23.508920088673072	26.007566496289964	26.30695729458597	24.176556120450993
18	24.360236052890556	25.126624736517712	27.231690747557575	23.281448463034156
19	24.820355305230827	26.061778296891337	26.02234534142878	23.09552105644906
20	24.8623762612246	24.717380126199078	26.58686998362068	23.83337362895564
21	25.880754470260126	25.030460085199703	24.911752587149216	24.177032857390955
22	24.819538041905176	25.36587857510139	25.445766065183562	24.36881731780987
23	23.855303528193883	25.0666920926368	25.81244487729132	25.265559501878005
24	23.962909866070973	25.939188798044015	25.756189918375828	24.341711417509185
25	24.53376829903665	24.959766807531082	25.80325066487777	24.703214228554497
26	23.662838015003594	25.555143140266225	26.375947940326157	24.40607090440403
27	24.772000558463272	25.678822323547738	25.609218730313316	23.93995838767567
28	23.977211974269828	25.11334420747592	26.201870851962966	24.707572966291295
29	23.882000796831743	25.773216237660172	26.221417066501395	24.123365899006686
30	24.543643564221572	25.263175817178197	26.31928434974784	23.873896268852395
31	23.981434501452345	25.4614983842023	25.70211432832873	24.854952786016625
32	23.888470798159794	25.759527076955557	25.73569022995747	24.61631189492718
33	23.735710661540608	25.22551359892121	26.09378777714591	24.944987962392265
34	23.940367019338492	25.687880325407015	26.655111471312352	23.716641183942137
35	24.987485655326005	25.347285834442886	25.90030068479856	23.76492782543255
36	24.12350210956096	25.778800870385442	25.84779151612563	24.24990550392797
37	24.790797614953195	25.609831677807556	25.702795381100103	23.896575326139146
38	23.929265859165096	25.69319253702373	25.7987557165867	24.578785887224473
39	24.084205364652682	25.64660852746175	25.754895918110215	24.514290189775352
40	24.82369246381056	25.424653429270965	25.83308077626395	23.918573330654525
41	23.59827421227734	25.626176944320534	26.397673523732983	24.377875319669144
42	24.911275850209254	25.92522721623085	25.74883454844499	23.41466238511491
43	24.15592022147836	24.94355775157238	26.007430285735687	24.893091741213567
44	23.879617112131935	25.54976282337237	26.199963904203116	24.37065616029258
45	24.048381988878408	25.72649601754392	25.954716801231342	24.270405192346328
46	23.84054091972153	25.355407630792868	26.237712097003772	24.566339352481826
47	24.239417291248813	25.170416429717058	26.361509621573028	24.228656657461105
48	24.204819810463015	26.53633586798473	25.345038360297345	23.91380596125491
49	23.552507466041007	25.682091376850337	26.363416569332877	24.401984587775786
50	23.475207976490058	26.577062823712893	25.346059939454406	24.601669260342636
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	65.0
2	75.0
3	240.0
4	405.0
5	416.0
6	427.0
7	368.0
8	309.0
9	449.0
10	589.0
11	866.0
12	1143.0
13	1879.0
14	2615.0
15	3421.0
16	4227.0
17	4942.5
18	5658.0
19	6056.5
20	6455.0
21	7398.5
22	8342.0
23	9126.5
24	9911.0
25	11370.0
26	12829.0
27	15576.0
28	18323.0
29	21202.5
30	24082.0
31	26191.0
32	28300.0
33	32674.0
34	37048.0
35	42428.0
36	47808.0
37	54684.5
38	61561.0
39	65203.5
40	68846.0
41	75109.0
42	81372.0
43	83472.0
44	85572.0
45	94534.0
46	103496.0
47	109717.0
48	115938.0
49	114682.0
50	113426.0
51	106929.5
52	100433.0
53	97345.0
54	94257.0
55	93656.0
56	93055.0
57	89377.5
58	85700.0
59	78269.5
60	70839.0
61	62542.5
62	54246.0
63	46924.5
64	39603.0
65	33733.5
66	27864.0
67	24186.5
68	20509.0
69	18301.5
70	16094.0
71	12711.5
72	9329.0
73	8110.5
74	6892.0
75	5094.5
76	3297.0
77	2994.5
78	2692.0
79	2311.0
80	1930.0
81	1543.5
82	1157.0
83	959.5
84	762.0
85	627.5
86	493.0
87	359.0
88	225.0
89	150.5
90	76.0
91	49.0
92	22.0
93	17.0
94	12.0
95	8.5
96	5.0
97	5.5
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013484844873204999
2	0.004154421905381339
3	2.0431583141219698E-4
4	1.36210554274798E-4
5	0.0
6	6.810527713739899E-4
7	6.8105277137399E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	6.8105277137399E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1468315.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.90687254739115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.08420631784729	49.38585419807494
2	12.203517599872297	14.865561821705336
3	3.1626753855565974	5.778859998506005
4	1.2445528889061808	3.0320729673238485
5	0.6207600906338027	1.890427786137
6	0.3465474112896765	1.26642714066292
7	0.23376303629194026	0.9966442820407065
8	0.15032611785301994	0.7324714960494391
9	0.12601453921303846	0.6907636331070081
>10	0.6847415287393721	7.810901085075786
>50	0.07112526582143375	3.0290779230870566
>100	0.06615411030020305	7.887266692669938
>500	0.005054136907596734	2.0806000706924537
>1k	5.615707675107482E-4	0.5530709048676143
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2628	0.17898066831708456	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2300	0.15664213741601768	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.36210554274798E-4	0.0
10	0.0	0.0	0.0	4.7673693996179293E-4	6.8105277137399E-5
11	0.0	0.0	0.0	4.7673693996179293E-4	6.8105277137399E-5
12	0.0	0.0	0.0	4.7673693996179293E-4	1.36210554274798E-4
13	0.0	0.0	0.0	4.7673693996179293E-4	1.36210554274798E-4
14	0.0	0.0	0.0	4.7673693996179293E-4	1.36210554274798E-4
15	0.0	0.0	0.0	5.44842217099192E-4	1.36210554274798E-4
16	0.0	0.0	0.0	6.810527713739899E-4	2.0431583141219698E-4
17	0.0	0.0	0.0	7.491580485113889E-4	2.0431583141219698E-4
18	0.0	0.0	0.0	7.491580485113889E-4	2.0431583141219698E-4
19	0.0	0.0	0.0	9.534738799235859E-4	2.0431583141219698E-4
20	0.0	0.0	0.0	0.0011577897113357828	2.0431583141219698E-4
21	0.0	0.0	0.0	0.0014302108198853789	2.0431583141219698E-4
22	6.8105277137399E-5	0.0	0.0	0.0023836846998089647	2.0431583141219698E-4
23	6.8105277137399E-5	0.0	0.0	0.0037457902425569447	2.72421108549596E-4
24	1.36210554274798E-4	0.0	0.0	0.006470001328052904	2.72421108549596E-4
25	1.36210554274798E-4	0.0	0.0	0.008376949087900076	2.72421108549596E-4
26	1.36210554274798E-4	0.0	0.0	0.01076063378770904	2.72421108549596E-4
27	1.36210554274798E-4	0.0	0.0	0.014370213475991187	2.72421108549596E-4
28	1.36210554274798E-4	0.0	0.0	0.019886740924120506	2.72421108549596E-4
29	1.36210554274798E-4	0.0	0.0	0.029898216663318156	2.72421108549596E-4
30	1.36210554274798E-4	0.0	0.0	0.04787800982759149	2.72421108549596E-4
31	1.36210554274798E-4	0.0	0.0	0.08846875500148128	2.72421108549596E-4
32	1.36210554274798E-4	0.0	0.0	0.13668729121475978	2.72421108549596E-4
33	1.36210554274798E-4	0.0	0.0	0.18490582742803827	2.72421108549596E-4
34	1.36210554274798E-4	0.0	0.0	0.24177373383776643	2.72421108549596E-4
35	1.36210554274798E-4	0.0	0.0	0.30769964210676864	2.72421108549596E-4
36	1.36210554274798E-4	0.0	0.0	0.4033194512076768	2.72421108549596E-4
37	1.36210554274798E-4	0.0	0.0	0.5471577965218635	2.72421108549596E-4
38	1.36210554274798E-4	0.0	0.0	0.729884255081505	2.72421108549596E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGCGCG	65	4.9330643E-4	20.307566	9
ATGCGTT	100	1.1238408E-6	19.799875	40
TTAACGG	185	1.8189894E-12	19.026909	35
GTCCTAA	285	0.0	18.529987	1
TAACGCT	60	0.0074131074	18.33322	4
TAGGACG	345	0.0	17.854961	4
TAACGGC	200	3.6379788E-12	17.59989	36
ACGATTA	130	7.3199044E-7	16.922972	40
ATAAGGG	170	2.4683686E-9	16.823425	3
GGCGTTA	250	0.0	16.719894	42
CGTGATT	225	1.8189894E-12	16.622118	28
CGATTAA	135	1.0859039E-6	16.296194	41
CCGTGAT	230	1.8189894E-12	16.260769	27
TATACTG	260	0.0	16.076824	5
AGGACGT	370	0.0	15.459364	5
CGAGCCG	345	0.0	15.304253	15
AATCCCG	130	1.3694222E-5	15.230675	19
GGACGTG	390	0.0	15.230675	6
ATACCGT	305	0.0	15.147448	6
TACCGTC	320	0.0	15.124907	7
>>END_MODULE