FastQCFastQC Report
Thu 2 Feb 2017
SRR4062365_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062365_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883315
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG305413.4575434584491376No Hit
TCCATGTACTCTGCGTTGATACCAC257192.911645336035276No Hit
GAGTACATGGAAGCAGTGGTATCAA111091.2576487436531703No Hit
CATGTACTCTGCGTTGATACCACTG90281.0220589483932685No Hit
GCTTCCATGTACTCTGCGTTGATAC72380.8194132331048379No Hit
CCCATGTACTCTGCGTTGATACCAC71200.8060544652813549No Hit
GCGTTGATACCACTGCTTCCATGTA67620.7655253222236689No Hit
GTACATGGGAAGCAGTGGTATCAAC63590.7199017338095697No Hit
CATGGAAGCAGTGGTATCAACGCAG63510.7189960546350962No Hit
GTATCAACGCAGAGTACATGGAAGC49590.5614078782767189No Hit
CAGTGGTATCAACGCAGAGTACATG40240.45555662476013653No Hit
ACTCTGCGTTGATACCACTGCTTCC39110.4427639064206993No Hit
ACGCAGAGTACATGGAAGCAGTGGT37440.4238578536535664No Hit
GTATCAACGCAGAGTACTTTTTTTT36780.4163860004641606No Hit
GTACTTTTTTTTTTTTTTTTTTTTT35460.4014422940853489No Hit
TATCAACGCAGAGTACATGGAAGCA30540.34574302485523284No Hit
TATCAACGCAGAGTACTTTTTTTTT28750.3254784533263898No Hit
GTATCAACGCAGAGTACATGGGAAG23460.2655904179143341No Hit
GGTATCAACGCAGAGTACATGGAAG21910.2480428839089113No Hit
ATACCACTGCTTCCATGTACTCTGC21360.24181633958440646No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21280.24091066040993303No Hit
GTACTCTGCGTTGATACCACTGCTT20930.23694831402161176No Hit
AAGCAGTGGTATCAACGCAGAGTAC19400.21962719980980738No Hit
GAGTACATGGGAAGCAGTGGTATCA18720.2119289268267832No Hit
GTGGTATCAACGCAGAGTACATGGA18110.20502312312142326No Hit
GCGTTGATACCACTGCTTCCCATGT18030.20411744394694983No Hit
CCACTGCTTCCATGTACTCTGCGTT17670.2000418876618194No Hit
GAAGCAGTGGTATCAACGCAGAGTA17490.19800410951925418No Hit
AAAAAGTACTCTGCGTTGATACCAC17270.19551349178945224No Hit
ACATGGAAGCAGTGGTATCAACGCA16310.18464534169577104No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15910.18011694582340387No Hit
TGGAAGCAGTGGTATCAACGCAGAG15210.17219225304676136No Hit
GCTTCCCATGTACTCTGCGTTGATA15080.170720524388242No Hit
GTACATGGGGTGGTATCAACGCAAA14880.16845632645205844No Hit
CTGCTTCCATGTACTCTGCGTTGAT14870.16834311655524925No Hit
TATCAACGCAGAGTACATGGGAAGC14090.1595127446041333No Hit
ACGCAGAGTACATGGGAAGCAGTGG12890.1459275569870318No Hit
GCAGAGTACATGGAAGCAGTGGTAT12740.14422940853489413No Hit
GGTATCAACGCAGAGTACTTTTTTT12600.14264446997956562No Hit
GTTGATACCACTGCTTCCATGTACT12490.14139916111466463No Hit
GATACCACTGCTTCCATGTACTCTG11580.13109706050502937No Hit
GGTATCAACGCAGAGTACATGGGAA11490.13007817143374675No Hit
CCCCATGTACTCTGCGTTGATACCA10890.123285577625196No Hit
CTTCCATGTACTCTGCGTTGATACC10670.12079495989539406No Hit
GCAGTGGTATCAACGCAGAGTACAT10660.12068174999858487No Hit
CCATGTACTCTGCGTTGATACCACT9980.11298347701556069No Hit
GTACATGGGTGGTATCAACGCAAAA9280.10505878423891817No Hit
ATCAACGCAGAGTACATGGAAGCAG8950.10132285764421527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGGT704.444355E-714.9242064
CATCGCC653.364481E-614.61691115
TTAGGAC1450.014.4079463
GCATCGC602.5632486E-514.2514914
CTAGGGC400.00529093214.2442193
CGTCAAT759.635351E-713.9363719
ATACCAA1900.013.50294219
ATCAACC1850.013.3542619
AACCGCG500.001497471213.301397
ATCGCCA801.9929957E-613.06460616
CGACCAT1102.732122E-912.95663410
GTGCTAG604.1127478E-412.6579421
GCGTAGG751.4873689E-512.6579411
TACGCTA1301.9645086E-1012.4250799
ATACGCT1301.9645086E-1012.4243758
AAAAAGT3450.012.3827691
TCCCGGA701.08823035E-412.21625417
GGTTCCG701.0888067E-412.2155628
CGGTCCA1103.7965947E-812.09285710
AAGACTG550.003069447412.0894315
Produced by FastQC (version 0.11.5)