##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062365_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	883315
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.002424955989653	32.0	32.0	32.0	32.0	32.0
2	30.797307868653878	32.0	32.0	32.0	32.0	32.0
3	30.78688350135569	32.0	32.0	32.0	32.0	32.0
4	30.80498349965754	32.0	32.0	32.0	32.0	32.0
5	30.706969767297057	32.0	32.0	32.0	32.0	32.0
6	34.29474762683754	36.0	36.0	36.0	32.0	36.0
7	34.12627431890096	36.0	36.0	36.0	32.0	36.0
8	34.1232074627964	36.0	36.0	36.0	32.0	36.0
9	34.221249497631085	36.0	36.0	36.0	32.0	36.0
10	34.01006775612324	36.0	36.0	36.0	32.0	36.0
11	34.27600346422284	36.0	36.0	36.0	32.0	36.0
12	34.083608905090486	36.0	36.0	36.0	32.0	36.0
13	34.13728511346462	36.0	36.0	36.0	32.0	36.0
14	34.03716907332039	36.0	36.0	36.0	32.0	36.0
15	34.01926832443692	36.0	36.0	36.0	32.0	36.0
16	33.965923821060436	36.0	36.0	36.0	32.0	36.0
17	33.882503976497624	36.0	36.0	36.0	32.0	36.0
18	33.87771746206053	36.0	36.0	36.0	32.0	36.0
19	33.88352852606375	36.0	36.0	36.0	32.0	36.0
20	33.87059655955124	36.0	36.0	36.0	32.0	36.0
21	33.70671844132614	36.0	36.0	36.0	27.0	36.0
22	33.64725607512609	36.0	36.0	36.0	27.0	36.0
23	33.467255735496394	36.0	36.0	36.0	27.0	36.0
24	33.448544403751775	36.0	36.0	36.0	27.0	36.0
25	32.78172113006119	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	4.0
4	49.0
5	76.0
6	137.0
7	37.0
8	126.0
9	164.0
10	104.0
11	41.0
12	72.0
13	52.0
14	275.0
15	339.0
16	449.0
17	625.0
18	954.0
19	1218.0
20	1748.0
21	2502.0
22	3593.0
23	4991.0
24	6331.0
25	8548.0
26	11337.0
27	14076.0
28	18721.0
29	25432.0
30	33053.0
31	46059.0
32	66068.0
33	92721.0
34	195449.0
35	347963.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.12588239956071	15.792900044721453	13.031378609559068	24.049838946158765
2	13.446629200762603	21.494703903126034	36.88134841026523	28.177318485846133
3	19.70437159150476	27.559265502886433	26.58663083017556	26.149732075433246
4	12.731775032156378	17.420605445750827	32.95802460189496	36.88959492019783
5	13.300763178964845	37.83222746560938	33.57591076955483	15.291098585870941
6	34.05736614095195	31.605289237477702	19.16810601126936	15.169238610300988
7	28.571849341139366	30.884432038570985	22.606872269851916	17.93684635043773
8	30.087360489490678	31.18259588691859	21.247294770834515	17.48274885275622
9	27.20252111696088	17.111820738702683	17.3740404729474	38.31161767138904
10	15.90278745921047	28.254973233908327	31.903617134379044	23.93862217250216
11	35.8964797305109	22.11735289517173	19.247683794609767	22.738483579707616
12	22.8563946825189	24.303772500501616	29.424914441697357	23.414918375282124
13	32.408001432713476	20.758816741891682	23.58411864318407	23.249063182210776
14	26.1892320474616	18.65706069512564	23.572111095871932	31.581596161540826
15	27.520981973353887	24.113770181513196	26.993613319657033	21.371634525475887
16	26.877318490460826	24.516694264707716	26.212018691864188	22.393968552967273
17	24.755166441480245	24.16903943633874	28.59950670571913	22.476287416461883
18	27.34772717225338	21.533060600805047	30.019735134320204	21.09947709262137
19	23.272184808126333	31.285615341278145	24.483970758437444	20.958229092158078
20	23.248836487040972	21.99645902144763	32.44925995361817	22.305444537893234
21	24.25710868912202	27.390542907180386	22.20892206371607	26.143426339981524
22	22.57395949900397	27.68369268585254	24.17225338235811	25.57009443278538
23	21.686178793584862	26.718709082066567	23.714064489213822	27.881047635134752
24	21.274707186679546	29.9179714600536	24.24433078042742	24.56299057283944
25	26.396129548579584	24.033095624317735	22.590744878260562	26.980029948842116
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	306.0
1	306.0
2	672.0
3	1038.0
4	1038.0
5	1038.0
6	2798.5
7	4559.0
8	4559.0
9	4559.0
10	4088.0
11	3617.0
12	3617.0
13	3617.0
14	2921.5
15	2226.0
16	2226.0
17	2226.0
18	3654.5
19	5083.0
20	5083.0
21	5083.0
22	7961.0
23	10839.0
24	10839.0
25	10839.0
26	16296.0
27	21753.0
28	21753.0
29	21753.0
30	26871.5
31	31990.0
32	31990.0
33	31990.0
34	37264.0
35	42538.0
36	42538.0
37	42538.0
38	49541.5
39	56545.0
40	56545.0
41	56545.0
42	76275.0
43	96005.0
44	96005.0
45	96005.0
46	159421.0
47	222837.0
48	222837.0
49	222837.0
50	174722.0
51	126607.0
52	126607.0
53	126607.0
54	101901.0
55	77195.0
56	77195.0
57	77195.0
58	69899.0
59	62603.0
60	62603.0
61	62603.0
62	55518.5
63	48434.0
64	48434.0
65	48434.0
66	40595.0
67	32756.0
68	32756.0
69	32756.0
70	24772.0
71	16788.0
72	16788.0
73	16788.0
74	13133.5
75	9479.0
76	9479.0
77	9479.0
78	7696.5
79	5914.0
80	5914.0
81	5914.0
82	4135.0
83	2356.0
84	2356.0
85	2356.0
86	1657.5
87	959.0
88	959.0
89	959.0
90	667.0
91	375.0
92	375.0
93	375.0
94	233.0
95	91.0
96	91.0
97	91.0
98	256.5
99	422.0
100	422.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007924692776642534
2	0.0026038276266111184
3	0.004981235459603879
4	0.014830496482002456
5	0.033736549249135356
6	0.04415185975557983
7	0.06554853025251468
8	0.08660557105902199
9	0.09611520239099301
10	0.11796471247516459
11	0.11921002134006554
12	0.13222915947312114
13	0.12147421927624913
14	0.12294594793476846
15	0.11434199577727085
16	0.11388915619003413
17	0.12260631824434093
18	0.12815360318799068
19	0.1297385417433192
20	0.12147421927624913
21	0.12679508442628054
22	0.14853138461364293
23	0.1374368147263434
24	0.1339273079252588
25	0.12951212194970085
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	883315.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.34987832255402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.73747073810723	34.13259775922606
2	13.021330626785696	11.28946196537812
3	3.7289974725909247	4.849547601057842
4	1.5114697064926912	2.6208811145873843
5	0.741738377759265	1.6077134211516373
6	0.4287818794473927	1.11525853805763
7	0.30175065008536683	0.9156597775466558
8	0.22000820622699496	0.7629863175922882
9	0.16261110555792535	0.6344254475848846
>10	0.9178511550459724	7.612475157897082
>50	0.11517033122621309	3.5450984801068417
>100	0.09401645889540976	7.998595452178142
>500	0.007051234417115671	1.9919505036042642
>1k	0.00940164588948756	8.444421032329757
>5k	0.00156694098158126	4.852004244584913
>10k+	7.8347049079063E-4	7.626923187116548
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	30541	3.4575434584491376	No Hit
TCCATGTACTCTGCGTTGATACCAC	25719	2.911645336035276	No Hit
GAGTACATGGAAGCAGTGGTATCAA	11109	1.2576487436531703	No Hit
CATGTACTCTGCGTTGATACCACTG	9028	1.0220589483932685	No Hit
GCTTCCATGTACTCTGCGTTGATAC	7238	0.8194132331048379	No Hit
CCCATGTACTCTGCGTTGATACCAC	7120	0.8060544652813549	No Hit
GCGTTGATACCACTGCTTCCATGTA	6762	0.7655253222236689	No Hit
GTACATGGGAAGCAGTGGTATCAAC	6359	0.7199017338095697	No Hit
CATGGAAGCAGTGGTATCAACGCAG	6351	0.7189960546350962	No Hit
GTATCAACGCAGAGTACATGGAAGC	4959	0.5614078782767189	No Hit
CAGTGGTATCAACGCAGAGTACATG	4024	0.45555662476013653	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3911	0.4427639064206993	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	3744	0.4238578536535664	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3678	0.4163860004641606	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3546	0.4014422940853489	No Hit
TATCAACGCAGAGTACATGGAAGCA	3054	0.34574302485523284	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2875	0.3254784533263898	No Hit
GTATCAACGCAGAGTACATGGGAAG	2346	0.2655904179143341	No Hit
GGTATCAACGCAGAGTACATGGAAG	2191	0.2480428839089113	No Hit
ATACCACTGCTTCCATGTACTCTGC	2136	0.24181633958440646	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2128	0.24091066040993303	No Hit
GTACTCTGCGTTGATACCACTGCTT	2093	0.23694831402161176	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1940	0.21962719980980738	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1872	0.2119289268267832	No Hit
GTGGTATCAACGCAGAGTACATGGA	1811	0.20502312312142326	No Hit
GCGTTGATACCACTGCTTCCCATGT	1803	0.20411744394694983	No Hit
CCACTGCTTCCATGTACTCTGCGTT	1767	0.2000418876618194	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	1749	0.19800410951925418	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1727	0.19551349178945224	No Hit
ACATGGAAGCAGTGGTATCAACGCA	1631	0.18464534169577104	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1591	0.18011694582340387	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	1521	0.17219225304676136	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1508	0.170720524388242	No Hit
GTACATGGGGTGGTATCAACGCAAA	1488	0.16845632645205844	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	1487	0.16834311655524925	No Hit
TATCAACGCAGAGTACATGGGAAGC	1409	0.1595127446041333	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1289	0.1459275569870318	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	1274	0.14422940853489413	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1260	0.14264446997956562	No Hit
GTTGATACCACTGCTTCCATGTACT	1249	0.14139916111466463	No Hit
GATACCACTGCTTCCATGTACTCTG	1158	0.13109706050502937	No Hit
GGTATCAACGCAGAGTACATGGGAA	1149	0.13007817143374675	No Hit
CCCCATGTACTCTGCGTTGATACCA	1089	0.123285577625196	No Hit
CTTCCATGTACTCTGCGTTGATACC	1067	0.12079495989539406	No Hit
GCAGTGGTATCAACGCAGAGTACAT	1066	0.12068174999858487	No Hit
CCATGTACTCTGCGTTGATACCACT	998	0.11298347701556069	No Hit
GTACATGGGTGGTATCAACGCAAAA	928	0.10505878423891817	No Hit
ATCAACGCAGAGTACATGGAAGCAG	895	0.10132285764421527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1320989680917906E-4
2	0.0	0.0	0.0	0.0	1.1320989680917906E-4
3	0.0	0.0	0.0	0.0	1.1320989680917906E-4
4	0.0	0.0	0.0	0.0	1.1320989680917906E-4
5	0.0	0.0	0.0	0.0	1.1320989680917906E-4
6	0.0	0.0	0.0	0.0	1.1320989680917906E-4
7	0.0	0.0	0.0	0.0	1.1320989680917906E-4
8	0.0	0.0	0.0	0.0	1.1320989680917906E-4
9	0.0	0.0	0.0	1.1320989680917906E-4	1.1320989680917906E-4
10	1.1320989680917906E-4	0.0	0.0	3.396296904275372E-4	1.1320989680917906E-4
11	1.1320989680917906E-4	0.0	0.0	3.396296904275372E-4	1.1320989680917906E-4
12	1.1320989680917906E-4	0.0	0.0	3.396296904275372E-4	3.396296904275372E-4
13	1.1320989680917906E-4	0.0	0.0	3.396296904275372E-4	3.396296904275372E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGGT	70	4.444355E-7	14.924206	4
CATCGCC	65	3.364481E-6	14.616911	15
TTAGGAC	145	0.0	14.407946	3
GCATCGC	60	2.5632486E-5	14.25149	14
CTAGGGC	40	0.005290932	14.244219	3
CGTCAAT	75	9.635351E-7	13.93637	19
ATACCAA	190	0.0	13.502942	19
ATCAACC	185	0.0	13.35426	19
AACCGCG	50	0.0014974712	13.30139	7
ATCGCCA	80	1.9929957E-6	13.064606	16
CGACCAT	110	2.732122E-9	12.956634	10
GTGCTAG	60	4.1127478E-4	12.657942	1
GCGTAGG	75	1.4873689E-5	12.657941	1
TACGCTA	130	1.9645086E-10	12.425079	9
ATACGCT	130	1.9645086E-10	12.424375	8
AAAAAGT	345	0.0	12.382769	1
TCCCGGA	70	1.08823035E-4	12.216254	17
GGTTCCG	70	1.0888067E-4	12.215562	8
CGGTCCA	110	3.7965947E-8	12.092857	10
AAGACTG	55	0.0030694474	12.089431	5
>>END_MODULE