FastQCFastQC Report
Thu 2 Feb 2017
SRR4062365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883315
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA109311.2374973820211363No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC98551.1156835330544594No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA35790.4051782206800519No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC31680.35864895309147926No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG25780.2918551139740636No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC23030.2607223923515394No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA22280.2522316500908509No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21930.24826930370252967No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG21640.24498621669506349No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20260.22936325093539678No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA18140.20536275281185082No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA16060.18181509427554157No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA13410.15181447162110911No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13140.14875780440726127No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA12990.14705965595512357No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG12790.14479545801894003No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC12030.1361915058614424No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG11920.13494619699654142No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA11550.1307574308146018No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10680.12090816979220323No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA8850.10019075867612347No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG250.002352525735.1997743
TCGTATA2000.030.799844
CGTAACC300.005744366429.33314710
CACCGTA300.005744366429.3331477
CTAGGCG300.005744366429.33314738
GTCGTAT2300.026.78243643
CTACTAG508.3264626E-526.4058071
CTCGTAT1001.7444108E-924.19984443
CGCAATA1950.023.69215636
CTATGCG3900.023.12805738
TATGCGT3150.023.04747239
ATACGAA2050.022.5364440
AAGCGCT703.2142492E-521.9998597
TGGTATA7150.021.53832244
GCGCAAT2250.021.51097335
TCGTATC1158.763891E-921.04334344
TAGCGGC2200.020.99986530
CAATACG2200.020.99986538
AGTCGTA2350.020.59561342
TACGAAT2250.020.53320141
Produced by FastQC (version 0.11.5)