##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062365_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	883315
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.033533903533847	32.0	32.0	32.0	32.0	32.0
2	31.418808692255876	32.0	32.0	32.0	32.0	32.0
3	31.496023502374577	32.0	32.0	32.0	32.0	32.0
4	31.618010562483374	32.0	32.0	32.0	32.0	32.0
5	31.551142004834063	32.0	32.0	32.0	32.0	32.0
6	35.1507469022942	36.0	36.0	36.0	36.0	36.0
7	35.1678800880773	36.0	36.0	36.0	36.0	36.0
8	35.13921081380934	36.0	36.0	36.0	36.0	36.0
9	35.21939964791722	36.0	36.0	36.0	36.0	36.0
10	35.131732168026126	36.0	36.0	36.0	36.0	36.0
11	35.208603952157496	36.0	36.0	36.0	36.0	36.0
12	35.11807679027301	36.0	36.0	36.0	36.0	36.0
13	35.136775668928976	36.0	36.0	36.0	36.0	36.0
14	35.080642805794085	36.0	36.0	36.0	36.0	36.0
15	35.05480491104532	36.0	36.0	36.0	36.0	36.0
16	35.021041191420956	36.0	36.0	36.0	36.0	36.0
17	35.00134719777203	36.0	36.0	36.0	36.0	36.0
18	34.989864317938675	36.0	36.0	36.0	36.0	36.0
19	34.94197539948942	36.0	36.0	36.0	36.0	36.0
20	34.925411659487274	36.0	36.0	36.0	36.0	36.0
21	34.822780095435945	36.0	36.0	36.0	32.0	36.0
22	34.76877444626209	36.0	36.0	36.0	32.0	36.0
23	34.61201496634836	36.0	36.0	36.0	32.0	36.0
24	34.57040466877614	36.0	36.0	36.0	32.0	36.0
25	34.5233919949282	36.0	36.0	36.0	32.0	36.0
26	34.40433367484985	36.0	36.0	36.0	32.0	36.0
27	34.31764998896203	36.0	36.0	36.0	32.0	36.0
28	34.21230025528832	36.0	36.0	36.0	32.0	36.0
29	34.15973237180394	36.0	36.0	36.0	32.0	36.0
30	34.102211555334165	36.0	36.0	36.0	32.0	36.0
31	34.046396812009306	36.0	36.0	36.0	32.0	36.0
32	33.96644458658576	36.0	36.0	36.0	32.0	36.0
33	33.874637020768354	36.0	36.0	36.0	32.0	36.0
34	33.802577789350345	36.0	36.0	36.0	32.0	36.0
35	33.61272479240135	36.0	36.0	36.0	27.0	36.0
36	33.56426303187425	36.0	36.0	36.0	27.0	36.0
37	33.36681704714626	36.0	36.0	36.0	21.0	36.0
38	33.24343524110878	36.0	36.0	36.0	14.0	36.0
39	32.82035174314939	36.0	36.0	36.0	14.0	36.0
40	32.556907784878554	36.0	36.0	36.0	14.0	36.0
41	32.641377085184786	36.0	36.0	36.0	14.0	36.0
42	32.19709276984994	36.0	36.0	36.0	14.0	36.0
43	32.38510610597579	36.0	36.0	36.0	14.0	36.0
44	32.377881050361424	36.0	36.0	36.0	14.0	36.0
45	31.891452086741424	36.0	36.0	36.0	14.0	36.0
46	32.30539614973141	36.0	36.0	36.0	14.0	36.0
47	32.003412146289826	36.0	36.0	36.0	14.0	36.0
48	32.16125164861912	36.0	36.0	36.0	14.0	36.0
49	32.324550132172554	36.0	36.0	36.0	14.0	36.0
50	31.596207468456893	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	7.0
21	51.0
22	190.0
23	549.0
24	1595.0
25	4153.0
26	9136.0
27	17412.0
28	26473.0
29	33499.0
30	40945.0
31	51212.0
32	72045.0
33	72682.0
34	132192.0
35	421173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.30879001056418	16.55802430554376	13.733775828744765	24.39940985514729
2	13.070610118794908	21.9320560587895	36.17057649438971	28.826757328025888
3	19.19120219265921	27.43076900434841	27.17898905368551	26.19903974930687
4	12.576487436531703	17.81131306498814	33.011213440278944	36.60098605820121
5	13.02366652892796	37.761047870804866	33.71820924585227	15.497076354414904
6	34.54253782372626	31.802270555683858	18.57755165808529	15.077639962504586
7	28.164584547981185	30.798752426937163	22.787793708926035	18.24886931615562
8	29.911639675540435	31.368311417784145	21.321499125453546	17.398549781221874
9	27.179998075431755	16.382943796946726	17.44621114777854	38.99084697984298
10	15.863197160695789	28.01933625037501	32.31282158686312	23.80464500206608
11	36.068559913507634	21.702450428216437	19.352892229838734	22.876097428437195
12	22.609488121451577	24.3586942370502	29.581066776857632	23.450750864640586
13	32.8737766255526	20.1751357103638	23.910496255582665	23.040591408500934
14	26.038389476007996	18.331965380413557	23.800909075471377	31.828736068107073
15	27.597289755070385	24.158991979078813	26.770291458879335	21.473426806971467
16	26.97554100179438	24.194313466883273	26.315414093500056	22.514731437822295
17	24.54933970327686	24.204275937802482	28.84633454656606	22.4000498123546
18	27.592648149301212	21.1223629169662	30.082586619722296	21.20240231401029
19	23.551620882697566	30.806677119713804	24.66379490895094	20.97790708863769
20	23.314106519191906	21.709129812128175	32.609884356090404	22.366879312589507
21	24.37578893146839	27.16935634513169	22.390540181022626	26.064314542377293
22	22.594544415072765	27.040749902356463	24.49964055857763	25.86506512399314
23	21.856868727464153	25.725137691536993	24.023026893010986	28.394966687987868
24	21.480219400780015	29.394157237225677	24.370694486111976	24.754928875882328
25	26.623231802924213	23.135348092130215	22.74318900958322	27.498231095362357
26	21.650487085581023	23.64717003560451	26.428171150721997	28.27417172809247
27	27.258792163610945	26.32288594668946	25.08470930528747	21.33361258441213
28	24.905724458432157	21.585617814709362	25.780950170663917	27.727707556194563
29	22.167403474411735	26.723422561600334	29.02396087465966	22.085213089328267
30	27.2471315442396	23.072403389504313	27.51577862936778	22.16468643688831
31	21.757357228168885	24.602774774570793	26.263111121174216	27.376756876086105
32	22.215404470658825	26.31609335288091	26.182505674646077	25.28599650181419
33	21.95173862099025	25.31226119787392	26.299451498049958	26.436548683085874
34	21.211572315651832	27.350039340439142	28.72078477100468	22.717603572904345
35	26.020049472724903	24.893384579679957	26.538777219904564	22.547788727690573
36	24.18695482359068	26.38096262375257	26.598778465213428	22.833304087443324
37	25.929594765174375	26.227789633369746	26.624250691995492	21.218364909460384
38	24.011705903330068	26.1260139361383	23.682265103615357	26.18001505691628
39	22.835002235895463	25.763289426761688	27.37505872763397	24.02664960970888
40	25.35075256278904	25.805063878684276	24.055178503704795	24.789005054821892
41	21.56093805720496	25.713024232578412	27.096675591380198	25.62936211883643
42	27.075392130780074	25.8251020304195	25.896990314893326	21.2025155239071
43	25.49475555153031	22.721226289602235	24.158991979078813	27.625026179788637
44	24.520018340003283	24.08178282945495	29.14849176114976	22.249707069392006
45	25.856008332248404	24.7274188709577	25.88476364603794	23.531809150755958
46	23.162344491703394	24.171046956175218	29.266975579437865	23.399632972683523
47	25.405235284394905	23.005862015093157	29.111618292022996	22.47728440848894
48	22.713528016619215	31.084154576793104	23.55535680929227	22.646960597295415
49	21.756111919303986	24.646813424429563	31.10498519780599	22.49208945846046
50	21.589353741304066	30.70705241052173	22.31401029077962	25.389583557394584
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13.0
1	22.5
2	32.0
3	156.0
4	280.0
5	307.0
6	334.0
7	321.5
8	309.0
9	406.0
10	503.0
11	776.0
12	1049.0
13	1765.5
14	2482.0
15	3305.0
16	4128.0
17	4683.5
18	5239.0
19	5598.0
20	5957.0
21	6357.5
22	6758.0
23	6786.0
24	6814.0
25	6241.5
26	5669.0
27	6193.0
28	6717.0
29	7829.5
30	8942.0
31	10399.0
32	11856.0
33	14097.0
34	16338.0
35	18483.0
36	20628.0
37	23232.5
38	25837.0
39	28427.0
40	31017.0
41	35962.5
42	40908.0
43	48956.5
44	57005.0
45	72819.5
46	88634.0
47	99630.5
48	110627.0
49	99846.5
50	89066.0
51	74064.0
52	59062.0
53	52826.0
54	46590.0
55	46608.0
56	46626.0
57	45218.0
58	43810.0
59	40348.0
60	36886.0
61	32750.5
62	28615.0
63	25086.5
64	21558.0
65	18640.5
66	15723.0
67	13729.5
68	11736.0
69	10424.5
70	9113.0
71	7515.0
72	5917.0
73	5175.5
74	4434.0
75	3193.5
76	1953.0
77	1753.0
78	1553.0
79	1312.5
80	1072.0
81	872.0
82	672.0
83	563.5
84	455.0
85	347.5
86	240.0
87	165.0
88	90.0
89	64.5
90	39.0
91	27.5
92	16.0
93	10.5
94	5.0
95	3.5
96	2.0
97	3.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016075805346903425
2	0.0031698771106570137
3	4.5283958723671625E-4
4	0.0
5	0.0
6	7.924692776642534E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.2641979361835813E-4
47	1.1320989680917906E-4
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	883315.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.49386041567581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.40107180053087	43.00963712168328
2	11.838436759281112	12.665673670815785
3	3.4385277156032172	5.518203649617333
4	1.4021857289910682	3.0003331065400336
5	0.750534626011802	2.0074497260503392
6	0.4361405308685895	1.3998504407941845
7	0.26803925597717215	1.0036918181615113
8	0.20236229133002545	0.8660112132643765
9	0.1501275227866969	0.7227810673652477
>10	0.9172589152028612	9.362353861771462
>50	0.10341535362445038	3.932785685348116
>100	0.08123830492913979	8.190884310024316
>500	0.006396716917800165	2.1272505925989
>1k	0.0038380301506800987	3.8222073650396315
>5k	2.132238972600055E-4	1.1240779941052095
>10k+	2.132238972600055E-4	1.2468083768202987
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	10931	1.2374973820211363	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	9855	1.1156835330544594	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	3579	0.4051782206800519	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	3168	0.35864895309147926	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	2578	0.2918551139740636	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	2303	0.2607223923515394	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	2228	0.2522316500908509	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2193	0.24826930370252967	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	2164	0.24498621669506349	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2026	0.22936325093539678	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1814	0.20536275281185082	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	1606	0.18181509427554157	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	1341	0.15181447162110911	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1314	0.14875780440726127	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA	1299	0.14705965595512357	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	1279	0.14479545801894003	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	1203	0.1361915058614424	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1192	0.13494619699654142	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	1155	0.1307574308146018	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	1068	0.12090816979220323	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	885	0.10019075867612347	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1320989680917906E-4
2	0.0	0.0	0.0	0.0	1.1320989680917906E-4
3	0.0	0.0	0.0	0.0	1.1320989680917906E-4
4	0.0	0.0	0.0	0.0	1.1320989680917906E-4
5	0.0	0.0	0.0	0.0	1.1320989680917906E-4
6	0.0	0.0	0.0	0.0	1.1320989680917906E-4
7	0.0	0.0	0.0	0.0	1.1320989680917906E-4
8	0.0	0.0	0.0	0.0	1.1320989680917906E-4
9	0.0	0.0	0.0	1.1320989680917906E-4	1.1320989680917906E-4
10	1.1320989680917906E-4	0.0	0.0	2.2641979361835813E-4	1.1320989680917906E-4
11	1.1320989680917906E-4	0.0	0.0	2.2641979361835813E-4	1.1320989680917906E-4
12	1.1320989680917906E-4	0.0	0.0	2.2641979361835813E-4	1.1320989680917906E-4
13	1.1320989680917906E-4	0.0	0.0	3.396296904275372E-4	1.1320989680917906E-4
14	1.1320989680917906E-4	0.0	0.0	4.5283958723671625E-4	1.1320989680917906E-4
15	1.1320989680917906E-4	0.0	0.0	7.924692776642534E-4	1.1320989680917906E-4
16	1.1320989680917906E-4	0.0	0.0	0.0013585187617101487	1.1320989680917906E-4
17	1.1320989680917906E-4	0.0	0.0	0.0015849385553285068	1.1320989680917906E-4
18	1.1320989680917906E-4	0.0	0.0	0.0016981484521376858	1.1320989680917906E-4
19	1.1320989680917906E-4	0.0	0.0	0.002150988039374402	1.1320989680917906E-4
20	1.1320989680917906E-4	0.0	0.0	0.0026038276266111184	2.2641979361835813E-4
21	1.1320989680917906E-4	0.0	0.0	0.004301976078748804	3.396296904275372E-4
22	1.1320989680917906E-4	0.0	0.0	0.007811482879833355	3.396296904275372E-4
23	1.1320989680917906E-4	0.0	0.0	0.012226668855391339	3.396296904275372E-4
24	1.1320989680917906E-4	0.0	0.0	0.01800037359265947	3.396296904275372E-4
25	1.1320989680917906E-4	0.0	0.0	0.02275518925864499	3.396296904275372E-4
26	1.1320989680917906E-4	0.0	0.0	0.028528893995913122	3.396296904275372E-4
27	1.1320989680917906E-4	0.0	0.0	0.037812105534265804	3.396296904275372E-4
28	1.1320989680917906E-4	0.0	0.0	0.04958593480242043	3.396296904275372E-4
29	1.1320989680917906E-4	0.0	0.0	0.06747309849827073	3.396296904275372E-4
30	1.1320989680917906E-4	0.0	0.0	0.09215285600267176	3.396296904275372E-4
31	2.2641979361835813E-4	0.0	0.0	0.1449086679157492	3.396296904275372E-4
32	2.2641979361835813E-4	0.0	0.0	0.20841942002569866	3.396296904275372E-4
33	2.2641979361835813E-4	0.0	0.0	0.2643451090494331	3.396296904275372E-4
34	2.2641979361835813E-4	0.0	0.0	0.34664870402970627	3.396296904275372E-4
35	3.396296904275372E-4	0.0	0.0	0.43110328704935386	3.396296904275372E-4
36	3.396296904275372E-4	0.0	0.0	0.5571059021979702	3.396296904275372E-4
37	3.396296904275372E-4	0.0	0.0	0.7535250731618958	3.396296904275372E-4
38	3.396296904275372E-4	0.0	0.0	0.9422459711427973	3.396296904275372E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	25	0.0023525257	35.19977	43
TCGTATA	200	0.0	30.7998	44
CGTAACC	30	0.0057443664	29.333147	10
CACCGTA	30	0.0057443664	29.333147	7
CTAGGCG	30	0.0057443664	29.333147	38
GTCGTAT	230	0.0	26.782436	43
CTACTAG	50	8.3264626E-5	26.405807	1
CTCGTAT	100	1.7444108E-9	24.199844	43
CGCAATA	195	0.0	23.692156	36
CTATGCG	390	0.0	23.128057	38
TATGCGT	315	0.0	23.047472	39
ATACGAA	205	0.0	22.53644	40
AAGCGCT	70	3.2142492E-5	21.999859	7
TGGTATA	715	0.0	21.538322	44
GCGCAAT	225	0.0	21.510973	35
TCGTATC	115	8.763891E-9	21.043343	44
TAGCGGC	220	0.0	20.999865	30
CAATACG	220	0.0	20.999865	38
AGTCGTA	235	0.0	20.595613	42
TACGAAT	225	0.0	20.533201	41
>>END_MODULE