FastQCFastQC Report
Thu 2 Feb 2017
SRR4062364_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062364_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2659322
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG74030.2783792259831641No Hit
TCCATGTACTCTGCGTTGATACCAC63940.2404372242248212No Hit
GTATCAACGCAGAGTACTTTTTTTT52630.19790758697141603No Hit
TATCAACGCAGAGTACTTTTTTTTT35260.1325901865212261No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG33770.12698725464610905No Hit
GTACTTTTTTTTTTTTTTTTTTTTT33450.12578394041789598No Hit
GTCTTGCGCCGGTCCAAGAATTTCA33130.12458062618968295No Hit
GAATAGGACCGCGGTTCTATTTTGT32290.12142192634062365No Hit
CTATTGGAGCTGGAATTACCGCGGC30850.1160070123136649No Hit
GATTAAGAGGGACGGCCGGGGGCAT30830.11593180517440159No Hit
GTATCTGATCGTCTTCGAACCTCCG30290.11390121241429206No Hit
GCGCAAGACGGACCAGAGCGAAAGC29400.1105544947170745No Hit
GAACTACGACGGTATCTGATCGTCT29300.11017845902075792No Hit
GAGTACATGGAAGCAGTGGTATCAA28790.10826067696954338No Hit
GTCCTATTCCATTATTCCTAGCTGC28540.10732058772875191No Hit
GGGTAGGCACACGCTGAGCCAGTCA28520.10724538058948858No Hit
GAATAACGCCGCCGCATCGCCAGTC27200.10228170939810975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG1257.2759576E-1213.68141113
AATCGTA1500.013.30137213
CGCTTAC1153.765308E-1013.22124418
TCGACGC1153.765308E-1013.21875414
CGCATCG5650.013.11639613
ATCGCCA5800.012.77886416
CCGCATC6000.012.66821212
GCATCGC5850.012.66797314
TCGCGTA2250.012.6677349
GCGTTAT2700.012.6581971
CGCCAGT5800.012.61574518
TCGCCAG5900.012.56274717
CGTTATT2800.012.5463592
CATCGCC5900.012.39981715
AACCGCG2850.012.33437257
ACGGTAT4350.012.2309169
ATTGGAC701.0952147E-412.2097963
CGACGCT1251.4097168E-912.16148315
CGCGTAA2350.012.1289110
GCGTTCC2350.012.1286819
Produced by FastQC (version 0.11.5)