##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062364_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2659322
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.167666044202242	32.0	32.0	32.0	32.0	32.0
2	30.805544044685075	32.0	32.0	32.0	32.0	32.0
3	30.79962524282505	32.0	32.0	32.0	32.0	32.0
4	30.87429277086415	32.0	32.0	32.0	32.0	32.0
5	30.637098478484365	32.0	32.0	32.0	32.0	32.0
6	34.305242840092326	36.0	36.0	36.0	32.0	36.0
7	34.17158508822926	36.0	36.0	36.0	32.0	36.0
8	34.16499769490118	36.0	36.0	36.0	32.0	36.0
9	34.34588214590035	36.0	36.0	36.0	32.0	36.0
10	33.98656875699896	36.0	36.0	36.0	32.0	36.0
11	34.33560847464128	36.0	36.0	36.0	32.0	36.0
12	34.08287977161096	36.0	36.0	36.0	32.0	36.0
13	34.23496966520038	36.0	36.0	36.0	32.0	36.0
14	34.12313552100874	36.0	36.0	36.0	32.0	36.0
15	34.049730720837864	36.0	36.0	36.0	32.0	36.0
16	34.05286610647376	36.0	36.0	36.0	32.0	36.0
17	33.965786392170635	36.0	36.0	36.0	32.0	36.0
18	33.98909383669973	36.0	36.0	36.0	32.0	36.0
19	33.97760368996308	36.0	36.0	36.0	32.0	36.0
20	33.96128524488573	36.0	36.0	36.0	32.0	36.0
21	33.93281934267456	36.0	36.0	36.0	32.0	36.0
22	33.87747027249803	36.0	36.0	36.0	32.0	36.0
23	33.84147951996787	36.0	36.0	36.0	32.0	36.0
24	33.80694327351107	36.0	36.0	36.0	32.0	36.0
25	33.338750628919705	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	21.0
4	105.0
5	237.0
6	465.0
7	120.0
8	387.0
9	445.0
10	305.0
11	98.0
12	208.0
13	136.0
14	432.0
15	559.0
16	908.0
17	1301.0
18	1777.0
19	2527.0
20	3771.0
21	5307.0
22	7817.0
23	11496.0
24	15843.0
25	22398.0
26	31460.0
27	41025.0
28	56156.0
29	75464.0
30	98990.0
31	135944.0
32	197009.0
33	282912.0
34	614339.0
35	1049357.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86939817901684	17.65257813413721	11.692282351295338	25.785741335550615
2	16.73521405248359	20.20693200402673	37.221437325301935	25.83641661818774
3	18.922491435361895	24.436087680835143	27.998488261463077	28.642932622339885
4	12.398737233557634	16.00455673046691	36.2199521310124	35.37675390496306
5	14.5418098144624	36.80594556916742	33.411588924545484	15.240655691824692
6	33.46776514511676	35.513774683505254	17.595757271826766	13.42270289955122
7	30.316445479315355	30.6766643424717	20.86767102493988	18.13921915327307
8	28.255922836397684	32.01120510168596	19.946827328826	19.786044733090353
9	27.462861169201307	15.190448014670347	18.84832361483114	38.49836720129721
10	16.057465876812042	26.448233086174344	31.11838972059552	26.375911316418094
11	36.87710780596928	21.720895806713205	22.2144960578993	19.187500329418214
12	25.039300228139	24.216508769175217	28.20425349899109	22.53993750369469
13	28.938094944076575	19.752105693542337	25.540673430386747	25.769125931994342
14	23.813252923594057	20.325534447036837	24.983368240888865	30.87784438848024
15	25.42251328630313	27.029330570600465	22.718684248469774	24.829471894626636
16	26.025948172921566	25.9559655048314	23.821362369427174	24.196723952819863
17	24.0377230341072	25.79225299085575	25.554460380950083	24.61556359408696
18	24.9789145194619	24.823898584863034	26.571470742389646	23.62571615328542
19	25.26173500989331	25.73966379688497	25.46351712361649	23.535084069605226
20	25.36735098796699	24.303012938841785	25.52129786744605	24.808338205745166
21	26.728049869647926	24.573889522283352	24.224928498383214	24.473132109685515
22	25.382502085416398	24.951108048106743	24.924444846149935	24.741945020326924
23	23.804142643958325	24.62074213929788	25.675889905548438	25.89922531119535
24	24.17258179482265	25.75333397847171	25.153844792509567	24.920239434196073
25	24.54939096711034	24.86940150987443	25.235273076416213	25.345934446599017
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	361.0
1	361.0
2	927.5
3	1494.0
4	1494.0
5	1494.0
6	3868.0
7	6242.0
8	6242.0
9	6242.0
10	6190.0
11	6138.0
12	6138.0
13	6138.0
14	7239.0
15	8340.0
16	8340.0
17	8340.0
18	13285.5
19	18231.0
20	18231.0
21	18231.0
22	30287.5
23	42344.0
24	42344.0
25	42344.0
26	63866.5
27	85389.0
28	85389.0
29	85389.0
30	104894.5
31	124400.0
32	124400.0
33	124400.0
34	153201.0
35	182002.0
36	182002.0
37	182002.0
38	201677.5
39	221353.0
40	221353.0
41	221353.0
42	248723.0
43	276093.0
44	276093.0
45	276093.0
46	321593.5
47	367094.0
48	367094.0
49	367094.0
50	354433.0
51	341772.0
52	341772.0
53	341772.0
54	310439.0
55	279106.0
56	279106.0
57	279106.0
58	256578.5
59	234051.0
60	234051.0
61	234051.0
62	208619.0
63	183187.0
64	183187.0
65	183187.0
66	154248.5
67	125310.0
68	125310.0
69	125310.0
70	96247.0
71	67184.0
72	67184.0
73	67184.0
74	54345.5
75	41507.0
76	41507.0
77	41507.0
78	34382.0
79	27257.0
80	27257.0
81	27257.0
82	19579.0
83	11901.0
84	11901.0
85	11901.0
86	8682.5
87	5464.0
88	5464.0
89	5464.0
90	3649.0
91	1834.0
92	1834.0
93	1834.0
94	1052.5
95	271.0
96	271.0
97	271.0
98	634.0
99	997.0
100	997.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007859146053016521
2	0.0034219248364808774
3	0.004963671191378855
4	0.014966220713399881
5	0.035309751884126855
6	0.04591395852025441
7	0.06659592181766631
8	0.08716507440618323
9	0.09528744544662136
10	0.11796239793451112
11	0.11747355152929957
12	0.1310484401663281
13	0.119955387124989
14	0.12255003342957341
15	0.11337476243944884
16	0.11093053041339108
17	0.12130911563172869
18	0.1303339723433266
19	0.13112364730559142
20	0.12074506208725382
21	0.12928107239364017
22	0.14954939642510384
23	0.13819311839634313
24	0.13390631145833412
25	0.13055959376111653
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2659322.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.87803361344894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.40791860729678	32.648731537577405
2	14.7672781499215	12.959182540828113
3	4.9660856366888915	6.537062174817035
4	2.1480478662538935	3.7700846591514243
5	1.0905520742799002	2.3925640286234966
6	0.6292767458489114	1.656685572391218
7	0.39523948654034474	1.2139632033045684
8	0.24832513159886443	0.8716814777087285
9	0.18941058520467935	0.7479867621918559
>10	0.9013256477335088	7.189897737871174
>50	0.10268796434502142	3.201386615489912
>100	0.12129278488540603	11.42662595808464
>500	0.020563780675300247	6.294507851121953
>1k	0.011738491468817015	8.373064679601168
>5k	2.570472584412485E-4	0.7165752012374592
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	7403	0.2783792259831641	No Hit
TCCATGTACTCTGCGTTGATACCAC	6394	0.2404372242248212	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5263	0.19790758697141603	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3526	0.1325901865212261	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3377	0.12698725464610905	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3345	0.12578394041789598	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	3313	0.12458062618968295	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3229	0.12142192634062365	No Hit
CTATTGGAGCTGGAATTACCGCGGC	3085	0.1160070123136649	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3083	0.11593180517440159	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3029	0.11390121241429206	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2940	0.1105544947170745	No Hit
GAACTACGACGGTATCTGATCGTCT	2930	0.11017845902075792	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2879	0.10826067696954338	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2854	0.10732058772875191	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2852	0.10724538058948858	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2720	0.10228170939810975	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.7603569631658E-5	0.0
5	0.0	0.0	0.0	3.7603569631658E-5	0.0
6	0.0	0.0	0.0	3.7603569631658E-5	3.7603569631658E-5
7	0.0	0.0	0.0	7.5207139263316E-5	3.7603569631658E-5
8	0.0	0.0	0.0	1.1281070889497398E-4	3.7603569631658E-5
9	0.0	0.0	0.0	1.50414278526632E-4	3.7603569631658E-5
10	0.0	0.0	0.0	1.50414278526632E-4	3.7603569631658E-5
11	0.0	0.0	0.0	1.8801784815828998E-4	3.7603569631658E-5
12	0.0	0.0	0.0	1.8801784815828998E-4	7.5207139263316E-5
13	0.0	0.0	0.0	2.2562141778994797E-4	1.1281070889497398E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGACG	125	7.2759576E-12	13.681411	13
AATCGTA	150	0.0	13.301372	13
CGCTTAC	115	3.765308E-10	13.221244	18
TCGACGC	115	3.765308E-10	13.218754	14
CGCATCG	565	0.0	13.116396	13
ATCGCCA	580	0.0	12.778864	16
CCGCATC	600	0.0	12.668212	12
GCATCGC	585	0.0	12.667973	14
TCGCGTA	225	0.0	12.667734	9
GCGTTAT	270	0.0	12.658197	1
CGCCAGT	580	0.0	12.615745	18
TCGCCAG	590	0.0	12.562747	17
CGTTATT	280	0.0	12.546359	2
CATCGCC	590	0.0	12.399817	15
AACCGCG	285	0.0	12.3343725	7
ACGGTAT	435	0.0	12.230916	9
ATTGGAC	70	1.0952147E-4	12.209796	3
CGACGCT	125	1.4097168E-9	12.161483	15
CGCGTAA	235	0.0	12.12891	10
GCGTTCC	235	0.0	12.128681	9
>>END_MODULE