FastQCFastQC Report
Thu 2 Feb 2017
SRR4062364_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062364_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2659322
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC35270.13262779009085773No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT33400.1255959225697377No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA31990.12029381925167393No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA31160.1171727229722463No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA29190.10976481975480969No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT28080.10559082352569564No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC28010.10532759853827404No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC27790.10450032000637757No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA27670.10404907717079766No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG27390.10299617722111123No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA27110.10194327727142483No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT27060.10175525942326653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA9400.019.6594436
GGCGTTA8100.018.4690142
CAATACG10400.017.7691138
CGTCGTA8600.017.65104310
ATACCGT9100.017.648236
TAGTACG2800.017.2865722
ATACGAA10600.017.226340
CCGTCGT9200.016.9781449
TACCGTC9200.016.9781447
AATACGA10650.016.9388539
CGAATGC10550.016.89087943
CTAGCGG11150.016.77118529
TAGCGGC11300.016.74324830
CGGTCCA11350.016.66948910
ACCGTCG9300.016.5590278
CGCCGGT11250.016.4265547
TAGGACG4050.016.2961854
CGGCGTT9200.016.26075741
GCAATAC11400.016.21041737
TCTAGCG11550.016.19036528
Produced by FastQC (version 0.11.5)