##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062364_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2659322
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.210769135892534	32.0	32.0	32.0	32.0	32.0
2	31.313721316937173	32.0	32.0	32.0	32.0	32.0
3	31.439941082727103	32.0	32.0	32.0	32.0	32.0
4	31.568062837068997	32.0	32.0	32.0	32.0	32.0
5	31.464544722301397	32.0	32.0	32.0	32.0	32.0
6	35.06892132656369	36.0	36.0	36.0	36.0	36.0
7	35.08463247399149	36.0	36.0	36.0	36.0	36.0
8	35.018374608264814	36.0	36.0	36.0	36.0	36.0
9	35.17280908442076	36.0	36.0	36.0	36.0	36.0
10	35.0205296688404	36.0	36.0	36.0	36.0	36.0
11	35.1854040240332	36.0	36.0	36.0	36.0	36.0
12	35.078548592460784	36.0	36.0	36.0	36.0	36.0
13	35.12958528527196	36.0	36.0	36.0	36.0	36.0
14	35.09309026887304	36.0	36.0	36.0	36.0	36.0
15	35.04343550724583	36.0	36.0	36.0	36.0	36.0
16	35.05360276040284	36.0	36.0	36.0	36.0	36.0
17	35.02120540498669	36.0	36.0	36.0	36.0	36.0
18	35.04273570481499	36.0	36.0	36.0	36.0	36.0
19	35.017486035914416	36.0	36.0	36.0	36.0	36.0
20	35.017620280658	36.0	36.0	36.0	36.0	36.0
21	35.00368101343124	36.0	36.0	36.0	36.0	36.0
22	34.9802765516925	36.0	36.0	36.0	36.0	36.0
23	34.93353907499731	36.0	36.0	36.0	32.0	36.0
24	34.90805400775085	36.0	36.0	36.0	32.0	36.0
25	34.87439279635937	36.0	36.0	36.0	32.0	36.0
26	34.83302435733619	36.0	36.0	36.0	32.0	36.0
27	34.80986619897854	36.0	36.0	36.0	32.0	36.0
28	34.78183913042497	36.0	36.0	36.0	32.0	36.0
29	34.756339397786356	36.0	36.0	36.0	32.0	36.0
30	34.747530009528745	36.0	36.0	36.0	32.0	36.0
31	34.741311883254454	36.0	36.0	36.0	32.0	36.0
32	34.69916317016142	36.0	36.0	36.0	32.0	36.0
33	34.67294370520005	36.0	36.0	36.0	32.0	36.0
34	34.65343722948932	36.0	36.0	36.0	32.0	36.0
35	34.61642441193658	36.0	36.0	36.0	32.0	36.0
36	34.600252244745086	36.0	36.0	36.0	32.0	36.0
37	34.57888589648038	36.0	36.0	36.0	32.0	36.0
38	34.52622134513985	36.0	36.0	36.0	32.0	36.0
39	34.50491441051516	36.0	36.0	36.0	32.0	36.0
40	34.47975687036019	36.0	36.0	36.0	32.0	36.0
41	34.44149674240276	36.0	36.0	36.0	32.0	36.0
42	34.39589301333197	36.0	36.0	36.0	32.0	36.0
43	34.395598201346054	36.0	36.0	36.0	32.0	36.0
44	34.36283872355435	36.0	36.0	36.0	32.0	36.0
45	34.29167998459758	36.0	36.0	36.0	32.0	36.0
46	34.32230922016965	36.0	36.0	36.0	32.0	36.0
47	34.272127256496205	36.0	36.0	36.0	32.0	36.0
48	34.24196693743744	36.0	36.0	36.0	32.0	36.0
49	34.24148711588894	36.0	36.0	36.0	32.0	36.0
50	33.69222907192134	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	16.0
21	82.0
22	242.0
23	734.0
24	2005.0
25	4646.0
26	9801.0
27	18234.0
28	30047.0
29	44995.0
30	63810.0
31	92368.0
32	138502.0
33	224877.0
34	494656.0
35	1534304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.15453993957604	18.408284092657095	12.267575922033307	26.16960004573356
2	16.085512325235342	20.753537034745918	36.83825965443237	26.322690985586373
3	18.258318063041333	24.744829754262675	28.436022521690834	28.56082966100516
4	12.181924907786474	16.532026832453596	36.36249458037534	34.9235536793846
5	14.348694892908794	36.95708906255053	33.24486466851325	15.449351376027423
6	33.67678120653238	35.769469263984945	17.182183177797825	13.371566351684855
7	30.262744254537633	30.807846829786307	20.741536545487637	18.187872370188423
8	28.254317549479737	32.1600137779531	19.77651438092656	19.80915429164061
9	27.567252104107737	14.845212426325205	18.730977294212586	38.85655817535447
10	16.006636277968596	26.517736475688164	31.010498164569768	26.465129081773476
11	37.043163633437395	21.603589185514203	22.154519084187623	19.19872809686078
12	24.98806086664195	24.2455031771256	28.08847518277215	22.677960773460303
13	29.08049495322492	19.60646360237685	25.50198885279782	25.811052591600415
14	23.862360406148635	20.158596815278482	24.95745156096178	31.021591217611107
15	25.481645321627088	26.95175687637676	22.563909146767486	25.00268865522866
16	26.129253997823504	25.960037934481043	23.6558792053012	24.25482886239425
17	24.198160282959343	25.78717432488431	25.2951692198237	24.719496172332647
18	25.238989486794	24.653614718337984	26.49630244099812	23.611093353869897
19	25.47145475425691	25.576782352795185	25.428774702724983	23.52298819022292
20	25.4478021089586	24.208463661038415	25.47931390030993	24.864420329693058
21	26.85564215239824	24.4766523196514	24.250466848316975	24.41723867963338
22	25.49883015294876	24.800832693445923	24.89690981385481	24.80342733975051
23	23.887329176384057	24.452021981542664	25.624990129062976	26.035658713010307
24	24.22764148155056	25.63969312478895	25.086619822646522	25.046045571013963
25	24.722993304308392	24.69009018088069	25.235492354818255	25.351424159992657
26	24.167851805836225	25.495784263808595	25.852416518195238	24.483947412159942
27	25.55557393952293	25.008930847787518	24.757287759812463	24.67820745287709
28	24.212600053697898	24.781203630098197	25.980907915626617	25.02528840057729
29	24.154728160034775	25.236432444059048	25.897954441019177	24.710884954886996
30	24.54512841995065	25.07037508056565	26.252142463379762	24.13235403610394
31	24.813730717829582	25.033862014453305	24.830577117024568	25.32183015069254
32	24.46856755218059	25.080528044366197	25.018632568752487	25.432271834700725
33	24.106482780197357	24.571864557958758	25.576030281402552	25.74562238044133
34	24.609430524020784	25.124674635113763	25.9123566081881	24.35353823267735
35	25.845046218547434	24.750293495860976	25.302088276635924	24.102572008955665
36	24.27818067913551	25.263093374927898	25.430391656219143	25.028334289717453
37	25.825041119503396	25.106324093133516	24.761160927484525	24.30747385987857
38	24.403024530312614	24.887508921446898	25.514999687890374	25.19446686035012
39	24.852988844525033	25.062779159500053	24.824485338744235	25.259746657230675
40	25.21146367382363	25.150395476741817	25.423021356571336	24.21511949286322
41	23.997733256822602	25.529514665768193	25.83071925851777	24.642032818891433
42	25.80289261699035	25.500559917151815	25.134263545369834	23.562283920488003
43	24.705658058708195	24.32785499461893	25.39526992218317	25.5712170244897
44	24.512902160776317	24.984751752514363	25.30878171203036	25.19356437467896
45	24.60292510647451	25.34597164239607	25.362554816603634	24.688548434525792
46	24.39368876016755	24.819136026930202	25.564138788915507	25.223036423986745
47	24.592621728395432	24.683020709789936	25.914161579530422	24.810195982284206
48	25.36443499508521	25.817821234134115	24.60300031361377	24.2147434571669
49	24.073316431782235	25.425540795736655	25.571743474464544	24.92939929801656
50	24.02388580539386	25.97036084412557	25.01440217800039	24.991351172480183
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	31.0
2	43.0
3	127.5
4	212.0
5	267.0
6	322.0
7	325.0
8	328.0
9	429.5
10	531.0
11	817.5
12	1104.0
13	2083.0
14	3062.0
15	4116.0
16	5170.0
17	6337.5
18	7505.0
19	8298.5
20	9092.0
21	10458.5
22	11825.0
23	12761.0
24	13697.0
25	15975.0
26	18253.0
27	23286.0
28	28319.0
29	33252.0
30	38185.0
31	41713.5
32	45242.0
33	52176.0
34	59110.0
35	68702.0
36	78294.0
37	95180.5
38	112067.0
39	119912.0
40	127757.0
41	139511.5
42	151266.0
43	151683.5
44	152101.0
45	167258.5
46	182416.0
47	193865.5
48	205315.0
49	205530.0
50	205745.0
51	190096.5
52	174448.0
53	167654.5
54	160861.0
55	163989.5
56	167118.0
57	165813.5
58	164509.0
59	148334.5
60	132160.0
61	116223.5
62	100287.0
63	89663.5
64	79040.0
65	68143.0
66	57246.0
67	53365.0
68	49484.0
69	47385.5
70	45287.0
71	35251.0
72	25215.0
73	21888.5
74	18562.0
75	13695.5
76	8829.0
77	7838.0
78	6847.0
79	5989.0
80	5131.0
81	3923.0
82	2715.0
83	2486.5
84	2258.0
85	1817.5
86	1377.0
87	1023.0
88	669.0
89	432.5
90	196.0
91	127.0
92	58.0
93	40.5
94	23.0
95	15.5
96	8.0
97	10.0
98	12.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01665838134682449
2	0.003873167672060773
3	7.144678230015019E-4
4	1.8801784815828998E-4
5	0.0
6	5.640535444748699E-4
7	2.2562141778994797E-4
8	3.760356963165799E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.1281070889497398E-4
47	0.0
48	0.0
49	0.0
50	7.520713926331598E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2659322.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.08198063093458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.87512516546339	35.25269192779149
2	14.748565665585339	13.887833660023114
3	4.883456726510425	6.897684450287131
4	2.029797527505978	3.822675514990214
5	1.0363963977597188	2.4397797562646724
6	0.5895598469222728	1.665458717614272
7	0.3634450522627169	1.1978199037729569
8	0.24562071068861807	0.9251447634558321
9	0.16802816139000573	0.711998877601422
>10	0.8347298385830587	7.1098046411374805
>50	0.0926694545988059	3.0831756369461525
>100	0.105936706499418	10.578232079468911
>500	0.01831468114612206	6.02768206486133
>1k	0.00835406508419537	6.400018005785167
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	3527	0.13262779009085773	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	3340	0.1255959225697377	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	3199	0.12029381925167393	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	3116	0.1171727229722463	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2919	0.10976481975480969	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2808	0.10559082352569564	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2801	0.10532759853827404	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2779	0.10450032000637757	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2767	0.10404907717079766	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2739	0.10299617722111123	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2711	0.10194327727142483	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2706	0.10175525942326653	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.7603569631658E-5
2	0.0	0.0	0.0	0.0	3.7603569631658E-5
3	0.0	0.0	0.0	0.0	3.7603569631658E-5
4	0.0	0.0	0.0	0.0	3.7603569631658E-5
5	0.0	0.0	0.0	0.0	3.7603569631658E-5
6	0.0	0.0	0.0	0.0	3.7603569631658E-5
7	0.0	0.0	0.0	3.7603569631658E-5	3.7603569631658E-5
8	0.0	0.0	0.0	3.7603569631658E-5	3.7603569631658E-5
9	0.0	0.0	0.0	1.1281070889497398E-4	3.7603569631658E-5
10	0.0	0.0	0.0	1.1281070889497398E-4	3.7603569631658E-5
11	0.0	0.0	0.0	1.50414278526632E-4	3.7603569631658E-5
12	0.0	0.0	0.0	1.50414278526632E-4	7.5207139263316E-5
13	0.0	0.0	0.0	1.8801784815828998E-4	1.50414278526632E-4
14	0.0	0.0	0.0	2.2562141778994797E-4	1.50414278526632E-4
15	0.0	0.0	0.0	3.00828557053264E-4	1.50414278526632E-4
16	0.0	0.0	0.0	3.7603569631657996E-4	1.8801784815828998E-4
17	0.0	0.0	0.0	4.5124283557989594E-4	1.8801784815828998E-4
18	0.0	0.0	0.0	6.01657114106528E-4	1.8801784815828998E-4
19	0.0	0.0	0.0	6.01657114106528E-4	1.8801784815828998E-4
20	0.0	0.0	0.0	7.144678230015019E-4	3.3843212668492194E-4
21	0.0	0.0	0.0	9.400892407914499E-4	3.3843212668492194E-4
22	0.0	0.0	0.0	0.0013537285067396878	3.3843212668492194E-4
23	0.0	0.0	0.0	0.002369024886794454	3.3843212668492194E-4
24	0.0	0.0	0.0	0.0037603569631657996	3.3843212668492194E-4
25	0.0	0.0	0.0	0.0046628426343255916	3.3843212668492194E-4
26	0.0	0.0	0.0	0.005790949723275331	3.3843212668492194E-4
27	0.0	0.0	0.0	0.007407903217436625	3.3843212668492194E-4
28	0.0	0.0	0.0	0.011130656610970766	3.3843212668492194E-4
29	0.0	0.0	0.0	0.018087316992827496	3.3843212668492194E-4
30	0.0	0.0	0.0	0.03075971995869624	3.3843212668492194E-4
31	0.0	0.0	0.0	0.06351242910787035	3.3843212668492194E-4
32	0.0	0.0	0.0	0.10081517018247509	3.7603569631657996E-4
33	0.0	0.0	0.0	0.1366137684718135	3.7603569631657996E-4
34	0.0	0.0	0.0	0.18015870210527346	4.136392659482379E-4
35	0.0	0.0	0.0	0.23073550325985345	4.136392659482379E-4
36	0.0	0.0	0.0	0.3055666068268529	4.136392659482379E-4
37	0.0	0.0	0.0	0.4160834979742957	4.136392659482379E-4
38	0.0	0.0	0.0	0.5645423908800815	4.136392659482379E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATA	940	0.0	19.65944	36
GGCGTTA	810	0.0	18.46901	42
CAATACG	1040	0.0	17.76911	38
CGTCGTA	860	0.0	17.651043	10
ATACCGT	910	0.0	17.64823	6
TAGTACG	280	0.0	17.286572	2
ATACGAA	1060	0.0	17.2263	40
CCGTCGT	920	0.0	16.978144	9
TACCGTC	920	0.0	16.978144	7
AATACGA	1065	0.0	16.93885	39
CGAATGC	1055	0.0	16.890879	43
CTAGCGG	1115	0.0	16.771185	29
TAGCGGC	1130	0.0	16.743248	30
CGGTCCA	1135	0.0	16.669489	10
ACCGTCG	930	0.0	16.559027	8
CGCCGGT	1125	0.0	16.426554	7
TAGGACG	405	0.0	16.296185	4
CGGCGTT	920	0.0	16.260757	41
GCAATAC	1140	0.0	16.210417	37
TCTAGCG	1155	0.0	16.190365	28
>>END_MODULE