FastQCFastQC Report
Thu 2 Feb 2017
SRR4062363_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062363_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589688
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG34920.21966574573123784No Hit
GTCCTAAAGTGTGTATTTCTCATTT33940.21350101403545851No Hit
GCGCAAGACGGACCAGAGCGAAAGC32210.20261837542964403No Hit
GATTAAGAGGGACGGCCGGGGGCAT32200.2025554700041769No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG31920.20079411809109712No Hit
GAATAACGCCGCCGCATCGCCAGTC31640.19903276617801732No Hit
GTCCTACAGTGGACATTTCTAAATT31360.19727141426493755No Hit
GTCTTGCGCCGGTCCAAGAATTTCA30630.1926793182058366No Hit
TCCATGTACTCTGCGTTGATACCAC29880.18796141129580143No Hit
GAATAGGACCGCGGTTCTATTTTGT29480.18544519427711603No Hit
GTATCTGATCGTCTTCGAACCTCCG29040.18267735555656206No Hit
GAACTACGACGGTATCTGATCGTCT28500.1792804625813367No Hit
GTATTAGAGGCACTGCCTGCCCAGT28370.17846269205026394No Hit
CTGTAGGACGTGGAATATGGCAAGA27870.17531742077690715No Hit
GTCCTATTCCATTATTCCTAGCTGC27290.1716689060998133No Hit
CTATTGGAGCTGGAATTACCGCGGC26150.16449768759655983No Hit
CTTTAGGACGTGAAATATGGCGAGG25510.16047174036666315No Hit
GTATCAACGCAGAGTACTTTTTTTT25400.15977978068652465No Hit
CTCTTAATCATGGCCTCAGTTCCGA25250.15883619930451762No Hit
CCATAGGGTCTTCTCGTCTTATTAT25090.15782971249704345No Hit
GGGTAGGCACACGCTGAGCCAGTCA24320.152985994736074No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG24050.15128754824846133No Hit
ATCAGATACCGTCGTAGTTCCGACC23560.14820518240057168No Hit
CTCTAATACTTGTAATGCTAGAGGT23540.1480793715496374No Hit
TCGTAGTTCCGACCATAAACGATGC23150.14562605995641914No Hit
CTTTAATATACGCTATTGGAGCTGG22800.14342437006506936No Hit
GTGCATGGCCGTTCTTAGTTGGTGG21810.13719673294382295No Hit
GAATAATGGAATAGGACCGCGGTTC21280.13386274539406473No Hit
GTACATGGGGAATAATTGCAATCCC20890.13140943380084646No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC20830.13103200124804365No Hit
CCATTGGGATGTCCTGATCCAACAT20700.13021423071697089No Hit
GCTTTGAACACTCTAATTTTTTCAA20420.12845287880389109No Hit
CCATTATTCCTAGCTGCGGTATCCA19880.12505598582866576No Hit
GAATAATTGCAATCCCCGATCCCCA19640.12354625561745448No Hit
ATATTAAAGTTGCTGCAGTTAAAAA19070.11996064636582776No Hit
CATCTAAGGGCATCACAGACCTGTT18260.11486530690298977No Hit
CTTCTACACCATTGGGATGTCCTGA18080.11373300924458134No Hit
CTTTAAATCCTTTAACGAGGATCCA17980.11310395498990997No Hit
GATATATTTTGATCAACGGACCAAG17950.11291523871350857No Hit
ATACAGGACTCTTTCGAGGCCCTGT17660.11109098137496162No Hit
GTCCTACAGTGTGCATTTCTCATTT17660.11109098137496162No Hit
GTCAGGATACCGCGGCCGTTAAACT17640.11096517052402737No Hit
GCTAAGAGCATCGAGGGGGCGCCGA17580.11058773797122455No Hit
GTCCTGTATTGTTATTTTTCGTCAC17370.10926672403641469No Hit
GTTCTATTTTGTTGGTTTTCGGAAC17320.10895219690907902No Hit
TATCAACGCAGAGTACTTTTTTTTT17110.10763118297426916No Hit
GGATTGACAGATTGATAGCTCTTTC16970.10675050701772926No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT16960.10668760159226214No Hit
ATCTATAACTTTATAGATGCAACAC16730.105240776806518No Hit
CTGTTAGTATGAGTAACAAGAATTC16330.10272455978783258No Hit
ACCCCCGAGCGGTCGGCGTCCCCCA16110.10134064042755558No Hit
GTTATTGCTCAATCTCGGGTGGCTG16100.10127773500208845No Hit
CTCCAATAGCGTATATTAAAGTTGC16030.10083739702381851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGCGT1550.015.93659410
TGCTAAA901.3460522E-1015.83443511
TTCGCAG551.126922E-515.54751615
GCGTTAT3100.014.7005171
CGTTATT3300.014.38814452
CGCAGTT602.5635973E-514.25278817
GTCATAT551.9705806E-413.8095761
CGTTCAA1800.013.72404214
TAGGCCC903.6476195E-813.7197214
ATATATC1950.013.6363933
TTGCTAA1051.369699E-913.57237310
AATCGTA2350.013.342195513
TGCGAGG500.001498074513.30176419
CTCGACG1650.013.2441813
GTTATTC3600.013.1899633
TCGCAGT655.445855E-513.1555916
GACAATG655.4490745E-513.1547617
ATTGAAC1251.0186341E-1012.9208998
CGCTTAC1700.012.85505118
GTACGAA1700.012.85464619
Produced by FastQC (version 0.11.5)