##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062363_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1589688
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.201689891349748	32.0	32.0	32.0	32.0	32.0
2	30.92403855347716	32.0	32.0	32.0	32.0	32.0
3	30.958895078782756	32.0	32.0	32.0	32.0	32.0
4	30.86132121523217	32.0	32.0	32.0	32.0	32.0
5	30.87276308307039	32.0	32.0	32.0	32.0	32.0
6	34.32889975894641	36.0	36.0	36.0	32.0	36.0
7	34.2811344112807	36.0	36.0	36.0	32.0	36.0
8	34.24108693026556	36.0	36.0	36.0	32.0	36.0
9	34.36419599317602	36.0	36.0	36.0	32.0	36.0
10	34.11609573702513	36.0	36.0	36.0	32.0	36.0
11	34.390699307033834	36.0	36.0	36.0	32.0	36.0
12	34.1839008660819	36.0	36.0	36.0	32.0	36.0
13	34.33259859796388	36.0	36.0	36.0	32.0	36.0
14	34.19733117441913	36.0	36.0	36.0	32.0	36.0
15	34.14522220712492	36.0	36.0	36.0	32.0	36.0
16	34.180932359054104	36.0	36.0	36.0	32.0	36.0
17	34.074860601577164	36.0	36.0	36.0	32.0	36.0
18	34.129298956776424	36.0	36.0	36.0	32.0	36.0
19	34.085481553613036	36.0	36.0	36.0	32.0	36.0
20	34.034946480064015	36.0	36.0	36.0	32.0	36.0
21	34.00397184856399	36.0	36.0	36.0	32.0	36.0
22	33.95000780027276	36.0	36.0	36.0	32.0	36.0
23	33.94630455787551	36.0	36.0	36.0	32.0	36.0
24	33.916026289435415	36.0	36.0	36.0	32.0	36.0
25	33.30383509216903	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	10.0
4	56.0
5	155.0
6	238.0
7	70.0
8	234.0
9	291.0
10	190.0
11	46.0
12	127.0
13	92.0
14	350.0
15	566.0
16	928.0
17	1330.0
18	1658.0
19	2114.0
20	2654.0
21	3278.0
22	4328.0
23	5716.0
24	7431.0
25	10310.0
26	14502.0
27	19341.0
28	26769.0
29	37834.0
30	52393.0
31	77480.0
32	122167.0
33	191325.0
34	435538.0
35	570167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.24624424369008	19.13592440675407	11.441216437253077	26.176614912302774
2	16.719959890113	20.50158747333504	37.83307216193926	24.945380474612694
3	18.16463273090083	24.73447906437487	29.30798792912228	27.792900275602015
4	11.828334475228425	16.036107792380193	37.66687239652637	34.468685335865004
5	13.569686844183506	37.447998766632374	34.30007960255357	14.682234786630547
6	32.15535383438054	37.62467023119059	17.304492407765427	12.915483526663444
7	29.363469726383002	31.43706204788486	21.33083308784095	17.868635137891182
8	27.190186243035797	33.367730675734634	20.081269230454428	19.360813850775134
9	27.420008865516166	14.456898983487976	19.947852127220155	38.1752400237757
10	16.481162038025456	25.232786108849115	31.33595451908925	26.950097334036176
11	36.81746012252165	21.191509515361183	23.946640668446488	18.04438969367068
12	25.24536944304821	24.785557625758532	28.24176779377251	21.727305137420746
13	28.22713920785195	19.537607130440918	26.50322713920785	25.73202652249928
14	22.591279056218866	22.431996150505658	24.044223837515062	30.932500955760418
15	24.369219220448475	29.492043959587207	22.093086963832402	24.045649856131917
16	25.420095433439993	25.887753296307796	25.207678254413185	23.48447301583903
17	23.06383236014529	26.74806973699045	26.14425436958388	24.043843533280384
18	24.348672843957996	23.9746592905332	28.963123321804712	22.713544543704096
19	25.885900299446835	25.20946791182128	25.273212168854254	23.631419619877626
20	25.008518338202908	25.098204945233853	25.551109714306584	24.34216700225665
21	26.607425958037272	24.288144656841222	23.861979394663926	25.242449990457576
22	25.52006027785268	26.315491055341216	24.48573566952012	23.678712997285984
23	22.76751912133211	25.376095909653834	26.644510152479317	25.211874816534742
24	24.446373309767022	26.11627656485323	25.60304161624917	23.83430850913058
25	23.669530951790062	25.674824483275994	26.317808412457055	24.337836152476886
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	197.0
1	197.0
2	498.5
3	800.0
4	800.0
5	800.0
6	2809.5
7	4819.0
8	4819.0
9	4819.0
10	4812.0
11	4805.0
12	4805.0
13	4805.0
14	5676.5
15	6548.0
16	6548.0
17	6548.0
18	10544.0
19	14540.0
20	14540.0
21	14540.0
22	25046.0
23	35552.0
24	35552.0
25	35552.0
26	56024.0
27	76496.0
28	76496.0
29	76496.0
30	91114.0
31	105732.0
32	105732.0
33	105732.0
34	121188.5
35	136645.0
36	136645.0
37	136645.0
38	135817.0
39	134989.0
40	134989.0
41	134989.0
42	140725.5
43	146462.0
44	146462.0
45	146462.0
46	171302.5
47	196143.0
48	196143.0
49	196143.0
50	183490.5
51	170838.0
52	170838.0
53	170838.0
54	150921.5
55	131005.0
56	131005.0
57	131005.0
58	123305.5
59	115606.0
60	115606.0
61	115606.0
62	111157.5
63	106709.0
64	106709.0
65	106709.0
66	95431.0
67	84153.0
68	84153.0
69	84153.0
70	65017.5
71	45882.0
72	45882.0
73	45882.0
74	38992.5
75	32103.0
76	32103.0
77	32103.0
78	27414.0
79	22725.0
80	22725.0
81	22725.0
82	16257.5
83	9790.0
84	9790.0
85	9790.0
86	7372.0
87	4954.0
88	4954.0
89	4954.0
90	3220.0
91	1486.0
92	1486.0
93	1486.0
94	814.0
95	142.0
96	142.0
97	142.0
98	354.5
99	567.0
100	567.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008051894459793367
2	0.0034597984006924628
3	0.004843717760969448
4	0.01415372073010553
5	0.034157646028654676
6	0.04378217612512644
7	0.06567326418768966
8	0.0872498251229172
9	0.09511300330630916
10	0.11681537509247097
11	0.11870253785648505
12	0.13096909582257651
13	0.11977193008942635
14	0.12247686338451319
15	0.11165713020416584
16	0.11165713020416584
17	0.12272848508638173
18	0.1286415950802925
19	0.13172396092818214
20	0.12184780912984183
21	0.12838997337842392
22	0.14921166920804585
23	0.13801450347489572
24	0.13279335316112342
25	0.1314723392263136
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1589688.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.21085149206151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.26138466547857	21.295589156954374
2	12.198837353750559	6.6388150321743815
3	3.628770456348227	2.9622580195941564
4	1.5305707086494995	1.6659252900464352
5	0.806771536806176	1.0976470238027547
6	0.5233385366491764	0.8544292320500116
7	0.3742223746501792	0.7128036623128843
8	0.2634953452997804	0.5735946167841435
9	0.22415106146544608	0.5489407120791785
>10	1.4415528135020461	7.896076802611759
>50	0.268175174451902	5.281726685110287
>100	0.3764589975993837	22.295152737934394
>500	0.06686948388207455	12.699693238557275
>1k	0.03540149146698064	15.477347789987968
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3492	0.21966574573123784	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3394	0.21350101403545851	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3221	0.20261837542964403	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3220	0.2025554700041769	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3192	0.20079411809109712	No Hit
GAATAACGCCGCCGCATCGCCAGTC	3164	0.19903276617801732	No Hit
GTCCTACAGTGGACATTTCTAAATT	3136	0.19727141426493755	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	3063	0.1926793182058366	No Hit
TCCATGTACTCTGCGTTGATACCAC	2988	0.18796141129580143	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2948	0.18544519427711603	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2904	0.18267735555656206	No Hit
GAACTACGACGGTATCTGATCGTCT	2850	0.1792804625813367	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	2837	0.17846269205026394	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2787	0.17531742077690715	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2729	0.1716689060998133	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2615	0.16449768759655983	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2551	0.16047174036666315	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2540	0.15977978068652465	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	2525	0.15883619930451762	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	2509	0.15782971249704345	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2432	0.152985994736074	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	2405	0.15128754824846133	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2356	0.14820518240057168	No Hit
CTCTAATACTTGTAATGCTAGAGGT	2354	0.1480793715496374	No Hit
TCGTAGTTCCGACCATAAACGATGC	2315	0.14562605995641914	No Hit
CTTTAATATACGCTATTGGAGCTGG	2280	0.14342437006506936	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	2181	0.13719673294382295	No Hit
GAATAATGGAATAGGACCGCGGTTC	2128	0.13386274539406473	No Hit
GTACATGGGGAATAATTGCAATCCC	2089	0.13140943380084646	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	2083	0.13103200124804365	No Hit
CCATTGGGATGTCCTGATCCAACAT	2070	0.13021423071697089	No Hit
GCTTTGAACACTCTAATTTTTTCAA	2042	0.12845287880389109	No Hit
CCATTATTCCTAGCTGCGGTATCCA	1988	0.12505598582866576	No Hit
GAATAATTGCAATCCCCGATCCCCA	1964	0.12354625561745448	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	1907	0.11996064636582776	No Hit
CATCTAAGGGCATCACAGACCTGTT	1826	0.11486530690298977	No Hit
CTTCTACACCATTGGGATGTCCTGA	1808	0.11373300924458134	No Hit
CTTTAAATCCTTTAACGAGGATCCA	1798	0.11310395498990997	No Hit
GATATATTTTGATCAACGGACCAAG	1795	0.11291523871350857	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	1766	0.11109098137496162	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1766	0.11109098137496162	No Hit
GTCAGGATACCGCGGCCGTTAAACT	1764	0.11096517052402737	No Hit
GCTAAGAGCATCGAGGGGGCGCCGA	1758	0.11058773797122455	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	1737	0.10926672403641469	No Hit
GTTCTATTTTGTTGGTTTTCGGAAC	1732	0.10895219690907902	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1711	0.10763118297426916	No Hit
GGATTGACAGATTGATAGCTCTTTC	1697	0.10675050701772926	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	1696	0.10668760159226214	No Hit
ATCTATAACTTTATAGATGCAACAC	1673	0.105240776806518	No Hit
CTGTTAGTATGAGTAACAAGAATTC	1633	0.10272455978783258	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCA	1611	0.10134064042755558	No Hit
GTTATTGCTCAATCTCGGGTGGCTG	1610	0.10127773500208845	No Hit
CTCCAATAGCGTATATTAAAGTTGC	1603	0.10083739702381851	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.2581085093427139E-4	0.0
8	0.0	0.0	0.0	1.8871627640140707E-4	0.0
9	0.0	0.0	0.0	2.5162170186854277E-4	0.0
10	0.0	0.0	0.0	2.5162170186854277E-4	0.0
11	0.0	0.0	0.0	2.5162170186854277E-4	0.0
12	0.0	0.0	0.0	2.5162170186854277E-4	0.0
13	0.0	0.0	0.0	2.5162170186854277E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAGCGT	155	0.0	15.936594	10
TGCTAAA	90	1.3460522E-10	15.834435	11
TTCGCAG	55	1.126922E-5	15.547516	15
GCGTTAT	310	0.0	14.700517	1
CGTTATT	330	0.0	14.3881445	2
CGCAGTT	60	2.5635973E-5	14.252788	17
GTCATAT	55	1.9705806E-4	13.809576	1
CGTTCAA	180	0.0	13.724042	14
TAGGCCC	90	3.6476195E-8	13.719721	4
ATATATC	195	0.0	13.636393	3
TTGCTAA	105	1.369699E-9	13.572373	10
AATCGTA	235	0.0	13.3421955	13
TGCGAGG	50	0.0014980745	13.301764	19
CTCGACG	165	0.0	13.24418	13
GTTATTC	360	0.0	13.189963	3
TCGCAGT	65	5.445855E-5	13.15559	16
GACAATG	65	5.4490745E-5	13.154761	7
ATTGAAC	125	1.0186341E-10	12.920899	8
CGCTTAC	170	0.0	12.855051	18
GTACGAA	170	0.0	12.854646	19
>>END_MODULE