##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062363_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1589688
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19540375218282	32.0	32.0	32.0	32.0	32.0
2	31.21707781652752	32.0	32.0	32.0	32.0	32.0
3	31.372003185530744	32.0	32.0	32.0	32.0	32.0
4	31.524347544927053	32.0	32.0	32.0	32.0	32.0
5	31.39866124673521	32.0	32.0	32.0	32.0	32.0
6	34.99894885034045	36.0	36.0	36.0	36.0	36.0
7	35.00921375766817	36.0	36.0	36.0	36.0	36.0
8	34.9355911348642	36.0	36.0	36.0	36.0	36.0
9	35.12171948206189	36.0	36.0	36.0	36.0	36.0
10	34.942475504627325	36.0	36.0	36.0	32.0	36.0
11	35.13084768835142	36.0	36.0	36.0	36.0	36.0
12	34.992411089471645	36.0	36.0	36.0	36.0	36.0
13	35.07540221729044	36.0	36.0	36.0	36.0	36.0
14	35.02329136283346	36.0	36.0	36.0	36.0	36.0
15	34.965746737724636	36.0	36.0	36.0	32.0	36.0
16	34.98459320319459	36.0	36.0	36.0	36.0	36.0
17	34.951909431284626	36.0	36.0	36.0	32.0	36.0
18	34.98554747849892	36.0	36.0	36.0	36.0	36.0
19	34.95357579600525	36.0	36.0	36.0	32.0	36.0
20	34.94062356890157	36.0	36.0	36.0	32.0	36.0
21	34.93350519095571	36.0	36.0	36.0	32.0	36.0
22	34.910164761890385	36.0	36.0	36.0	32.0	36.0
23	34.86910135825395	36.0	36.0	36.0	32.0	36.0
24	34.83277913653497	36.0	36.0	36.0	32.0	36.0
25	34.80385018947114	36.0	36.0	36.0	32.0	36.0
26	34.762991857521726	36.0	36.0	36.0	32.0	36.0
27	34.74497008217965	36.0	36.0	36.0	32.0	36.0
28	34.703626120345625	36.0	36.0	36.0	32.0	36.0
29	34.67109835389083	36.0	36.0	36.0	32.0	36.0
30	34.64127489167686	36.0	36.0	36.0	32.0	36.0
31	34.65947657653577	36.0	36.0	36.0	32.0	36.0
32	34.61508044345809	36.0	36.0	36.0	32.0	36.0
33	34.606168631832155	36.0	36.0	36.0	32.0	36.0
34	34.590004453704125	36.0	36.0	36.0	32.0	36.0
35	34.5426071027774	36.0	36.0	36.0	32.0	36.0
36	34.49970245733754	36.0	36.0	36.0	32.0	36.0
37	34.4786297688603	36.0	36.0	36.0	32.0	36.0
38	34.43363225991515	36.0	36.0	36.0	32.0	36.0
39	34.37944049398372	36.0	36.0	36.0	32.0	36.0
40	34.38837620967133	36.0	36.0	36.0	32.0	36.0
41	34.33605462203904	36.0	36.0	36.0	32.0	36.0
42	34.30074832294136	36.0	36.0	36.0	32.0	36.0
43	34.32686036505277	36.0	36.0	36.0	32.0	36.0
44	34.25839913240837	36.0	36.0	36.0	32.0	36.0
45	34.19346563602418	36.0	36.0	36.0	32.0	36.0
46	34.18651269934729	36.0	36.0	36.0	32.0	36.0
47	34.172175294774824	36.0	36.0	36.0	32.0	36.0
48	34.13340101957114	36.0	36.0	36.0	32.0	36.0
49	34.12325060011776	36.0	36.0	36.0	32.0	36.0
50	33.55028785522694	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	9.0
21	29.0
22	111.0
23	357.0
24	954.0
25	2355.0
26	5020.0
27	9929.0
28	17141.0
29	27077.0
30	40209.0
31	59873.0
32	92676.0
33	156608.0
34	340007.0
35	837330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.446377105194784	20.01332527190035	12.018162131691183	26.52213549121369
2	16.103022910406025	21.05657490719521	37.371345604975254	25.46905657742351
3	17.70748541528536	25.046330137298245	29.69790108437055	27.54828336304585
4	11.569628757341063	16.626281383516766	38.08873187694692	33.71535798219525
5	13.49239599216953	37.62933355476043	33.97836556607334	14.899904886996692
6	32.46064307201063	37.89783113855476	16.8546287873927	12.786897002041917
7	29.338272667341016	31.562482701007998	21.252031845242588	17.847212786408402
8	27.281076538289273	33.55966705416409	19.853958764235497	19.30529764331114
9	27.494703363175667	14.13220707459577	19.72147993820171	38.65160962402685
10	16.55790318603399	25.311759288615125	31.050558348556446	27.07977917679444
11	36.91925711208741	21.029786977067197	24.04396334375047	18.006992567094926
12	25.132101393480983	24.747875054727718	28.225412785401915	21.89461076638938
13	28.44426076060208	19.36763691994907	26.449089381060936	25.739012938387912
14	22.734272385524708	22.355078480808814	23.99458258475877	30.91606654890771
15	24.518962211452813	29.455528380411756	21.93669449602689	24.08881491210854
16	25.61370533085738	25.945154017643713	24.911995309771477	23.529145341727435
17	23.248209711591205	26.894522698793725	25.856142840607717	24.001124749007353
18	24.57438189128936	23.998419815712264	28.749226263266753	22.67797202973162
19	26.037499182229467	25.25294271580335	25.145059911127216	23.564498190839963
20	25.167454242593518	25.095679152135514	25.416874254570708	24.31999235070026
21	26.86426518914403	24.288728354243098	23.723334390144483	25.123672066468387
22	25.69378393747704	26.04806729370795	24.504368152744437	23.753780616070575
23	22.819320520756275	25.22595628827795	26.672969790298474	25.281753400667302
24	24.72151768145699	26.109211367262002	25.330064767426062	23.839206183854944
25	24.074409569676565	25.45920960591009	26.11298569279003	24.353395131623312
26	22.549582056353195	26.783934960822503	27.3876383290306	23.2788446537937
27	24.97936702044678	25.847021553914985	25.07944955236499	24.094161873273244
28	22.61953288947265	25.887406837064887	26.68227979326761	24.810780480194854
29	23.647093014478312	25.357931870908	26.047375334027812	24.94759978058588
30	23.444474639048668	25.394794450231746	27.148220279702684	24.0125106310169
31	24.828771431878458	24.946467482927467	25.14115977474825	25.083601310445825
32	23.646841392776444	25.89074082461464	25.481541031950922	24.98087675065799
33	23.03156342628239	25.31050118010578	26.918804193024037	24.73913120058779
34	24.194999270297064	25.395738031613753	27.25176261002159	23.157500088067597
35	25.54274801093045	25.512490501280755	25.866962573788065	23.077798914000734
36	22.947962115836567	25.65440514113461	26.55332367105998	24.84430907196884
37	25.43687817986926	25.714542727881195	24.30118362848559	24.54739546376396
38	23.649420515220594	25.93490043329257	25.911499615018798	24.50417943646804
39	24.725229101559552	25.05976015419378	24.620177041029436	25.594833703217233
40	25.624147631484924	25.25539602739657	26.37171570773636	22.74874063338215
41	23.883365792532874	25.324214562857616	27.093052221568005	23.699367423041505
42	25.56363261218554	26.866844311588185	25.65509710081475	21.91442597541153
43	23.45982356286265	25.493367251938746	26.291259668563892	24.755549516634712
44	23.230596192460407	25.810662217994977	25.840731011368266	25.118010578176346
45	23.389369486339458	26.729395956942493	26.055741755614935	23.82549280110311
46	23.9324470792049	24.903896175788066	25.744564810557048	25.419091934449987
47	24.871562766758487	24.465130557147415	26.335247127264676	24.328059548829422
48	24.932754100175632	26.54483143862192	24.578722365646595	23.943692095555857
49	23.65527071978904	25.8180850582001	25.90413968023914	24.62250454177172
50	22.653879251777706	27.449725983966665	25.136001529859946	24.760393234395682
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18.0
1	21.5
2	25.0
3	56.0
4	87.0
5	101.5
6	116.0
7	124.0
8	132.0
9	183.5
10	235.0
11	352.5
12	470.0
13	933.0
14	1396.0
15	1916.5
16	2437.0
17	3828.5
18	5220.0
19	5852.0
20	6484.0
21	8095.5
22	9707.0
23	9539.5
24	9372.0
25	13565.5
26	17759.0
27	24442.0
28	31125.0
29	35148.0
30	39171.0
31	39485.5
32	39800.0
33	47895.0
34	55990.0
35	66119.0
36	76248.0
37	88639.5
38	101031.0
39	96475.0
40	91919.0
41	94057.0
42	96195.0
43	84692.5
44	73190.0
45	79115.5
46	85041.0
47	88987.5
48	92934.0
49	92091.0
50	91248.0
51	81163.0
52	71078.0
53	67827.0
54	64576.0
55	71849.5
56	79123.0
57	85374.0
58	91625.0
59	83301.0
60	74977.0
61	66472.0
62	57967.0
63	53581.0
64	49195.0
65	43577.0
66	37959.0
67	37989.0
68	38019.0
69	36967.5
70	35916.0
71	28022.5
72	20129.0
73	18238.0
74	16347.0
75	11929.0
76	7511.0
77	6842.0
78	6173.0
79	5474.0
80	4775.0
81	3745.5
82	2716.0
83	2355.5
84	1995.0
85	1631.5
86	1268.0
87	958.5
88	649.0
89	436.0
90	223.0
91	146.5
92	70.0
93	46.0
94	22.0
95	13.5
96	5.0
97	9.5
98	14.0
99	10.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014279531581039801
2	0.0038372309534952767
3	6.290542546713569E-4
4	0.0
5	0.0
6	3.7743255280281413E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	6.290542546713568E-5
47	6.290542546713568E-5
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1589688.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.35143913461118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.62858885156608	26.55728034457984
2	11.816451830266649	7.8819134800840605
3	3.3420888995745925	3.3439042354986506
4	1.4033022722494546	1.87208601281557
5	0.7848585642872173	1.3088081318051723
6	0.4575133403834675	0.915523699504312
7	0.3339912021193906	0.7797361074286332
8	0.2571791478810205	0.6861835757796018
9	0.18290124459650298	0.5490017754124411
>10	1.2324179028194917	8.103305449782928
>50	0.20892492137128937	4.972462874287091
>100	0.27854137946035784	19.78382591871769
>500	0.05028165157383957	11.415072123194902
>1k	0.022958791850696558	11.830896271109161
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	3161	0.19884404990161592	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	3156	0.19852952277428024	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2978	0.18733235704113008	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2967	0.1866403973609916	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2887	0.18160796332362072	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	2834	0.17827397577386256	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2825	0.1777078269446583	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2786	0.17525451535144004	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	2629	0.16537836355309973	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2602	0.16367991706548707	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2540	0.15977978068652465	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2531	0.15921363185732043	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2500	0.15726356366783922	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2441	0.15355214356527822	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	2239	0.14084524762091682	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC	2197	0.1382032197512971	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC	2099	0.13203848805551782	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	2097	0.13191267720458352	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	2091	0.1315352446517807	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	2091	0.1315352446517807	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2031	0.1277609191237526	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	2008	0.12631409433800847	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT	1972	0.12404949902119157	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	1964	0.12354625561745448	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	1901	0.11958321381302495	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1865	0.11731861849620807	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA	1826	0.11486530690298977	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC	1825	0.11480240147752263	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	1786	0.11234908988430434	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	1781	0.11203456275696866	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	1759	0.11065064339669169	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	1754	0.110336116269356	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT	1694	0.10656179074132786	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT	1685	0.10599564191212363	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	1678	0.10555530393385369	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	1658	0.10429719542451098	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	1634	0.10278746521329972	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1603	0.10083739702381851	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	6.290542546713569E-5	0.0	0.0	0.0	0.0
7	6.290542546713569E-5	0.0	0.0	0.0	0.0
8	6.290542546713569E-5	0.0	0.0	0.0	0.0
9	2.5162170186854277E-4	0.0	0.0	0.0	0.0
10	2.5162170186854277E-4	0.0	0.0	0.0	0.0
11	2.5162170186854277E-4	0.0	0.0	0.0	0.0
12	2.5162170186854277E-4	0.0	0.0	0.0	0.0
13	2.5162170186854277E-4	0.0	0.0	0.0	0.0
14	2.5162170186854277E-4	0.0	0.0	0.0	0.0
15	2.5162170186854277E-4	0.0	0.0	0.0	0.0
16	2.5162170186854277E-4	0.0	0.0	1.2581085093427139E-4	0.0
17	2.5162170186854277E-4	0.0	0.0	1.2581085093427139E-4	0.0
18	2.5162170186854277E-4	0.0	0.0	1.8871627640140707E-4	0.0
19	3.1452712733567845E-4	0.0	0.0	2.5162170186854277E-4	0.0
20	3.1452712733567845E-4	0.0	0.0	5.032434037370855E-4	0.0
21	3.1452712733567845E-4	0.0	0.0	5.661488292042212E-4	0.0
22	3.1452712733567845E-4	0.0	0.0	6.919596801384926E-4	0.0
23	3.1452712733567845E-4	0.0	0.0	0.001195203083875578	0.0
24	3.1452712733567845E-4	0.0	0.0	0.002075879040415478	0.0
25	3.1452712733567845E-4	0.0	0.0	0.002201689891349749	0.0
26	3.1452712733567845E-4	0.0	0.0	0.002642027869619699	0.0
27	3.1452712733567845E-4	0.0	0.0	0.004025947229896684	0.0
28	3.1452712733567845E-4	0.0	0.0	0.00962453009647176	0.0
29	3.1452712733567845E-4	0.0	0.0	0.020947506680556185	0.0
30	3.1452712733567845E-4	0.0	0.0	0.04208372963751378	0.0
31	3.1452712733567845E-4	0.0	0.0	0.08693529799558153	0.0
32	3.1452712733567845E-4	0.0	0.0	0.1376370709220929	0.0
33	3.1452712733567845E-4	0.0	0.0	0.1872065461901958	0.0
34	3.1452712733567845E-4	0.0	0.0	0.2523765669741484	0.0
35	3.1452712733567845E-4	0.0	0.0	0.3210063861587934	0.0
36	3.1452712733567845E-4	0.0	0.0	0.4261213521143772	0.0
37	3.7743255280281413E-4	0.0	0.0	0.5701118710086508	0.0
38	3.7743255280281413E-4	0.0	0.0	0.7617847024070132	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCTAG	30	0.005745652	29.333227	27
GCAATCG	45	4.070442E-5	29.333227	23
CCGAAGA	100	1.7462298E-9	24.199913	15
CGGTAGG	75	2.0633215E-6	23.468796	1
AACTCCG	95	2.6868292E-8	23.157812	23
TGTAGGA	2055	0.0	21.626587	2
TAGGACA	535	0.0	21.383102	4
CTGTAGG	2030	0.0	21.351692	1
GATATAC	560	0.0	20.823318	1
GTAACCA	245	0.0	20.652988	32
TTGGGTA	245	0.0	20.652988	28
CACATCG	75	5.4741777E-5	20.53326	36
AGTACCA	75	5.4741777E-5	20.53326	5
AGGACCT	1680	0.0	20.166594	5
TATAGGG	110	1.2654709E-7	20.001188	2
GTCGTAT	55	0.004484314	19.99993	43
AACGACG	55	0.004484314	19.99993	15
CTAGGGG	55	0.004484314	19.99993	3
ACGACGC	55	0.004484314	19.99993	16
GTACGAC	55	0.004484314	19.99993	3
>>END_MODULE