FastQCFastQC Report
Thu 2 Feb 2017
SRR4062362_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062362_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2108368
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG37910.1798073201642218No Hit
GTATCAACGCAGAGTACTTTTTTTT36720.1741631441949413No Hit
TCCATGTACTCTGCGTTGATACCAC32240.15291448172235586No Hit
TATCAACGCAGAGTACTTTTTTTTT24190.11473329134192893No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22530.10685990301503343No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG500.001499120613.30086613
TACTATC550.00308014712.0850932
ACGCTAT2650.011.83123910
ACGAACG1459.822543E-1111.7935915
TACGCTA2700.011.6121439
TTCCGTT907.4605414E-611.6115915
TAACCCG907.4664604E-611.6107635
CCTTATA1157.1251634E-811.5596542
AACCGCG1501.7644197E-1011.4010117
CGTTATT1251.8459104E-811.3945162
GCGTTAT1501.7826096E-1011.3928931
ATACGCT2900.011.1389198
AATACTA1451.2187229E-911.1375975
CGGTCCA3250.011.10867910
GCTCGTA1201.2743658E-711.0843179
CGAACGA1553.1286618E-1011.03323716
CCGATAA1553.1286618E-1011.0332379
GATATAC1553.1650416E-1011.025381
CTTATAC1408.38736E-910.8544973
GCCTTAT1408.436473E-910.8503741
Produced by FastQC (version 0.11.5)