##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062362_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2108368
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.164360775727957	32.0	32.0	32.0	32.0	32.0
2	30.819467948669303	32.0	32.0	32.0	32.0	32.0
3	30.810724218921933	32.0	32.0	32.0	32.0	32.0
4	30.883833846842677	32.0	32.0	32.0	32.0	32.0
5	30.646052302064916	32.0	32.0	32.0	32.0	32.0
6	34.29507657107298	36.0	36.0	36.0	32.0	36.0
7	34.18690997017598	36.0	36.0	36.0	32.0	36.0
8	34.17837161254582	36.0	36.0	36.0	32.0	36.0
9	34.35699887306201	36.0	36.0	36.0	32.0	36.0
10	34.0124712573896	36.0	36.0	36.0	32.0	36.0
11	34.345880320703024	36.0	36.0	36.0	32.0	36.0
12	34.119204522170705	36.0	36.0	36.0	32.0	36.0
13	34.23574062971929	36.0	36.0	36.0	32.0	36.0
14	34.13838333725422	36.0	36.0	36.0	32.0	36.0
15	34.07558927094321	36.0	36.0	36.0	32.0	36.0
16	34.0798295174277	36.0	36.0	36.0	32.0	36.0
17	33.999658029338335	36.0	36.0	36.0	32.0	36.0
18	34.00218794821397	36.0	36.0	36.0	32.0	36.0
19	33.99861599113627	36.0	36.0	36.0	32.0	36.0
20	33.99189373012681	36.0	36.0	36.0	32.0	36.0
21	33.969729667686096	36.0	36.0	36.0	32.0	36.0
22	33.91387841211781	36.0	36.0	36.0	32.0	36.0
23	33.87418372883671	36.0	36.0	36.0	32.0	36.0
24	33.842118643424676	36.0	36.0	36.0	32.0	36.0
25	33.36519573433101	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	85.0
5	195.0
6	312.0
7	102.0
8	341.0
9	364.0
10	245.0
11	80.0
12	172.0
13	106.0
14	327.0
15	460.0
16	677.0
17	941.0
18	1203.0
19	1681.0
20	2673.0
21	3860.0
22	6071.0
23	8727.0
24	12299.0
25	17372.0
26	24662.0
27	32263.0
28	44470.0
29	59611.0
30	78452.0
31	108083.0
32	156679.0
33	223702.0
34	483721.0
35	838421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.88062847688653	17.732073875063563	11.308893619604897	26.078404028445014
2	16.62177256977795	20.09657092579549	37.26641670164754	26.01523980277902
3	18.779413982865538	24.183902790255118	28.49749509786716	28.53918812901218
4	12.518459098716864	15.680165310034585	36.35995349232154	35.44142209892701
5	14.359370959668446	36.64000986878783	34.047982236181916	14.952636935361813
6	33.62579398559177	35.62294118931046	17.261268118245845	13.489996706851931
7	29.75241639040041	30.693390730268423	21.369552887684417	18.184639991646755
8	27.820963739774022	32.9399204281178	19.870563957763576	19.368551874344593
9	27.130969234815616	14.629865425462722	18.89289583552908	39.34626950419258
10	15.467006536866387	26.998122419751724	32.29732437691782	25.237546666464063
11	36.66033360859422	21.557770833204724	22.563715613560312	19.218179944640745
12	24.05658092331541	24.10516597279807	29.27593553528566	22.562317568600854
13	29.10198561528241	19.95483050517464	25.498920623825295	25.444263255717654
14	23.124411441086647	19.9785447570709	25.628710325252555	31.2683334765899
15	25.021106543170834	27.237032415281803	22.73357512077501	25.008285920772348
16	25.505937924226473	26.036887256123936	23.99352528609394	24.463649533555646
17	23.803714485162477	26.00766450914375	25.754222840617146	24.434398165076622
18	24.418586979957695	25.249590861901762	26.48522116253159	23.84660099560895
19	24.931243365952465	25.81882252928479	25.709400816388563	23.54053328837418
20	25.02094176195722	24.798275272575314	25.73077726702883	24.45000569843863
21	25.942642862129446	24.805036356961676	24.735794047345973	24.51652673356291
22	24.964398865703686	25.081723484398676	25.247022947175424	24.706854702722218
23	24.012257520008472	24.827984837683324	25.656535816247388	25.503221826060813
24	24.11846225889089	25.67579890025656	25.455764724432484	24.749974116420066
25	24.405998076626258	25.036009830576894	25.591461170408543	24.96653092238831
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	280.0
1	280.0
2	674.0
3	1068.0
4	1068.0
5	1068.0
6	2927.5
7	4787.0
8	4787.0
9	4787.0
10	5140.0
11	5493.0
12	5493.0
13	5493.0
14	6775.5
15	8058.0
16	8058.0
17	8058.0
18	12774.0
19	17490.0
20	17490.0
21	17490.0
22	27318.0
23	37146.0
24	37146.0
25	37146.0
26	53001.0
27	68856.0
28	68856.0
29	68856.0
30	83938.0
31	99020.0
32	99020.0
33	99020.0
34	120255.5
35	141491.0
36	141491.0
37	141491.0
38	158933.0
39	176375.0
40	176375.0
41	176375.0
42	198562.0
43	220749.0
44	220749.0
45	220749.0
46	250928.5
47	281108.0
48	281108.0
49	281108.0
50	278119.5
51	275131.0
52	275131.0
53	275131.0
54	255298.0
55	235465.0
56	235465.0
57	235465.0
58	213411.0
59	191357.0
60	191357.0
61	191357.0
62	167973.5
63	144590.0
64	144590.0
65	144590.0
66	119809.0
67	95028.0
68	95028.0
69	95028.0
70	72113.0
71	49198.0
72	49198.0
73	49198.0
74	38329.5
75	27461.0
76	27461.0
77	27461.0
78	21885.5
79	16310.0
80	16310.0
81	16310.0
82	11526.5
83	6743.0
84	6743.0
85	6743.0
86	4860.5
87	2978.0
88	2978.0
89	2978.0
90	2025.5
91	1073.0
92	1073.0
93	1073.0
94	649.5
95	226.0
96	226.0
97	226.0
98	556.5
99	887.0
100	887.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007588808025923368
2	0.0034623936618275366
3	0.0048378651165261475
4	0.015604486503304928
5	0.03381762576552101
6	0.045627708255864256
7	0.06654436037731554
8	0.08750844254892884
9	0.09609328162825465
10	0.11696250369954392
11	0.11795853475294635
12	0.13195039955074256
13	0.11961858650861709
14	0.12279639986947251
15	0.11316809968658223
16	0.11046458682734703
17	0.12132606831444985
18	0.1291045965410213
19	0.13057492809604396
20	0.1199031668095892
21	0.12896230639053524
22	0.14693829540194123
23	0.13683569471743073
24	0.13214011975139064
25	0.1277291250863227
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2108368.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.205785395577394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.76603889363452	45.395186493064784
2	12.102808298455809	13.604956918136407
3	3.2081207169595625	5.409448336216053
4	1.2850698216914396	2.889134344652878
5	0.6560300860309258	1.84363431142482
6	0.40368138940365383	1.3613537724606166
7	0.27884923499037423	1.0971058181712934
8	0.19308843250436905	0.8682149599767198
9	0.14665670745091453	0.7418659883227271
>10	0.7893572473991852	8.20178562319047
>50	0.07906016743428744	3.1342458417219103
>100	0.0788959561472561	9.345305425611958
>500	0.009130473787304923	3.3475847194496753
>1k	0.0032125741103480243	2.760177447599611
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3791	0.1798073201642218	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3672	0.1741631441949413	No Hit
TCCATGTACTCTGCGTTGATACCAC	3224	0.15291448172235586	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2419	0.11473329134192893	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2253	0.10685990301503343	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.743005016202105E-5	0.0
4	0.0	0.0	0.0	9.48601003240421E-5	0.0
5	0.0	0.0	0.0	9.48601003240421E-5	0.0
6	0.0	0.0	0.0	9.48601003240421E-5	0.0
7	0.0	0.0	0.0	1.4229015048606317E-4	0.0
8	0.0	0.0	0.0	1.897202006480842E-4	4.743005016202105E-5
9	0.0	0.0	0.0	1.897202006480842E-4	4.743005016202105E-5
10	0.0	0.0	0.0	3.794404012961684E-4	4.743005016202105E-5
11	0.0	0.0	0.0	4.2687045145818945E-4	4.743005016202105E-5
12	0.0	0.0	0.0	4.2687045145818945E-4	9.48601003240421E-5
13	0.0	0.0	0.0	4.2687045145818945E-4	9.48601003240421E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGACG	50	0.0014991206	13.300866	13
TACTATC	55	0.003080147	12.085093	2
ACGCTAT	265	0.0	11.831239	10
ACGAACG	145	9.822543E-11	11.79359	15
TACGCTA	270	0.0	11.612143	9
TTCCGTT	90	7.4605414E-6	11.61159	15
TAACCCG	90	7.4664604E-6	11.610763	5
CCTTATA	115	7.1251634E-8	11.559654	2
AACCGCG	150	1.7644197E-10	11.401011	7
CGTTATT	125	1.8459104E-8	11.394516	2
GCGTTAT	150	1.7826096E-10	11.392893	1
ATACGCT	290	0.0	11.138919	8
AATACTA	145	1.2187229E-9	11.137597	5
CGGTCCA	325	0.0	11.108679	10
GCTCGTA	120	1.2743658E-7	11.084317	9
CGAACGA	155	3.1286618E-10	11.033237	16
CCGATAA	155	3.1286618E-10	11.033237	9
GATATAC	155	3.1650416E-10	11.02538	1
CTTATAC	140	8.38736E-9	10.854497	3
GCCTTAT	140	8.436473E-9	10.850374	1
>>END_MODULE