##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062362_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2108368
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.180737423447898	32.0	32.0	32.0	32.0	32.0
2	31.24689997192141	32.0	32.0	32.0	32.0	32.0
3	31.39547697555645	32.0	32.0	32.0	32.0	32.0
4	31.540374355899917	32.0	32.0	32.0	32.0	32.0
5	31.430729834639873	32.0	32.0	32.0	32.0	32.0
6	35.02390616818316	36.0	36.0	36.0	36.0	36.0
7	35.04370347112079	36.0	36.0	36.0	36.0	36.0
8	34.97075842547411	36.0	36.0	36.0	36.0	36.0
9	35.146419410653166	36.0	36.0	36.0	36.0	36.0
10	34.96885932626562	36.0	36.0	36.0	36.0	36.0
11	35.151383914003624	36.0	36.0	36.0	36.0	36.0
12	35.04586675570868	36.0	36.0	36.0	36.0	36.0
13	35.10067881887792	36.0	36.0	36.0	36.0	36.0
14	35.061260652789265	36.0	36.0	36.0	36.0	36.0
15	35.002834894098186	36.0	36.0	36.0	36.0	36.0
16	35.026687940625166	36.0	36.0	36.0	36.0	36.0
17	35.0008504207994	36.0	36.0	36.0	36.0	36.0
18	35.01182858020991	36.0	36.0	36.0	36.0	36.0
19	34.995600862847475	36.0	36.0	36.0	36.0	36.0
20	34.99645982105591	36.0	36.0	36.0	36.0	36.0
21	34.99098165026219	36.0	36.0	36.0	36.0	36.0
22	34.970660245270274	36.0	36.0	36.0	36.0	36.0
23	34.923448373339	36.0	36.0	36.0	32.0	36.0
24	34.89814064717355	36.0	36.0	36.0	32.0	36.0
25	34.87730604903888	36.0	36.0	36.0	32.0	36.0
26	34.82286820896542	36.0	36.0	36.0	32.0	36.0
27	34.80438329551578	36.0	36.0	36.0	32.0	36.0
28	34.790578305115616	36.0	36.0	36.0	32.0	36.0
29	34.76802389336207	36.0	36.0	36.0	32.0	36.0
30	34.750060236163705	36.0	36.0	36.0	32.0	36.0
31	34.74648875338651	36.0	36.0	36.0	32.0	36.0
32	34.708377285179814	36.0	36.0	36.0	32.0	36.0
33	34.68762236952942	36.0	36.0	36.0	32.0	36.0
34	34.67744720086816	36.0	36.0	36.0	32.0	36.0
35	34.64353945800733	36.0	36.0	36.0	32.0	36.0
36	34.61352287646179	36.0	36.0	36.0	32.0	36.0
37	34.61114995105219	36.0	36.0	36.0	32.0	36.0
38	34.56997355300403	36.0	36.0	36.0	32.0	36.0
39	34.55420733002967	36.0	36.0	36.0	32.0	36.0
40	34.53528843162105	36.0	36.0	36.0	32.0	36.0
41	34.50751481714767	36.0	36.0	36.0	32.0	36.0
42	34.47613509596048	36.0	36.0	36.0	32.0	36.0
43	34.46391047483172	36.0	36.0	36.0	32.0	36.0
44	34.44290417991546	36.0	36.0	36.0	32.0	36.0
45	34.38531319010723	36.0	36.0	36.0	32.0	36.0
46	34.40499096931845	36.0	36.0	36.0	32.0	36.0
47	34.36583461710669	36.0	36.0	36.0	32.0	36.0
48	34.339686904752874	36.0	36.0	36.0	32.0	36.0
49	34.336076055034034	36.0	36.0	36.0	32.0	36.0
50	33.782068879816045	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	2.0
20	19.0
21	55.0
22	161.0
23	517.0
24	1356.0
25	3309.0
26	6996.0
27	13243.0
28	22091.0
29	34324.0
30	50005.0
31	73399.0
32	111256.0
33	184755.0
34	404129.0
35	1202749.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.21746419302489	18.379546108966675	11.95058535444553	26.4524043435629
2	16.050806835273367	20.727135951779136	36.79278887861731	26.429268334330185
3	18.20234258462166	24.49364551984841	28.944935743743343	28.35907615178658
4	12.272501332786938	16.31454530621847	36.59543608219454	34.817517278800054
5	14.2540106850417	36.836263878032675	33.849925629681344	15.059799807244275
6	33.80486909487426	35.9750877341098	16.932552757855753	13.287490413160189
7	29.75173214543192	30.69540042345549	21.24187997541226	18.310987455700335
8	27.782151882403834	33.23186464602005	19.64884688062046	19.33713659095566
9	27.191837478087315	14.43286940420268	18.672262147784448	39.70303096992555
10	15.327589870459047	27.19008256623132	32.266236254771464	25.216091308538168
11	37.0143162863409	21.36994111084972	22.447978720982295	19.16776388182708
12	24.028917152982782	24.069422415821144	29.22298194622571	22.678678484970366
13	29.30176325954482	19.802804823446383	25.464292761036024	25.431139155972772
14	23.206859523574632	19.766236254771464	25.544876416261296	31.482027805392608
15	25.032821594712118	27.27920363048576	22.596339917889097	25.091634856913025
16	25.591737305821376	26.03516084478611	23.91840513610527	24.454696713287245
17	23.838959802083888	26.017089995674382	25.657759935646908	24.486190266594825
18	24.622361940609988	25.144471932793515	26.39045935054981	23.842706776046686
19	25.14912007770939	25.717379508700567	25.552892094738677	23.58060831885136
20	25.1891984700963	24.71764891138549	25.598472372944382	24.49468024557383
21	26.096582759745928	24.757869593922884	24.66746791831407	24.47807972801712
22	25.04501111760376	25.097516183133116	25.148408626956964	24.709064072306163
23	24.17694633953845	24.703135316035908	25.56408558657692	25.555832757848723
24	24.289972149074547	25.630345366653263	25.304690642240825	24.774991842031373
25	24.661871172394953	24.835417725937788	25.53619671708165	24.966514384585615
26	24.199665333566056	25.368057189257282	25.837377535610486	24.59489994156618
27	25.09685216243085	25.3468085267847	25.048900381717043	24.507438929067412
28	24.275932854226586	24.942419919103305	25.894815326356692	24.886831900313418
29	24.270762978758924	25.408088151594026	25.792556138207374	24.528592731439673
30	24.67989459145652	25.17700894720466	25.84463433328527	24.298462128053547
31	24.599927526883352	25.170179019981333	25.178052408308226	25.05184104482709
32	24.254257321302543	25.300801378127534	25.34187580156785	25.10306549900207
33	24.145120775879732	24.944791421611406	25.60383196861269	25.30625583389617
34	24.48016665022425	25.171459631335708	26.065136636488507	24.28323708195154
35	25.41700500102449	24.964474892428644	25.382570784606862	24.235949321940005
36	24.364579617979405	25.32835823727167	25.43773193294529	24.869330211803632
37	25.28045388660803	25.172123652037975	25.112172068633182	24.435250392720814
38	24.321607992532613	25.068915862885415	25.650597998072445	24.958878146509527
39	24.632938841796122	25.128535435939074	25.211870034073748	25.026655688191056
40	24.995446715184446	25.09647272202955	25.58950809346376	24.318572469322245
41	24.045090800088033	25.408752172296296	25.946324360832644	24.59983266678303
42	25.182178822672324	25.456182222458317	25.384752566914315	23.976886387955044
43	24.395361720534556	24.720257564144397	25.72790897983654	25.15647173548451
44	24.34665105901816	25.015651916553466	25.680384069574192	24.95731295485418
45	24.389670114515113	25.29430346125534	25.664115562368618	24.651910861860927
46	24.26347025921009	25.05412957042109	25.70131291184125	24.98108725852757
47	24.435582403071948	24.857045828811668	26.02425193324884	24.683119834867536
48	24.737237522102404	25.785157050382097	25.171364771235382	24.306240656280117
49	24.01810310154584	25.34903773914231	25.812049888823964	24.820809270487885
50	23.852843316977847	25.912401315711463	25.317675070493014	24.917080296817677
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	46.0
2	46.0
3	185.0
4	324.0
5	350.5
6	377.0
7	339.5
8	302.0
9	407.0
10	512.0
11	763.5
12	1015.0
13	1788.0
14	2561.0
15	3336.5
16	4112.0
17	4776.5
18	5441.0
19	6124.5
20	6808.0
21	8422.5
22	10037.0
23	11043.5
24	12050.0
25	15174.5
26	18299.0
27	22692.5
28	27086.0
29	30632.5
30	34179.0
31	36591.5
32	39004.0
33	44027.0
34	49050.0
35	55769.0
36	62488.0
37	73833.5
38	85179.0
39	91243.0
40	97307.0
41	106610.5
42	115914.0
43	117893.0
44	119872.0
45	130819.5
46	141767.0
47	149329.5
48	156892.0
49	157737.0
50	158582.0
51	152585.5
52	146589.0
53	142812.5
54	139036.0
55	138799.5
56	138563.0
57	134652.0
58	130741.0
59	119169.0
60	107597.0
61	95042.0
62	82487.0
63	72195.5
64	61904.0
65	52769.0
66	43634.0
67	38768.5
68	33903.0
69	31126.5
70	28350.0
71	22083.0
72	15816.0
73	14031.0
74	12246.0
75	9082.0
76	5918.0
77	5281.5
78	4645.0
79	3963.0
80	3281.0
81	2586.0
82	1891.0
83	1569.5
84	1248.0
85	990.0
86	732.0
87	536.0
88	340.0
89	229.0
90	118.0
91	81.0
92	44.0
93	28.5
94	13.0
95	11.0
96	9.0
97	9.0
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015604486503304928
2	0.003272673461179452
3	6.165906521062736E-4
4	1.897202006480842E-4
5	0.0
6	4.2687045145818945E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	4.743005016202105E-5
47	0.0
48	0.0
49	0.0
50	1.4229015048606317E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2108368.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.06125675022588
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.3244417031666	48.03123731499498
2	11.893101922332791	14.048430923830038
3	3.151226435338471	5.583461807268734
4	1.1894158339975904	2.809935758180629
5	0.6225090463975393	1.8383083309311672
6	0.3526078811157268	1.2495278759237438
7	0.26594765589655633	1.0995041950919067
8	0.18003817038610703	0.8506624484811823
9	0.13515831007347331	0.7184357687858443
>10	0.7365118549099023	7.943534141906716
>50	0.0732198757673002	3.0312839657242296
>100	0.06638623536683273	8.359368986254578
>500	0.007419033530808846	2.8850174462032476
>1k	0.002016041720328483	1.5512910364229822
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.4229015048606317E-4	0.0
5	0.0	0.0	0.0	1.4229015048606317E-4	0.0
6	0.0	0.0	0.0	1.4229015048606317E-4	0.0
7	0.0	0.0	0.0	1.4229015048606317E-4	0.0
8	0.0	0.0	0.0	1.4229015048606317E-4	0.0
9	0.0	0.0	0.0	1.4229015048606317E-4	0.0
10	0.0	0.0	0.0	2.8458030097212633E-4	0.0
11	0.0	0.0	0.0	2.8458030097212633E-4	0.0
12	0.0	0.0	0.0	2.8458030097212633E-4	4.743005016202105E-5
13	0.0	0.0	0.0	2.8458030097212633E-4	4.743005016202105E-5
14	0.0	0.0	0.0	2.8458030097212633E-4	4.743005016202105E-5
15	0.0	0.0	0.0	2.8458030097212633E-4	4.743005016202105E-5
16	0.0	0.0	0.0	2.8458030097212633E-4	4.743005016202105E-5
17	0.0	0.0	0.0	3.794404012961684E-4	4.743005016202105E-5
18	0.0	0.0	0.0	6.165906521062737E-4	4.743005016202105E-5
19	0.0	0.0	0.0	7.114507524303157E-4	4.743005016202105E-5
20	0.0	0.0	0.0	9.011709530784E-4	4.743005016202105E-5
21	0.0	0.0	0.0	0.0011383212038885053	4.743005016202105E-5
22	0.0	0.0	0.0	0.001849771956318821	4.743005016202105E-5
23	0.0	0.0	0.0	0.0027035128592352	9.48601003240421E-5
24	0.0	0.0	0.0	0.004316134564743916	9.48601003240421E-5
25	0.0	0.0	0.0	0.005359595668308379	9.48601003240421E-5
26	0.0	0.0	0.0	0.006355626721710821	9.48601003240421E-5
27	0.0	0.0	0.0	0.008869419380297937	9.48601003240421E-5
28	0.0	0.0	0.0	0.012948403694231747	9.48601003240421E-5
29	0.0	0.0	0.0	0.0216281028738816	9.48601003240421E-5
30	0.0	0.0	0.0	0.03704286917653844	9.48601003240421E-5
31	0.0	0.0	0.0	0.0729948471993504	9.48601003240421E-5
32	0.0	0.0	0.0	0.11819568500375646	9.48601003240421E-5
33	0.0	0.0	0.0	0.16296965235670433	9.48601003240421E-5
34	0.0	0.0	0.0	0.21409924643136302	9.48601003240421E-5
35	0.0	0.0	0.0	0.26949754502060363	9.48601003240421E-5
36	0.0	0.0	0.0	0.353733314108353	9.48601003240421E-5
37	0.0	0.0	0.0	0.4744427917706966	9.48601003240421E-5
38	0.0	0.0	0.0	0.6361318327730263	9.48601003240421E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCGA	115	8.780262E-9	21.0434	10
ATTAGCG	55	0.0044847964	19.999926	44
TATGCCG	55	0.0044847964	19.999926	5
CGCGAAC	90	1.0025722E-5	19.555481	12
TATACCG	100	2.4845152E-5	17.599934	5
TAGGACG	220	1.8189894E-12	16.999937	4
GGCGTTA	495	0.0	16.444382	42
ACGCGAA	110	5.6246077E-5	15.999941	11
ATCGTTT	520	0.0	15.653787	29
CGTTTAT	535	0.0	15.626109	31
CCACGCG	170	4.4952685E-8	15.529353	9
ATTGTCG	285	0.0	15.438538	43
CGACCAT	585	0.0	15.418745	10
TCTATAC	300	0.0	15.3999405	3
GTCTAAA	305	0.0	15.15	1
CGTCGTA	320	0.0	15.124944	10
ACCGTCC	160	3.827481E-7	15.124944	8
TACCGTC	335	0.0	15.104421	7
TCGACGC	175	6.416485E-8	15.085657	14
GTATTAG	675	0.0	14.995026	1
>>END_MODULE