FastQCFastQC Report
Thu 2 Feb 2017
SRR4062361_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062361_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725979
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG233071.3503640542555848No Hit
TCCATGTACTCTGCGTTGATACCAC191921.1119486390042983No Hit
GAGTACATGGAAGCAGTGGTATCAA86650.5020339181415301No Hit
CATGTACTCTGCGTTGATACCACTG68730.3982087846955264No Hit
GCTTCCATGTACTCTGCGTTGATAC58330.33795312689204215No Hit
CCCATGTACTCTGCGTTGATACCAC51590.2989028255847841No Hit
GCGTTGATACCACTGCTTCCATGTA51020.2956003520320931No Hit
CATGGAAGCAGTGGTATCAACGCAG48930.28349128233889287No Hit
GTACATGGGAAGCAGTGGTATCAAC48320.2799570562561885No Hit
GTATCAACGCAGAGTACATGGAAGC38820.22491583037800578No Hit
GTATCAACGCAGAGTACTTTTTTTT37180.21541397664745632No Hit
ACTCTGCGTTGATACCACTGCTTCC30540.17694305666523172No Hit
ACGCAGAGTACATGGAAGCAGTGGT29740.17230800606496371No Hit
CAGTGGTATCAACGCAGAGTACATG29670.17190243913744024No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27900.16164738968434725No Hit
TATCAACGCAGAGTACTTTTTTTTT27340.15840285426415965No Hit
TATCAACGCAGAGTACATGGAAGCA23950.13876182734552392No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG20790.12045337747446522No Hit
GAATAGGACCGCGGTTCTATTTTGT20200.11703502765676756No Hit
GTATCAACGCAGAGTACATGGGAAG19510.11303729651403638No Hit
CTATTGGAGCTGGAATTACCGCGGC19130.11083564747890906No Hit
GTATCTGATCGTCTTCGAACCTCCG18940.1097348229613454No Hit
GATTAAGAGGGACGGCCGGGGGCAT18800.1089236891062985No Hit
GTCCTATTCCATTATTCCTAGCTGC18450.10689585446868125No Hit
GTCTTGCGCCGGTCCAAGAATTTCA17520.10150760814586968No Hit
GAATAACGCCGCCGCATCGCCAGTC17260.10000121670078257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGC954.8912625E-914.00218112
CGTCTTA1700.013.41307515
ACGCGCG852.696379E-713.41307513
CTTATGC655.468961E-513.1497913
AATCGTA604.089055E-412.66790513
ATGACCG604.0950638E-412.66575
AAATCGT701.0894482E-412.21618812
CGCATCG2800.012.2154813
GCGTTAT1800.012.1288141
CGGTCCA3400.012.01588110
TCTCGTC2000.011.8768512
GCCGTTA1603.6379788E-1211.87616115
CCGTCGT2650.011.8316889
ACGGTAT3400.011.7367819
GCATCGC3000.011.71781214
TAGACTG658.02934E-411.6914155
TTAGTAC658.0449856E-411.6887033
GTTTTCG2700.011.61224615
CGAACGA1157.053495E-811.5676916
TCGAACG1406.8394E-1011.5321583
Produced by FastQC (version 0.11.5)