##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062361_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1725979
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11475168585481	32.0	32.0	32.0	32.0	32.0
2	30.773165258673483	32.0	32.0	32.0	32.0	32.0
3	30.76499598199051	32.0	32.0	32.0	32.0	32.0
4	30.833615009220853	32.0	32.0	32.0	32.0	32.0
5	30.63374293661742	32.0	32.0	32.0	32.0	32.0
6	34.27471075835801	36.0	36.0	36.0	32.0	36.0
7	34.132374727618355	36.0	36.0	36.0	32.0	36.0
8	34.13785103990257	36.0	36.0	36.0	32.0	36.0
9	34.29519015005397	36.0	36.0	36.0	32.0	36.0
10	33.96153545321235	36.0	36.0	36.0	32.0	36.0
11	34.29814847109959	36.0	36.0	36.0	32.0	36.0
12	34.05661250803168	36.0	36.0	36.0	32.0	36.0
13	34.17355251715114	36.0	36.0	36.0	32.0	36.0
14	34.063561607644125	36.0	36.0	36.0	32.0	36.0
15	34.00901169712957	36.0	36.0	36.0	32.0	36.0
16	33.98834168897768	36.0	36.0	36.0	32.0	36.0
17	33.90923470100158	36.0	36.0	36.0	32.0	36.0
18	33.91446245869735	36.0	36.0	36.0	32.0	36.0
19	33.897786705400236	36.0	36.0	36.0	32.0	36.0
20	33.89894488866898	36.0	36.0	36.0	32.0	36.0
21	33.82585419637203	36.0	36.0	36.0	32.0	36.0
22	33.76801050302466	36.0	36.0	36.0	32.0	36.0
23	33.673669841869454	36.0	36.0	36.0	27.0	36.0
24	33.64246436370315	36.0	36.0	36.0	27.0	36.0
25	33.08911753851003	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	12.0
4	72.0
5	173.0
6	283.0
7	77.0
8	235.0
9	346.0
10	192.0
11	65.0
12	147.0
13	98.0
14	382.0
15	462.0
16	595.0
17	897.0
18	1267.0
19	1821.0
20	2935.0
21	3867.0
22	5838.0
23	8635.0
24	11723.0
25	16331.0
26	22145.0
27	28234.0
28	38091.0
29	50327.0
30	64801.0
31	88967.0
32	127597.0
33	180014.0
34	387236.0
35	682114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.84231941027385	16.969981770963102	11.949579851409592	25.23811896735345
2	15.387819948270586	20.52269419388332	37.564661765660205	26.524824092185895
3	19.02080576266551	25.448817638428896	27.812961362050864	27.71741523685473
4	12.2347631889725	16.28599507916107	35.35957703500332	36.119664696863104
5	13.85471117326822	37.31172955243984	33.83434460990265	14.99921466438929
6	33.911010175134734	34.41901337015255	17.997769515866697	13.672206938846015
7	29.51778205137868	30.594601927990535	21.776367244685037	18.111248775945747
8	28.50691065726921	32.791320998493426	20.25314914192334	18.448619202314028
9	27.152320185211178	15.500590963763889	18.314541188260524	39.03254766276441
10	15.754483091462374	27.487786570390078	31.830036393757954	24.927693944389596
11	36.76285643349553	21.39268071401556	21.62291662316026	20.221546229328656
12	24.07141605642922	23.952134022879406	29.25606534056297	22.720384580128403
13	30.46896254877836	20.083950694629348	24.83286142972581	24.614225326866485
14	24.19605968597686	20.027821898084603	24.440110405830765	31.336008010107776
15	25.76525063469995	26.919688198032183	23.908858421744704	23.40620274552317
16	26.06026542959608	25.286168277097314	25.227760077444515	23.42580621586209
17	23.913935080040492	25.668415694259533	26.7817602236899	23.635889002010078
18	25.631741935783296	23.538079718006227	28.272706915136165	22.557471431074312
19	24.639350139874015	27.465373784442015	24.893513581877063	23.001762493806904
20	24.634699130378287	23.74512211888137	27.76176390910362	23.85841484163673
21	25.68835495045283	25.595938066183948	23.38216789241352	25.333539090949703
22	24.360547028226453	26.12925537964474	24.581396212488713	24.928801379640095
23	22.836698295258728	25.560501431351412	25.057700809842036	26.545099463547828
24	23.469757166196345	27.07095648299421	24.862674977721667	24.596611373087775
25	25.139002661703724	24.80263478500999	24.35464707479457	25.703715478491716
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	312.0
1	312.0
2	735.0
3	1158.0
4	1158.0
5	1158.0
6	2866.5
7	4575.0
8	4575.0
9	4575.0
10	4443.0
11	4311.0
12	4311.0
13	4311.0
14	4528.0
15	4745.0
16	4745.0
17	4745.0
18	7714.0
19	10683.0
20	10683.0
21	10683.0
22	17948.0
23	25213.0
24	25213.0
25	25213.0
26	38860.5
27	52508.0
28	52508.0
29	52508.0
30	66066.0
31	79624.0
32	79624.0
33	79624.0
34	94903.5
35	110183.0
36	110183.0
37	110183.0
38	123158.5
39	136134.0
40	136134.0
41	136134.0
42	160718.0
43	185302.0
44	185302.0
45	185302.0
46	243796.5
47	302291.0
48	302291.0
49	302291.0
50	265791.0
51	229291.0
52	229291.0
53	229291.0
54	199631.5
55	169972.0
56	169972.0
57	169972.0
58	155624.0
59	141276.0
60	141276.0
61	141276.0
62	126265.5
63	111255.0
64	111255.0
65	111255.0
66	92543.0
67	73831.0
68	73831.0
69	73831.0
70	55693.5
71	37556.0
72	37556.0
73	37556.0
74	29529.5
75	21503.0
76	21503.0
77	21503.0
78	17851.0
79	14199.0
80	14199.0
81	14199.0
82	9902.5
83	5606.0
84	5606.0
85	5606.0
86	4084.0
87	2562.0
88	2562.0
89	2562.0
90	1777.0
91	992.0
92	992.0
93	992.0
94	594.5
95	197.0
96	197.0
97	197.0
98	448.5
99	700.0
100	700.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009328039333039393
2	0.00434535993775127
3	0.006431132707871881
4	0.014890100053361019
5	0.034878755767016864
6	0.04594493907515677
7	0.06825112008894663
8	0.08928266218766276
9	0.09716224820811842
10	0.1191787385593915
11	0.12138038759451882
12	0.13516966313031617
13	0.12224945958206906
14	0.12497255180972654
15	0.11216822452648613
16	0.11043008055138563
17	0.12537811873725
18	0.13053461253004817
19	0.13238863277015536
20	0.12317646970212268
21	0.1314036845175984
22	0.15243522661631456
23	0.1409634763806512
24	0.1350537868653095
25	0.13157749891510848
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1725979.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.297487826179484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.13081090472444	34.93831967980107
2	13.698919993603376	12.41053323284111
3	4.2460113456275925	5.7700094171515754
4	1.7251113150056427	3.1257283516109036
5	0.8625731465765089	1.9536198303119372
6	0.517695438100174	1.4070181683006773
7	0.310009863688983	0.9829867618513087
8	0.2278176736084968	0.8255654637495532
9	0.16493029779485008	0.672383534087734
>10	0.8778346947141814	7.5061919705589215
>50	0.099007644170704	3.196128510850211
>100	0.11321117710481825	11.021908136442063
>500	0.016355454293459012	5.131735722695512
>1k	0.008816612080067749	6.767186361982742
>5k	6.388849333382427E-4	1.8308527258611855
>10k+	2.5555397333529707E-4	2.459832131903595
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	23307	1.3503640542555848	No Hit
TCCATGTACTCTGCGTTGATACCAC	19192	1.1119486390042983	No Hit
GAGTACATGGAAGCAGTGGTATCAA	8665	0.5020339181415301	No Hit
CATGTACTCTGCGTTGATACCACTG	6873	0.3982087846955264	No Hit
GCTTCCATGTACTCTGCGTTGATAC	5833	0.33795312689204215	No Hit
CCCATGTACTCTGCGTTGATACCAC	5159	0.2989028255847841	No Hit
GCGTTGATACCACTGCTTCCATGTA	5102	0.2956003520320931	No Hit
CATGGAAGCAGTGGTATCAACGCAG	4893	0.28349128233889287	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4832	0.2799570562561885	No Hit
GTATCAACGCAGAGTACATGGAAGC	3882	0.22491583037800578	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3718	0.21541397664745632	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3054	0.17694305666523172	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2974	0.17230800606496371	No Hit
CAGTGGTATCAACGCAGAGTACATG	2967	0.17190243913744024	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2790	0.16164738968434725	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2734	0.15840285426415965	No Hit
TATCAACGCAGAGTACATGGAAGCA	2395	0.13876182734552392	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2079	0.12045337747446522	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2020	0.11703502765676756	No Hit
GTATCAACGCAGAGTACATGGGAAG	1951	0.11303729651403638	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1913	0.11083564747890906	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1894	0.1097348229613454	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1880	0.1089236891062985	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1845	0.10689585446868125	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1752	0.10150760814586968	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1726	0.10000121670078257	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.793813250335027E-5	0.0
4	0.0	0.0	0.0	5.793813250335027E-5	0.0
5	0.0	0.0	0.0	5.793813250335027E-5	5.793813250335027E-5
6	0.0	0.0	0.0	5.793813250335027E-5	5.793813250335027E-5
7	0.0	0.0	0.0	5.793813250335027E-5	5.793813250335027E-5
8	0.0	0.0	0.0	5.793813250335027E-5	5.793813250335027E-5
9	0.0	0.0	0.0	1.1587626500670054E-4	5.793813250335027E-5
10	0.0	0.0	0.0	1.1587626500670054E-4	5.793813250335027E-5
11	0.0	0.0	0.0	2.317525300134011E-4	5.793813250335027E-5
12	0.0	0.0	0.0	2.317525300134011E-4	1.1587626500670054E-4
13	0.0	0.0	0.0	2.317525300134011E-4	2.317525300134011E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCGC	95	4.8912625E-9	14.002181	12
CGTCTTA	170	0.0	13.413075	15
ACGCGCG	85	2.696379E-7	13.413075	13
CTTATGC	65	5.468961E-5	13.149791	3
AATCGTA	60	4.089055E-4	12.667905	13
ATGACCG	60	4.0950638E-4	12.6657	5
AAATCGT	70	1.0894482E-4	12.216188	12
CGCATCG	280	0.0	12.21548	13
GCGTTAT	180	0.0	12.128814	1
CGGTCCA	340	0.0	12.015881	10
TCTCGTC	200	0.0	11.87685	12
GCCGTTA	160	3.6379788E-12	11.876161	15
CCGTCGT	265	0.0	11.831688	9
ACGGTAT	340	0.0	11.736781	9
GCATCGC	300	0.0	11.717812	14
TAGACTG	65	8.02934E-4	11.691415	5
TTAGTAC	65	8.0449856E-4	11.688703	3
GTTTTCG	270	0.0	11.612246	15
CGAACGA	115	7.053495E-8	11.56769	16
TCGAACG	140	6.8394E-10	11.532158	3
>>END_MODULE