FastQCFastQC Report
Thu 2 Feb 2017
SRR4062361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725979
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA80540.46663371918198315No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC72690.42115228516685316No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA26380.15284079354383803No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC24600.1425278059582417No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19670.11396430663408999No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19610.11361667783906988No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19460.11274760585151962No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG19430.11257379145400957No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC19250.11153090506894929No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA19230.11141502880394258No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA17390.10075441242332613No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAC654.9337326E-420.30745118
GTCGTAT2200.019.99976343
TGGTATA8000.019.24977344
GACGCTT951.597918E-518.52609616
TCGTATA1557.221388E-1018.45139344
TTAGTAC851.430777E-418.1184833
GGCGTTA4200.017.80931342
TCCTATA1751.9099389E-1017.601832
TACTATA1751.9099389E-1017.601832
AGTCGTA2250.017.5997942
TTAGGAC6450.017.3961493
TAGGACG9750.017.1490124
CGATTAT902.2162625E-417.11090943
AGTACTC5550.017.0453385
ACTATAC1551.4393663E-817.0330433
TAGAACG1053.775298E-516.7621924
GCGAGAA7100.016.73219720
TAACGGC2500.016.71980336
AAAGTAC5400.016.7044733
TATGGCG12550.016.65318916
Produced by FastQC (version 0.11.5)