##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062361_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1725979
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18450514171957	32.0	32.0	32.0	32.0	32.0
2	31.37217370547382	32.0	32.0	32.0	32.0	32.0
3	31.476739867634542	32.0	32.0	32.0	32.0	32.0
4	31.598459193304205	32.0	32.0	32.0	32.0	32.0
5	31.527540601594804	32.0	32.0	32.0	32.0	32.0
6	35.11758080486495	36.0	36.0	36.0	36.0	36.0
7	35.12857572427011	36.0	36.0	36.0	36.0	36.0
8	35.07924603949411	36.0	36.0	36.0	36.0	36.0
9	35.200769534275906	36.0	36.0	36.0	36.0	36.0
10	35.08317714178446	36.0	36.0	36.0	36.0	36.0
11	35.20422438511709	36.0	36.0	36.0	36.0	36.0
12	35.10669191224227	36.0	36.0	36.0	36.0	36.0
13	35.15012581265473	36.0	36.0	36.0	36.0	36.0
14	35.10442421373609	36.0	36.0	36.0	36.0	36.0
15	35.059386585815936	36.0	36.0	36.0	36.0	36.0
16	35.056023277224114	36.0	36.0	36.0	36.0	36.0
17	35.039170812622864	36.0	36.0	36.0	36.0	36.0
18	35.04115693180508	36.0	36.0	36.0	36.0	36.0
19	35.00993175467372	36.0	36.0	36.0	36.0	36.0
20	35.002978599391994	36.0	36.0	36.0	36.0	36.0
21	34.95832741881564	36.0	36.0	36.0	36.0	36.0
22	34.92201643241314	36.0	36.0	36.0	32.0	36.0
23	34.84152530245154	36.0	36.0	36.0	32.0	36.0
24	34.80552196753263	36.0	36.0	36.0	32.0	36.0
25	34.7643638769649	36.0	36.0	36.0	32.0	36.0
26	34.700000405566925	36.0	36.0	36.0	32.0	36.0
27	34.64643544330493	36.0	36.0	36.0	32.0	36.0
28	34.58176084413542	36.0	36.0	36.0	32.0	36.0
29	34.55286304178672	36.0	36.0	36.0	32.0	36.0
30	34.5240063755121	36.0	36.0	36.0	32.0	36.0
31	34.50916436410872	36.0	36.0	36.0	32.0	36.0
32	34.45064858842431	36.0	36.0	36.0	32.0	36.0
33	34.41010058639184	36.0	36.0	36.0	32.0	36.0
34	34.3865110757431	36.0	36.0	36.0	32.0	36.0
35	34.29104873234263	36.0	36.0	36.0	32.0	36.0
36	34.2528321607621	36.0	36.0	36.0	32.0	36.0
37	34.17314231517301	36.0	36.0	36.0	32.0	36.0
38	34.09257644502048	36.0	36.0	36.0	32.0	36.0
39	33.925561087359696	36.0	36.0	36.0	32.0	36.0
40	33.82756684756883	36.0	36.0	36.0	32.0	36.0
41	33.83654494058155	36.0	36.0	36.0	32.0	36.0
42	33.643099365635386	36.0	36.0	36.0	27.0	36.0
43	33.72318898433874	36.0	36.0	36.0	27.0	36.0
44	33.6961208682145	36.0	36.0	36.0	27.0	36.0
45	33.4807984338164	36.0	36.0	36.0	21.0	36.0
46	33.65080629602098	36.0	36.0	36.0	27.0	36.0
47	33.52011061548257	36.0	36.0	36.0	21.0	36.0
48	33.55667479152412	36.0	36.0	36.0	21.0	36.0
49	33.610698044414214	36.0	36.0	36.0	21.0	36.0
50	33.01882699615696	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	16.0
21	57.0
22	227.0
23	733.0
24	2054.0
25	4844.0
26	10293.0
27	19552.0
28	30443.0
29	41640.0
30	54628.0
31	72836.0
32	104921.0
33	139840.0
34	291751.0
35	952140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.08144935346389	17.70468814736909	12.504063485992924	25.709799013174095
2	14.844274177568112	20.975098469336316	37.20165152689757	26.978975826198
3	18.49595038607343	25.584382551925966	28.27748155092189	27.642185511078715
4	12.073748418286232	16.803362271549545	35.45391129424743	35.66897801591679
5	13.644951647731517	37.38226247248663	33.74849867814151	15.224287201640344
6	34.129058872333964	34.68335523403624	17.509785790267745	13.677800103362047
7	29.267522529255878	30.7021294593558	21.88255006726621	18.147797944122114
8	28.421857057274195	32.9676855672552	20.194347784270715	18.416109591199888
9	27.21643774344879	15.012870956135618	18.344197698813254	39.42649360160234
10	15.760099051031329	27.328084524782746	31.919507711275745	24.99230871291018
11	36.8802864924776	21.18235505762237	21.693601138831934	20.243757311068094
12	23.980708919401682	23.921032642923233	29.323821436993153	22.77443700068193
13	30.734846715979742	19.73992731081896	24.907661101322788	24.61756487187851
14	24.185056712741	19.827124200236504	24.530657673123486	31.45716141389901
15	25.881774923101613	26.915565021358894	23.694320730437624	23.508339325101872
16	26.10854477371973	25.19034124980663	25.16108249289244	23.540031483581203
17	23.973119024043747	25.61954693539145	26.775412678833288	23.631921361731518
18	25.799155146151836	23.36668059113118	28.175719403306758	22.658444859410224
19	24.890337599704285	27.181269297019256	25.018264996271682	22.91012810700478
20	24.766176181749604	23.598491059277084	27.80265576811769	23.832676990855624
21	25.791912879588917	25.4699506772678	23.43927707115788	25.298859371985404
22	24.439347176298202	25.934208933017146	24.674460117996798	24.95198377268785
23	22.982376958236454	25.014325203261457	25.21032990552029	26.792967932981803
24	23.618421776858234	26.84256297440467	24.930256972999093	24.608758275738
25	25.315951121073894	24.298036071122535	24.468374180682385	25.917638627121185
26	23.16111609700929	25.15656331855718	26.13560188159879	25.54671870283474
27	25.82841390306603	25.660393318806314	24.953084597205414	23.558108180922247
28	24.18870681508871	24.014834479446158	25.677716820424813	26.118741885040315
29	23.445186760673216	25.660914761998843	26.789201954369084	24.104696522958854
30	25.233389282256617	24.358581419588536	26.61845827788171	23.789571020273133
31	23.878853682460797	24.882863580611353	25.316066997338904	25.92221573958895
32	23.607877036742625	25.695040322043315	25.37626471700988	25.320817924204174
33	23.165461456947043	24.996943763510448	26.012657164426685	25.82493761511583
34	23.50509478968168	25.93768522096735	27.001313457463848	23.555906531887118
35	25.770765461225192	24.921044809931058	25.92406975982906	23.384119969014687
36	24.010489119508406	26.023955100264835	25.76769474020252	24.19786104002424
37	25.6671141421767	25.646256414475495	25.398223269228655	23.288406174119153
38	24.20110557544443	25.598167764497713	24.81241081148728	25.38831584857058
39	24.38963625861033	25.05899550342154	25.82945678945109	24.721911448517044
40	25.69915392945105	25.157200638014714	25.073132407752354	24.070513024781878
41	23.249819377871923	25.321629058059226	26.650845693951087	24.77770587011777
42	26.312544938264022	25.862887091905524	25.514678915560385	22.30988905427007
43	24.708817430571287	24.030535713354563	25.167223934937795	26.09342292113635
44	24.196180834181643	24.99642232031792	26.630393533177404	24.177003312323038
45	24.790162568605993	25.345267816120593	25.56056591650304	24.30400369877038
46	23.767002688911845	24.66342251374149	26.922548794103278	24.64702600324338
47	25.069207139372573	24.091906154076124	27.10776151260329	23.73112519394801
48	24.423472127992287	27.594657872430663	24.210549491042475	23.771320508534576
49	23.33023750578657	25.229333612981385	27.433879554733863	24.006549326498178
50	23.20468557265181	27.920791620292018	23.910314088410114	24.964208718646056
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	34.0
2	36.0
3	143.0
4	250.0
5	270.5
6	291.0
7	289.5
8	288.0
9	375.0
10	462.0
11	710.0
12	958.0
13	1663.0
14	2368.0
15	3238.0
16	4108.0
17	4874.5
18	5641.0
19	6091.0
20	6541.0
21	7397.0
22	8253.0
23	8746.0
24	9239.0
25	10193.0
26	11147.0
27	14559.5
28	17972.0
29	20943.5
30	23915.0
31	26907.5
32	29900.0
33	35538.5
34	41177.0
35	47603.0
36	54029.0
37	62519.0
38	71009.0
39	74002.5
40	76996.0
41	84085.5
42	91175.0
43	95558.0
44	99941.0
45	116923.5
46	133906.0
47	146250.0
48	158594.0
49	152253.5
50	145913.0
51	129192.0
52	112471.0
53	106967.5
54	101464.0
55	103139.0
56	104814.0
57	102357.5
58	99901.0
59	91775.5
60	83650.0
61	73822.0
62	63994.0
63	55775.5
64	47557.0
65	40700.5
66	33844.0
67	29947.0
68	26050.0
69	24345.0
70	22640.0
71	17300.0
72	11960.0
73	10832.5
74	9705.0
75	6886.0
76	4067.0
77	3747.0
78	3427.0
79	2988.5
80	2550.0
81	1965.0
82	1380.0
83	1266.0
84	1152.0
85	951.0
86	750.0
87	524.0
88	298.0
89	197.5
90	97.0
91	64.5
92	32.0
93	24.0
94	16.0
95	13.0
96	10.0
97	7.0
98	4.0
99	4.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014136904330817466
2	0.0035342260827043665
3	6.952575900402032E-4
4	1.738143975100508E-4
5	0.0
6	4.055669275234519E-4
7	5.793813250335027E-5
8	5.793813250335027E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.1587626500670054E-4
47	5.793813250335027E-5
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1725979.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.98353592580841
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.17072122607203	39.85419773975797
2	13.124753487774571	13.382926819226682
3	4.004041987647324	6.124206555769879
4	1.6851396136924834	3.4365750413877447
5	0.8169243991790432	2.08248472271071
6	0.4764133017889518	1.4573540812374017
7	0.3022714380983325	1.0787606706542476
8	0.22258464037959136	0.9078521607460832
9	0.15470003403155283	0.7098439268494311
>10	0.8367964873330721	7.945676205981485
>50	0.09087587225322967	3.2756092974964686
>100	0.09633552910618323	10.458295220910621
>500	0.013172844745419228	4.604418882139939
>1k	0.005040044946073444	3.786937869086213
>5k	2.2909295209424743E-4	0.8948608060453087
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	8054	0.46663371918198315	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	7269	0.42115228516685316	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2638	0.15284079354383803	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2460	0.1425278059582417	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1967	0.11396430663408999	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1961	0.11361667783906988	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1946	0.11274760585151962	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1943	0.11257379145400957	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1925	0.11153090506894929	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1923	0.11141502880394258	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1739	0.10075441242332613	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.793813250335027E-5
2	0.0	0.0	0.0	0.0	5.793813250335027E-5
3	0.0	0.0	0.0	0.0	5.793813250335027E-5
4	0.0	0.0	0.0	0.0	5.793813250335027E-5
5	0.0	0.0	0.0	0.0	1.1587626500670054E-4
6	0.0	0.0	0.0	0.0	1.1587626500670054E-4
7	0.0	0.0	0.0	0.0	1.1587626500670054E-4
8	0.0	0.0	0.0	0.0	1.1587626500670054E-4
9	0.0	0.0	0.0	5.793813250335027E-5	1.1587626500670054E-4
10	0.0	0.0	0.0	5.793813250335027E-5	1.1587626500670054E-4
11	0.0	0.0	0.0	1.1587626500670054E-4	1.1587626500670054E-4
12	0.0	0.0	0.0	1.738143975100508E-4	9.270101200536043E-4
13	0.0	0.0	0.0	2.317525300134011E-4	9.270101200536043E-4
14	0.0	0.0	0.0	2.317525300134011E-4	9.270101200536043E-4
15	0.0	0.0	0.0	3.476287950201016E-4	0.0010428863850603049
16	0.0	0.0	0.0	4.635050600268022E-4	0.0011008245175636552
17	0.0	0.0	0.0	5.793813250335027E-4	0.0011008245175636552
18	0.0	0.0	0.0	7.531957225435535E-4	0.0011008245175636552
19	0.0	0.0	0.0	9.849482525569545E-4	0.0011008245175636552
20	0.0	0.0	0.0	0.0011008245175636552	0.0011587626500670055
21	0.0	0.0	0.0	0.0015643295775904574	0.0012167007825703558
22	0.0	0.0	0.0	0.0020857727701206097	0.0012167007825703558
23	0.0	0.0	0.0	0.004519174335261321	0.0012167007825703558
24	0.0	0.0	0.0	0.006836699635395332	0.0012167007825703558
25	0.0	0.0	0.0	0.008169276682972388	0.0012167007825703558
26	0.0	0.0	0.0	0.011239997705649953	0.0013325770475770562
27	0.0	0.0	0.0	0.01483216192085767	0.0013905151800804065
28	0.0	0.0	0.0	0.020741851436199398	0.0013905151800804065
29	0.0	0.0	0.0	0.030707210226775646	0.0013905151800804065
30	0.0	0.0	0.0	0.04594493907515677	0.0013905151800804065
31	0.0	0.0	0.0	0.07595689171189221	0.0014484533125837568
32	0.0	0.0	0.0	0.1153548218141704	0.0015643295775904574
33	0.0	0.0	0.0	0.1471628565585097	0.0015643295775904574
34	0.0	0.0	0.0	0.19322367189867315	0.001680205842597158
35	0.0	0.0	0.0	0.24055912615391034	0.001680205842597158
36	0.0	0.0	0.0	0.3118809672655345	0.001680205842597158
37	0.0	0.0	0.0	0.4245706349845508	0.001680205842597158
38	0.0	0.0	0.0	0.5550473093820956	0.001738143975100508
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTTAC	65	4.9337326E-4	20.307451	18
GTCGTAT	220	0.0	19.999763	43
TGGTATA	800	0.0	19.249773	44
GACGCTT	95	1.597918E-5	18.526096	16
TCGTATA	155	7.221388E-10	18.451393	44
TTAGTAC	85	1.430777E-4	18.118483	3
GGCGTTA	420	0.0	17.809313	42
TCCTATA	175	1.9099389E-10	17.60183	2
TACTATA	175	1.9099389E-10	17.60183	2
AGTCGTA	225	0.0	17.59979	42
TTAGGAC	645	0.0	17.396149	3
TAGGACG	975	0.0	17.149012	4
CGATTAT	90	2.2162625E-4	17.110909	43
AGTACTC	555	0.0	17.045338	5
ACTATAC	155	1.4393663E-8	17.033043	3
TAGAACG	105	3.775298E-5	16.762192	4
GCGAGAA	710	0.0	16.732197	20
TAACGGC	250	0.0	16.719803	36
AAAGTAC	540	0.0	16.704473	3
TATGGCG	1255	0.0	16.653189	16
>>END_MODULE