FastQCFastQC Report
Thu 2 Feb 2017
SRR4062360_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062360_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586466
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG106900.6738247148063684No Hit
TCCATGTACTCTGCGTTGATACCAC91890.5792119087329952No Hit
GTATCAACGCAGAGTACTTTTTTTT44010.2774090336635011No Hit
GAGTACATGGAAGCAGTGGTATCAA40850.25749054817436995No Hit
CATGTACTCTGCGTTGATACCACTG34180.21544741582864052No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31930.2012649498949237No Hit
CCCATGTACTCTGCGTTGATACCAC31010.1954658971575817No Hit
TATCAACGCAGAGTACTTTTTTTTT30730.19370096806360804No Hit
GCTTCCATGTACTCTGCGTTGATAC26730.16848769529255592No Hit
GTACATGGGAAGCAGTGGTATCAAC25610.1614279789166613No Hit
GCGTTGATACCACTGCTTCCATGTA24650.1553767934516088No Hit
CATGGAAGCAGTGGTATCAACGCAG23630.1489474088949905No Hit
GTATCAACGCAGAGTACATGGAAGC21080.13287394750344475No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20940.13199148295645793No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG16610.10469811518179399No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16490.10394171699866243No Hit
ACTCTGCGTTGATACCACTGCTTCC16200.10211375472276116No Hit
CTATTGGAGCTGGAATTACCGCGGC16140.10173555563119537No Hit
GTCTTGCGCCGGTCCAAGAATTTCA16110.10154645608541249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGCT1152.5465852E-1114.04567115
ACGCGCG1152.5465852E-1114.04522813
GGACCGT500.001499589313.2999776
CGCGCTA1251.0186341E-1012.92242416
AGGCTAT905.405291E-712.6666466
CGGTCCA3350.012.47837910
CGCGCGC1405.2750693E-1112.21580714
CCCTATA701.0951281E-412.2092582
TAAACTG802.8683437E-511.8742315
CGAACGA658.010955E-411.69450216
TCTAATC658.042823E-411.6889693
CGAGCCG3550.011.50883915
CGCCAGT2200.011.22973418
AGGCCCG3400.011.17715710
CACGCGC1451.2150849E-911.13861612
AAGACGG2900.011.137215
TCGGCGT1803.6379788E-1211.08471413
AGGATCG600.00587857711.0826165
GTTTAGA600.005897931311.0777231
ATCGCCA2150.011.04858516
Produced by FastQC (version 0.11.5)