FastQCFastQC Report
Thu 2 Feb 2017
SRR4062360_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062360_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586466
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA44340.27948912866711295No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC39760.25061993134425825No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA16800.10589574563841897No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC16500.10400475018059008No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16080.1013573565396296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG655.930451E-727.0783392
TATACGT551.5936996E-424.00055
ATACCGT3650.021.6990816
CGTCGTA3650.020.4929310
CCGTCGT3900.019.1792819
TGGTATA3700.019.02682344
TACCGTC4050.019.012147
ATACGTA708.123716E-418.8575346
ACCGTCG4100.018.7802878
TCGCTAC600.00741355518.33313627
GTCGTAG4100.018.24370611
CGCAATA4400.017.49981336
GTATAGG2150.017.399551
AATAGCG3700.017.2436035
ATAGCGT3750.017.0136876
AGCGTAT3750.017.013158
ATTGTCG1954.9112714E-1116.92289543
TAGCGTA3650.016.8765327
TTGTCGA2007.2759576E-1116.49982344
ATGCGTT1206.409724E-616.49982340
Produced by FastQC (version 0.11.5)