##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062360_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1586466
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22070879552414	32.0	32.0	32.0	32.0	32.0
2	31.376836314172508	32.0	32.0	32.0	32.0	32.0
3	31.481357936444905	32.0	32.0	32.0	32.0	32.0
4	31.60174122861757	32.0	32.0	32.0	32.0	32.0
5	31.518949665482904	32.0	32.0	32.0	32.0	32.0
6	35.11648847186136	36.0	36.0	36.0	36.0	36.0
7	35.14056210470316	36.0	36.0	36.0	36.0	36.0
8	35.0815435061325	36.0	36.0	36.0	36.0	36.0
9	35.198383072817194	36.0	36.0	36.0	36.0	36.0
10	35.07781761474875	36.0	36.0	36.0	36.0	36.0
11	35.21059890347477	36.0	36.0	36.0	36.0	36.0
12	35.116064258546984	36.0	36.0	36.0	36.0	36.0
13	35.15763779368735	36.0	36.0	36.0	36.0	36.0
14	35.115427623409516	36.0	36.0	36.0	36.0	36.0
15	35.07362338682329	36.0	36.0	36.0	36.0	36.0
16	35.081766643596524	36.0	36.0	36.0	36.0	36.0
17	35.05314075435591	36.0	36.0	36.0	36.0	36.0
18	35.068077097145476	36.0	36.0	36.0	36.0	36.0
19	35.03813948738895	36.0	36.0	36.0	36.0	36.0
20	35.02308212089008	36.0	36.0	36.0	36.0	36.0
21	34.995527165410415	36.0	36.0	36.0	36.0	36.0
22	34.97876349067676	36.0	36.0	36.0	36.0	36.0
23	34.91688822830114	36.0	36.0	36.0	32.0	36.0
24	34.88410025805785	36.0	36.0	36.0	32.0	36.0
25	34.86178399032819	36.0	36.0	36.0	32.0	36.0
26	34.80765865767057	36.0	36.0	36.0	32.0	36.0
27	34.771030705984245	36.0	36.0	36.0	32.0	36.0
28	34.7429727457128	36.0	36.0	36.0	32.0	36.0
29	34.71165218794478	36.0	36.0	36.0	32.0	36.0
30	34.69672908212341	36.0	36.0	36.0	32.0	36.0
31	34.68191439337496	36.0	36.0	36.0	32.0	36.0
32	34.63082347809534	36.0	36.0	36.0	32.0	36.0
33	34.595114550201515	36.0	36.0	36.0	32.0	36.0
34	34.56975945277113	36.0	36.0	36.0	32.0	36.0
35	34.50524310007274	36.0	36.0	36.0	32.0	36.0
36	34.473263215221756	36.0	36.0	36.0	32.0	36.0
37	34.43393114003074	36.0	36.0	36.0	32.0	36.0
38	34.37322577351169	36.0	36.0	36.0	32.0	36.0
39	34.30675665283719	36.0	36.0	36.0	32.0	36.0
40	34.24659400201454	36.0	36.0	36.0	32.0	36.0
41	34.22561971072812	36.0	36.0	36.0	32.0	36.0
42	34.11771824924077	36.0	36.0	36.0	32.0	36.0
43	34.13721567307462	36.0	36.0	36.0	32.0	36.0
44	34.104464892408664	36.0	36.0	36.0	32.0	36.0
45	33.980510770479796	36.0	36.0	36.0	32.0	36.0
46	34.03974053020991	36.0	36.0	36.0	32.0	36.0
47	33.96393367396465	36.0	36.0	36.0	32.0	36.0
48	33.94314406990128	36.0	36.0	36.0	32.0	36.0
49	33.951562151347716	36.0	36.0	36.0	32.0	36.0
50	33.38737924418172	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	15.0
21	38.0
22	143.0
23	488.0
24	1331.0
25	3437.0
26	7083.0
27	13032.0
28	21640.0
29	31120.0
30	42924.0
31	59406.0
32	86928.0
33	129847.0
34	278242.0
35	910789.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.53354616877296	17.26034893412062	12.057228524488053	26.148876372618368
2	15.77389394644246	20.398120027281795	36.68316937782636	27.14481664844939
3	18.914237412706647	24.700575683864905	27.520941264865634	28.864245638562814
4	12.495847681288502	16.139676752625178	34.776480762551664	36.587994803534656
5	14.674881150935477	36.465073944225715	32.76332426916177	16.096720635677034
6	34.8178964486971	34.175018594859	16.95800713538318	14.049077821060726
7	30.591326886299484	29.875522072329314	20.85446520757457	18.678685833796628
8	28.99967600944489	31.866172990785806	19.53700867210517	19.59714232766413
9	27.72186734540797	14.724362198748665	18.066192405005843	39.48757805083753
10	16.40829365394531	26.50948712421193	30.745821215204106	26.336398006638657
11	37.49680106601717	20.913716398586544	21.514296555993006	20.075185979403276
12	25.387811651809745	23.434161211144772	28.109773546990606	23.068253590054876
13	30.45807473970448	19.238168356586275	24.76227035435994	25.541486549349308
14	24.6378428532348	19.293259357591023	24.46210634202057	31.606791447153608
15	26.177554388181022	26.09996054122811	22.844611860575643	24.877873210015217
16	26.452694227295133	25.04491114212344	23.860706753249044	24.641687877332387
17	24.480890230235	25.157362338682326	25.540982283893886	24.820765147188784
18	25.86547710445733	23.589916203687945	26.739558238247778	23.805048453606947
19	25.71697092783583	25.42998085051933	24.897854728686276	23.955193492958564
20	25.783155768859846	23.259055031749813	26.02551835337158	24.93227084601876
21	26.820303744297075	24.171208207424552	23.784877835390105	25.22361021288827
22	25.55787517665049	24.467401129302488	24.508624830283157	25.466098863763865
23	24.242435703002773	24.0686532204283	25.053231522137885	26.63567955443104
24	24.68568503831787	25.45853488193255	24.67856228876005	25.17721779098953
25	25.576596031682996	23.830955091379206	24.43998169516397	26.15246718177383
26	24.435758471974818	24.64849546098057	25.563926362115545	25.351819704929067
27	26.08363494710886	24.69658977879135	24.573170808577053	24.64660446552274
28	24.918025346903118	23.704069296158885	25.4034438809278	25.9744614760102
29	24.421071740585678	24.747898788880445	25.89560696541874	24.93542250511514
30	25.27302822751953	24.25901342984974	25.853500799891076	24.614457542739647
31	25.010684124336734	24.351167941827935	24.63859925141793	25.9995486824174
32	24.484987387060297	24.720353288378067	24.761387889812955	26.033271434748677
33	24.184697308357066	24.26998120350515	25.366317336772426	26.17900415136536
34	24.759812060264768	24.673330534660057	25.822614540746542	24.74424286432864
35	26.161480926789483	24.344360358179753	25.154904044587152	24.339254670443612
36	24.869426763636916	24.97172961790546	25.023542893449967	25.13530072500766
37	25.99948564923547	24.73888504386479	24.844087424502007	24.41754188239773
38	24.949541937866933	24.695392148334726	24.692618688329908	25.662447225468433
39	25.116138637701656	24.49475753025908	24.945003548768145	25.44410028327112
40	25.973389911917433	24.602670337719182	24.622336690480605	24.801603059882783
41	24.36623287230864	24.920294541452513	25.574705036225172	25.13876755001368
42	26.451748729566216	25.20299836239793	24.580419624498727	23.764833283537122
43	25.304103586209852	23.708607685257675	24.78445803439847	26.202830694134004
44	24.813579364449033	24.589811568605946	25.358312122667613	25.238296944277405
45	25.296098372105043	24.721235752925054	24.836586475852616	25.146079399117284
46	24.62501512798273	24.43490681162636	25.63190844544849	25.30816961494241
47	25.382296730338666	24.07259162514876	25.821701595497913	24.723410049014664
48	25.5467183034493	26.07197380845225	23.885163627836967	24.496144260261488
49	24.48708291705143	24.88412918028447	25.608065731043546	25.02072217162055
50	24.39166599431188	26.1899419085463	23.852794642677047	25.565597454464772
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24.0
1	27.0
2	30.0
3	150.5
4	271.0
5	293.0
6	315.0
7	294.5
8	274.0
9	363.0
10	452.0
11	678.5
12	905.0
13	1682.5
14	2460.0
15	3408.0
16	4356.0
17	4998.5
18	5641.0
19	6224.0
20	6807.0
21	7160.5
22	7514.0
23	7857.0
24	8200.0
25	8361.5
26	8523.0
27	9952.0
28	11381.0
29	13783.0
30	16185.0
31	18654.0
32	21123.0
33	25043.5
34	28964.0
35	33673.5
36	38383.0
37	45588.5
38	52794.0
39	56952.0
40	61110.0
41	68373.5
42	75637.0
43	79622.5
44	83608.0
45	96715.5
46	109823.0
47	120179.0
48	130535.0
49	130181.5
50	129828.0
51	120759.5
52	111691.0
53	108973.5
54	106256.0
55	109187.0
56	112118.0
57	109789.5
58	107461.0
59	98819.0
60	90177.0
61	80416.5
62	70656.0
63	61507.0
64	52358.0
65	45298.0
66	38238.0
67	33355.0
68	28472.0
69	26141.0
70	23810.0
71	18643.5
72	13477.0
73	12131.0
74	10785.0
75	7834.0
76	4883.0
77	4462.5
78	4042.0
79	3483.0
80	2924.0
81	2299.0
82	1674.0
83	1404.5
84	1135.0
85	928.5
86	722.0
87	511.0
88	300.0
89	193.0
90	86.0
91	56.5
92	27.0
93	17.5
94	8.0
95	8.5
96	9.0
97	10.0
98	11.0
99	7.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01664076002889441
2	0.0037819909156578207
3	3.151659096381517E-4
4	1.8909954578289105E-4
5	0.0
6	3.781990915657821E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.2606636385526068E-4
47	1.2606636385526068E-4
48	0.0
49	6.303318192763034E-5
50	1.2606636385526068E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1586466.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.20304612819938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.72579886009974	37.012596810119376
2	14.940042748904094	15.00071310561006
3	5.1562715791462415	7.765816198122068
4	2.278965174974597	4.576439752152387
5	1.193023996730444	2.9946719369953625
6	0.6854949747106887	2.064836150162974
7	0.4167223661354309	1.4644512518824604
8	0.28006181791723705	1.1247965090917142
9	0.20652269733544967	0.9331261650766566
>10	0.9348029955053501	8.31590009318754
>50	0.08140532369401955	2.9172070202624667
>100	0.08658362495919014	9.421547325260866
>500	0.011012690886924702	3.884392778468825
>1k	0.0032911490006901412	2.523504903607314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	4434	0.27948912866711295	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3976	0.25061993134425825	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1680	0.10589574563841897	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1650	0.10400475018059008	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1608	0.1013573565396296	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.2606636385526068E-4	0.0	0.0	0.0
6	0.0	1.2606636385526068E-4	0.0	0.0	0.0
7	0.0	1.2606636385526068E-4	0.0	0.0	0.0
8	0.0	1.2606636385526068E-4	0.0	0.0	0.0
9	0.0	1.2606636385526068E-4	0.0	6.303318192763034E-5	0.0
10	0.0	1.2606636385526068E-4	0.0	6.303318192763034E-5	0.0
11	0.0	1.2606636385526068E-4	0.0	1.2606636385526068E-4	0.0
12	0.0	1.2606636385526068E-4	0.0	2.5213272771052136E-4	0.0
13	0.0	1.2606636385526068E-4	0.0	2.5213272771052136E-4	0.0
14	0.0	1.2606636385526068E-4	0.0	2.5213272771052136E-4	0.0
15	0.0	1.2606636385526068E-4	0.0	3.151659096381517E-4	0.0
16	0.0	1.2606636385526068E-4	0.0	6.303318192763034E-4	0.0
17	0.0	1.2606636385526068E-4	0.0	6.303318192763034E-4	0.0
18	0.0	1.2606636385526068E-4	0.0	8.194313650591945E-4	0.0
19	0.0	1.2606636385526068E-4	0.0	8.194313650591945E-4	0.0
20	0.0	1.8909954578289102E-4	0.0	0.0010085309108420855	0.0
21	0.0	1.8909954578289102E-4	0.0	0.0013236968204802372	0.0
22	0.0	1.8909954578289102E-4	0.0	0.0021431281855394317	0.0
23	0.0	1.8909954578289102E-4	0.0	0.004223223189151233	0.0
24	0.0	1.8909954578289102E-4	0.0	0.007059716375894598	0.0
25	0.0	1.8909954578289102E-4	0.0	0.00907677819757877	0.0
26	0.0	1.8909954578289102E-4	0.0	0.011724171838539243	0.0
27	0.0	1.8909954578289102E-4	0.0	0.015317063208414174	0.0
28	0.0	1.8909954578289102E-4	0.0	0.021620381401177207	0.0
29	0.0	2.5213272771052136E-4	0.0	0.03139052459995991	0.0
30	0.0	2.5213272771052136E-4	0.0	0.046896687354156974	0.0
31	0.0	2.5213272771052136E-4	0.0	0.07973697513845239	0.0
32	0.0	2.5213272771052136E-4	0.0	0.11780901702274112	0.0
33	0.0	2.5213272771052136E-4	0.0	0.15688958981787193	0.0
34	0.0	2.5213272771052136E-4	0.0	0.20473177490094335	0.0
35	0.0	3.151659096381517E-4	0.0	0.2569232495370213	0.0
36	0.0	3.151659096381517E-4	0.0	0.3275834464778949	0.0
37	0.0	3.151659096381517E-4	0.0	0.4286256371078863	0.0
38	0.0	3.151659096381517E-4	0.0	0.5623820491583179	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	65	5.930451E-7	27.078339	2
TATACGT	55	1.5936996E-4	24.0005	5
ATACCGT	365	0.0	21.699081	6
CGTCGTA	365	0.0	20.49293	10
CCGTCGT	390	0.0	19.179281	9
TGGTATA	370	0.0	19.026823	44
TACCGTC	405	0.0	19.01214	7
ATACGTA	70	8.123716E-4	18.857534	6
ACCGTCG	410	0.0	18.780287	8
TCGCTAC	60	0.007413555	18.333136	27
GTCGTAG	410	0.0	18.243706	11
CGCAATA	440	0.0	17.499813	36
GTATAGG	215	0.0	17.39955	1
AATAGCG	370	0.0	17.243603	5
ATAGCGT	375	0.0	17.013687	6
AGCGTAT	375	0.0	17.01315	8
ATTGTCG	195	4.9112714E-11	16.922895	43
TAGCGTA	365	0.0	16.876532	7
TTGTCGA	200	7.2759576E-11	16.499823	44
ATGCGTT	120	6.409724E-6	16.499823	40
>>END_MODULE