FastQCFastQC Report
Thu 2 Feb 2017
SRR4062359_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062359_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185267
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8540.46095634948479763No Hit
TATCAACGCAGAGTACTTTTTTTTT5840.3152207354790654No Hit
TCCATGTACTCTGCGTTGATACCAC2880.1554513216061144No Hit
GGTATCAACGCAGAGTACTTTTTTT2870.15491156007275986No Hit
GTACATGGAAGCAGTGGTATCAACG2850.15383203700605072No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2780.15005370627256878No Hit
GTCCTAAAGTGTGTATTTCTCATTT2550.1376391910054138No Hit
CTGTAGGACGTGGAATATGGCAAGA2340.12630419880496796No Hit
CTTTAGGACGTGAAATATGGCGAGG2300.12414515267154971No Hit
GTCCTACAGTGGACATTTCTAAATT2280.12306562960484058No Hit
GTACTTTTTTTTTTTTTTTTTTTTT2220.11982706040471319No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2110.11388968353781298No Hit
GAATAACGCCGCCGCATCGCCAGTC2060.11119087587104018No Hit
GAACTACGACGGTATCTGATCGTCT1960.10579326053749454No Hit
GAATAGGACCGCGGTTCTATTTTGT1890.1020149298040126No Hit
GTATCTGATCGTCTTCGAACCTCCG1890.1020149298040126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATGG250.00601937618.9962183
CCGGTGA350.002159470216.28687314
CCGGTCC350.002159470216.2868739
CGGTCCA350.002159470216.28687310
CCGCATC551.1168757E-515.5465612
TCGCCAG456.7189545E-414.77882917
CGGTTCT456.7189545E-414.77882912
GACCACG456.7189545E-414.77882911
TCGTCTT456.7189545E-414.7788299
GAACAGT456.7189545E-414.7788296
GATCGTC400.00525339914.2510147
ATAATTG400.00525339914.25101412
GGTCCAA400.00525339914.25101411
CGTCTTC400.00525339914.25101410
TGTTGGT400.00525339914.25101410
ACCCGGT400.00525339914.25101412
GATCAAA400.005272281414.2433141
TATCTGA400.005272281414.2433142
TAGGACC1257.2759576E-1213.6809734
TAACGCC500.001490058113.3009474
Produced by FastQC (version 0.11.5)