##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062359_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	185267
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.95376402705285	32.0	32.0	32.0	14.0	32.0
2	26.75989787711789	32.0	21.0	32.0	14.0	32.0
3	27.28170694187308	32.0	21.0	32.0	14.0	32.0
4	26.415659561605683	32.0	21.0	32.0	14.0	32.0
5	26.7194805335003	32.0	21.0	32.0	14.0	32.0
6	29.90996777623646	36.0	21.0	36.0	14.0	36.0
7	29.991174898929653	36.0	21.0	36.0	14.0	36.0
8	29.52211133121387	36.0	21.0	36.0	14.0	36.0
9	30.270506890055973	36.0	27.0	36.0	14.0	36.0
10	28.498782837742286	32.0	21.0	36.0	14.0	36.0
11	30.85901428748779	36.0	32.0	36.0	14.0	36.0
12	29.58321773440494	36.0	21.0	36.0	14.0	36.0
13	30.148067383829822	36.0	21.0	36.0	14.0	36.0
14	29.36992556688455	32.0	21.0	36.0	14.0	36.0
15	29.046403299022494	32.0	21.0	36.0	14.0	36.0
16	29.344826655583564	36.0	21.0	36.0	14.0	36.0
17	29.029859608025173	32.0	21.0	36.0	14.0	36.0
18	29.236259020764628	32.0	21.0	36.0	14.0	36.0
19	29.15841461242423	32.0	21.0	36.0	14.0	36.0
20	29.044605893116422	32.0	21.0	36.0	14.0	36.0
21	29.054990905018162	32.0	21.0	36.0	14.0	36.0
22	28.83666816000691	32.0	21.0	36.0	14.0	36.0
23	28.802323133639558	32.0	21.0	36.0	14.0	36.0
24	28.88876594320629	32.0	21.0	36.0	14.0	36.0
25	27.903290926068863	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	26.0
5	28.0
6	35.0
7	17.0
8	29.0
9	19.0
10	20.0
11	9.0
12	9.0
13	10.0
14	158.0
15	393.0
16	623.0
17	1038.0
18	1716.0
19	2757.0
20	3886.0
21	4952.0
22	6210.0
23	7418.0
24	8693.0
25	10033.0
26	11120.0
27	12410.0
28	13539.0
29	13978.0
30	14533.0
31	14974.0
32	15132.0
33	14529.0
34	16078.0
35	10889.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.863620415103505	18.988421365145342	11.830180022131657	26.317778197619496
2	15.748677534276153	20.654755478786573	38.38389290726546	25.212674079671814
3	18.550507149758978	24.731310492251055	29.38823542113133	27.32994693685864
4	12.275340801727628	15.62316102038062	37.11540018895938	34.98609798893238
5	14.004762753325018	36.66348069788916	34.18274500909891	15.14901153968691
6	32.808398950131235	36.867999524750765	16.951816208159165	13.371785316958837
7	28.515555363480683	31.189597705185477	21.708361919476214	18.586485011857622
8	27.13397338577657	34.05604871170086	19.97039262192783	18.839585280594743
9	26.996477122911667	14.074218159026561	19.464436231602154	39.464868486459615
10	15.527500499910825	26.88223181810812	32.22614344468284	25.364124237298213
11	36.7410465788663	21.117482071540902	23.594770888613876	18.54670046097892
12	24.40002594482401	23.895182911009254	29.887356222433624	21.817434921733113
13	29.259329316075338	20.24265679466047	25.83349096116951	24.664522928094684
14	22.78532322309717	20.85911785847471	25.594642944002764	30.760915974425355
15	24.265742957346895	29.11954001869755	22.73103090499376	23.8836861189618
16	24.21627171769029	26.44599962171364	25.585668350941663	23.752060309654407
17	22.380702731041342	26.553742548143184	27.04720004756217	24.018354673253306
18	23.633729886331086	24.670965510158855	28.996113744588158	22.699190858921902
19	24.856759853840998	25.170266588830515	26.298350305939334	23.67462325138916
20	24.678413144524917	24.82974813533672	26.744676251216088	23.747162468922276
21	25.480116968547133	24.549881896360677	25.604436588885825	24.365564546206357
22	24.75037706982955	25.7267041123587	26.067283313240964	23.45563550457079
23	22.830223356180674	25.481091483059092	27.100045406387164	24.58863975437307
24	23.90893071426255	25.41053610231241	26.869979784002336	23.81055339942271
25	23.608198387131647	25.243767971807234	26.86366289754178	24.28437074351934
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	25.0
2	78.5
3	132.0
4	132.0
5	132.0
6	281.5
7	431.0
8	431.0
9	431.0
10	496.0
11	561.0
12	561.0
13	561.0
14	620.5
15	680.0
16	680.0
17	680.0
18	1190.0
19	1700.0
20	1700.0
21	1700.0
22	2849.5
23	3999.0
24	3999.0
25	3999.0
26	5807.5
27	7616.0
28	7616.0
29	7616.0
30	9538.5
31	11461.0
32	11461.0
33	11461.0
34	13159.0
35	14857.0
36	14857.0
37	14857.0
38	15686.5
39	16516.0
40	16516.0
41	16516.0
42	18061.5
43	19607.0
44	19607.0
45	19607.0
46	21558.5
47	23510.0
48	23510.0
49	23510.0
50	22808.5
51	22107.0
52	22107.0
53	22107.0
54	19901.5
55	17696.0
56	17696.0
57	17696.0
58	16366.5
59	15037.0
60	15037.0
61	15037.0
62	13251.0
63	11465.0
64	11465.0
65	11465.0
66	9725.0
67	7985.0
68	7985.0
69	7985.0
70	6058.5
71	4132.0
72	4132.0
73	4132.0
74	3335.0
75	2538.0
76	2538.0
77	2538.0
78	2204.0
79	1870.0
80	1870.0
81	1870.0
82	1333.0
83	796.0
84	796.0
85	796.0
86	574.5
87	353.0
88	353.0
89	353.0
90	232.5
91	112.0
92	112.0
93	112.0
94	59.0
95	6.0
96	6.0
97	6.0
98	40.5
99	75.0
100	75.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006477138400254767
2	0.003778330733481948
3	0.008636184533673022
4	0.022669984400891685
5	0.04318092266836512
6	0.05451591486881096
7	0.08312327613660285
8	0.09607755293711238
9	0.1030944528707217
10	0.12630419880496796
11	0.12198610653813145
12	0.13979823713883205
13	0.12522467573825882
14	0.12954276800509534
15	0.11658849120458582
16	0.11982706040471319
17	0.13278133720522273
18	0.13817895253876836
19	0.14195728327225032
20	0.1333210987385773
21	0.14033799867218663
22	0.1554513216061144
23	0.14627537553908684
24	0.14303680633895946
25	0.1376391910054138
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	185267.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.60744919601696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.42221899208658	52.626450167660096
2	8.907938139791884	10.97590692776981
3	2.1084671965713437	3.896918570827121
4	0.8970261558160759	2.2105397328774914
5	0.4971648156462204	1.5314528060985833
6	0.34960106534168667	1.2922817923146823
7	0.2440078481481056	1.052289077574969
8	0.17428898879270968	0.8590000017977625
9	0.13943080298784602	0.7730978500290216
>10	1.078596083465702	13.491774869169026
>50	0.13046729883017968	5.5374626944204035
>100	0.04880074536396034	4.870512893995475
>500	0.001991867157712667	0.8823126154655445
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	854	0.46095634948479763	No Hit
TATCAACGCAGAGTACTTTTTTTTT	584	0.3152207354790654	No Hit
TCCATGTACTCTGCGTTGATACCAC	288	0.1554513216061144	No Hit
GGTATCAACGCAGAGTACTTTTTTT	287	0.15491156007275986	No Hit
GTACATGGAAGCAGTGGTATCAACG	285	0.15383203700605072	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	278	0.15005370627256878	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	255	0.1376391910054138	No Hit
CTGTAGGACGTGGAATATGGCAAGA	234	0.12630419880496796	No Hit
CTTTAGGACGTGAAATATGGCGAGG	230	0.12414515267154971	No Hit
GTCCTACAGTGGACATTTCTAAATT	228	0.12306562960484058	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	222	0.11982706040471319	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	211	0.11388968353781298	No Hit
GAATAACGCCGCCGCATCGCCAGTC	206	0.11119087587104018	No Hit
GAACTACGACGGTATCTGATCGTCT	196	0.10579326053749454	No Hit
GAATAGGACCGCGGTTCTATTTTGT	189	0.1020149298040126	No Hit
GTATCTGATCGTCTTCGAACCTCCG	189	0.1020149298040126	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.39761533354564E-4	0.0
10	0.0	0.0	0.0	5.39761533354564E-4	0.0
11	0.0	0.0	0.0	5.39761533354564E-4	0.0
12	0.0	0.0	0.0	5.39761533354564E-4	0.001079523066709128
13	0.0	0.0	0.0	5.39761533354564E-4	0.001079523066709128
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAATGG	25	0.006019376	18.996218	3
CCGGTGA	35	0.0021594702	16.286873	14
CCGGTCC	35	0.0021594702	16.286873	9
CGGTCCA	35	0.0021594702	16.286873	10
CCGCATC	55	1.1168757E-5	15.54656	12
TCGCCAG	45	6.7189545E-4	14.778829	17
CGGTTCT	45	6.7189545E-4	14.778829	12
GACCACG	45	6.7189545E-4	14.778829	11
TCGTCTT	45	6.7189545E-4	14.778829	9
GAACAGT	45	6.7189545E-4	14.778829	6
GATCGTC	40	0.005253399	14.251014	7
ATAATTG	40	0.005253399	14.251014	12
GGTCCAA	40	0.005253399	14.251014	11
CGTCTTC	40	0.005253399	14.251014	10
TGTTGGT	40	0.005253399	14.251014	10
ACCCGGT	40	0.005253399	14.251014	12
GATCAAA	40	0.0052722814	14.243314	1
TATCTGA	40	0.0052722814	14.243314	2
TAGGACC	125	7.2759576E-12	13.680973	4
TAACGCC	50	0.0014900581	13.300947	4
>>END_MODULE