FastQCFastQC Report
Thu 2 Feb 2017
SRR4062359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185267
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT2580.13925847560547752No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT2320.12522467573825882No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT2210.11928729887135862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTA300.00573262629.33333220
CTAATAT450.001393754924.4444454
CTAGCGG450.001393754924.44444529
ACACACT551.586528E-424.043
TAGAACA651.802663E-523.6923084
TAGGACG852.3303983E-723.2941194
GGCGTTA952.6542693E-823.15789442
CGGCGTT1004.5709385E-822.041
TCTTGCG500.002572274522.02
TGCGGCG1105.182301E-922.036
TCTAGCG500.002572274522.028
CCGTCGT602.860083E-422.09
ATGCGGC1105.182301E-922.035
ACCGTCG500.002572274522.08
CGGTCCA500.002572274522.010
CTGTAGG1551.8189894E-1221.2903231
TAGGACC1551.8189894E-1221.2903234
GTAGTTC1259.804353E-1021.123
CGACCAT1158.651114E-921.04347610
TTCCGAC1158.651114E-921.0434767
Produced by FastQC (version 0.11.5)