FastQCFastQC Report
Thu 2 Feb 2017
SRR4062358_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062358_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813590
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG63130.7759436571245959No Hit
GTCCTAAAGTGTGTATTTCTCATTT58960.7246893398394768No Hit
GTCCTACAGTGGACATTTCTAAATT52540.6457798153861282No Hit
TCCATGTACTCTGCGTTGATACCAC49950.6139455991346993No Hit
CTGTAGGACGTGGAATATGGCAAGA47910.5888715446355043No Hit
CTTTAGGACGTGAAATATGGCGAGG47580.584815447584164No Hit
GTCCTACAGTGTGCATTTCTCATTT31300.3847146597180398No Hit
CTGAAGGACCTGGAATATGGCGAGA26380.32424194004351087No Hit
CTGTAGGACCTGGAATATGGCGAGA24560.3018719502452095No Hit
GAGTACATGGAAGCAGTGGTATCAA23800.2925306358239408No Hit
GTCCTTCAGTGTGCATTTCTCATTT22650.278395752160179No Hit
ATTTAGAAATGTCCACTGTAGGACG22000.27040647008935703No Hit
GTATCAACGCAGAGTACTTTTTTTT21890.26905443773891025No Hit
TTGTAGAACAGTGTATATCAATGAG18730.23021423567152988No Hit
TTTCTAAATTTTCCACCTTTTTCAG18470.22701852284320112No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18110.22259368969628437No Hit
CATGTACTCTGCGTTGATACCACTG18110.22259368969628437No Hit
GCTTCCATGTACTCTGCGTTGATAC16620.20427979694932338No Hit
GTGTATATCAATGAGTTACAATGAA16130.19825710738824226No Hit
TATCAACGCAGAGTACTTTTTTTTT15800.19420101033690187No Hit
GATATACACTGTTCTACAAATCCCG15520.19075947344485553No Hit
GAATATGGCAAGAAAACTGAAAATC14790.1817868951191632No Hit
CATGGAAGCAGTGGTATCAACGCAG14240.17502673336692928No Hit
GGAATATGGCGAGAAAACTGAAAAT13820.16986442802885976No Hit
GCGTTGATACCACTGCTTCCATGTA13220.16248970611733182No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11960.1470027901031232No Hit
GTTCTACAGTGTGGTTTTTATCATT11740.14429872540222963No Hit
GCCATATTCCACGTCCTACAGTGGA11310.13901350803230128No Hit
ATTCCAGGTCCTTCAGTGTGCATTT11150.1370469155225605No Hit
GAAATATGGCGAGGAAAACTGAAAA11030.13557197114025493No Hit
CTGTAGGACATGGAATATGGCAAGA11020.1354490591083961No Hit
CCCATGTACTCTGCGTTGATACCAC10960.13471158691724333No Hit
GTGTATATCAATGAGTTACAATGAG10770.13237625831192615No Hit
GATATACACTGTTCTACAATGCCGG10700.13151587408891457No Hit
CCATATTCCAGGTCCTTCAGTGTGC10480.128811809388021No Hit
GTATCAACGCAGAGTACATGGAAGC10080.12389532811366905No Hit
CCATATTTCACGTCCTAAAGTGTGT10080.12389532811366905No Hit
ACAGTGGACATTTCTAAATTTTCCA10040.12340367998623385No Hit
GACCTGGAATATGGCGAGAAAACTG10030.12328076795437505No Hit
ACCTGGAATATGGCGAGAAAACTGA9980.12266620779508106No Hit
ATACACACTTTAGGACGTGAAATAT9550.11738099042515272No Hit
TTCCAGGTCCTTCAGTGTGCATTTC9430.11590604604284713No Hit
GTCCACTGTAGGACGTGGAATATGG9360.11504566181983554No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9220.11332489337381237No Hit
CCTAAAGTGTGTATTTCTCATTTTC8980.11037500460920119No Hit
CTGTAGAACATATTAGATGAGTGAG8960.1101291805454836No Hit
GAATAGGACCGCGGTTCTATTTTGT8840.10865423616317801No Hit
CCACTGTAGGACGTGGAATATGGCA8830.10853132413131922No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG8820.10840841209946042No Hit
GAAATACACACTTTAGGACGTGAAA8670.10656473162157844No Hit
GTGTATTTCTCATTTTCCGTGATTT8570.10533561130299045No Hit
GTTCTACAAATCCCGTTTCCAACGA8540.10496687520741405No Hit
CACTTTAGGACGTGAAATATGGCGA8360.10275445863395569No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGT402.7657722E-416.6197222
TAACCCC350.00216914116.2855575
CCAACGA1600.015.44020219
AACCGCG704.4258013E-714.9293467
AGAACCG704.4288936E-714.9284275
AACTTTT902.2791937E-914.77854315
TTGTAGA3850.014.548451
AACTCTT400.0052679614.2533718
GCTCGTA602.5625422E-514.2516169
CGGAATT400.00527441914.25073815
CCAGGAC602.569991E-514.2472313
CAGGACG602.569991E-514.2472314
CCTTACA400.005287358514.2454772
ACGCCAC551.9554344E-413.820600516
TCTTAAA551.9636203E-413.8137972
TAGAAAT6750.013.6491994
TCCAACG3000.013.61988718
CGCATCG1400.013.57213213
CGCCAGT1351.8189894E-1213.37353318
CGGTTCT1500.013.3006912
Produced by FastQC (version 0.11.5)