##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062358_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	813590
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16565837829865	32.0	32.0	32.0	32.0	32.0
2	30.95247237552084	32.0	32.0	32.0	32.0	32.0
3	30.928605317174497	32.0	32.0	32.0	32.0	32.0
4	30.961401934635383	32.0	32.0	32.0	32.0	32.0
5	30.873907004756695	32.0	32.0	32.0	32.0	32.0
6	34.48546688135301	36.0	36.0	36.0	32.0	36.0
7	34.34363500043019	36.0	36.0	36.0	32.0	36.0
8	34.34514681842206	36.0	36.0	36.0	32.0	36.0
9	34.433927408153984	36.0	36.0	36.0	32.0	36.0
10	34.20572892980494	36.0	36.0	36.0	32.0	36.0
11	34.47125333398886	36.0	36.0	36.0	32.0	36.0
12	34.26647082682924	36.0	36.0	36.0	32.0	36.0
13	34.36514091864453	36.0	36.0	36.0	32.0	36.0
14	34.29931783822318	36.0	36.0	36.0	32.0	36.0
15	34.250963015769614	36.0	36.0	36.0	32.0	36.0
16	34.292034071215234	36.0	36.0	36.0	32.0	36.0
17	34.216273553018105	36.0	36.0	36.0	32.0	36.0
18	34.24101451591096	36.0	36.0	36.0	32.0	36.0
19	34.15013458867489	36.0	36.0	36.0	32.0	36.0
20	34.106558586019986	36.0	36.0	36.0	32.0	36.0
21	34.03915485686894	36.0	36.0	36.0	32.0	36.0
22	33.997530697279956	36.0	36.0	36.0	32.0	36.0
23	33.974236409002074	36.0	36.0	36.0	32.0	36.0
24	33.91331997689254	36.0	36.0	36.0	32.0	36.0
25	33.43433055961848	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	29.0
5	79.0
6	170.0
7	25.0
8	92.0
9	140.0
10	86.0
11	30.0
12	61.0
13	45.0
14	136.0
15	246.0
16	305.0
17	413.0
18	586.0
19	760.0
20	1180.0
21	1722.0
22	2412.0
23	3452.0
24	4556.0
25	6253.0
26	8406.0
27	10750.0
28	14779.0
29	19998.0
30	26246.0
31	37127.0
32	54417.0
33	80156.0
34	184998.0
35	353929.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.75310378058914	18.75998741278684	12.145431480816791	25.341477325807226
2	12.88245300922853	20.55179432718262	44.71399914450492	21.851753519083932
3	19.573920450564618	26.404173278577897	28.976826454692173	25.045079816165313
4	10.964840033338168	16.949606752208407	38.362004322160786	33.72354889229264
5	11.408402385926427	39.83110139449555	35.574699146284935	13.185797073293093
6	31.00181504271991	37.44706125400268	18.926862140373142	12.624261562904266
7	27.328029833416966	31.2789650795525	22.603462526197717	18.789542560832817
8	24.52460678110384	40.10918851626141	20.18093112856837	15.185273574066374
9	27.077651018848982	14.881112755573437	19.98452318999445	38.05671303558313
10	15.904442907664404	28.16556863459281	33.94371119254404	21.98627726519874
11	35.593675013843594	19.694087245431614	26.87848397218975	17.83375376853504
12	26.00036428348147	22.984195511929055	32.79597417525481	18.219466029334665
13	31.46450414087592	21.73633757060409	25.805471124620063	20.993687163899928
14	20.615594385027066	24.36461144480829	25.709901269115804	29.30989290104884
15	21.782710610991824	36.18393124111752	22.767458886790248	19.265899261100415
16	21.02765329651805	25.9824586012654	32.73040927469466	20.259478827521885
17	19.96943190600188	29.47335259631903	30.15165909437714	20.405556403301944
18	21.010425217433042	24.606269898799948	36.32802452529017	18.055280358476843
19	24.963755362852506	25.636096458732805	25.984389576313117	23.415758602101576
20	24.144678065216375	26.975282358992786	29.606284871858612	19.273754703932227
21	22.877245578992323	25.989088550608525	24.790010596388367	26.343655274010793
22	23.528289438051768	30.909983899319045	25.96000925666061	19.60171740596858
23	19.603240929348676	29.960380709504076	28.549397341236677	21.88698101991057
24	23.967951163677988	27.228095654207333	29.11545704052873	19.68849614158595
25	22.398470988282583	28.194045666056656	29.085312798827395	20.32217054683337
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	199.0
1	199.0
2	415.5
3	632.0
4	632.0
5	632.0
6	1731.5
7	2831.0
8	2831.0
9	2831.0
10	2705.0
11	2579.0
12	2579.0
13	2579.0
14	2482.0
15	2385.0
16	2385.0
17	2385.0
18	4180.0
19	5975.0
20	5975.0
21	5975.0
22	12583.0
23	19191.0
24	19191.0
25	19191.0
26	34019.5
27	48848.0
28	48848.0
29	48848.0
30	66881.0
31	84914.0
32	84914.0
33	84914.0
34	87659.5
35	90405.0
36	90405.0
37	90405.0
38	88752.5
39	87100.0
40	87100.0
41	87100.0
42	91005.0
43	94910.0
44	94910.0
45	94910.0
46	108127.5
47	121345.0
48	121345.0
49	121345.0
50	102752.0
51	84159.0
52	84159.0
53	84159.0
54	66096.5
55	48034.0
56	48034.0
57	48034.0
58	43110.5
59	38187.0
60	38187.0
61	38187.0
62	34806.0
63	31425.0
64	31425.0
65	31425.0
66	26754.5
67	22084.0
68	22084.0
69	22084.0
70	16941.5
71	11799.0
72	11799.0
73	11799.0
74	9503.5
75	7208.0
76	7208.0
77	7208.0
78	6344.5
79	5481.0
80	5481.0
81	5481.0
82	3779.5
83	2078.0
84	2078.0
85	2078.0
86	1553.5
87	1029.0
88	1029.0
89	1029.0
90	691.0
91	353.0
92	353.0
93	353.0
94	216.0
95	79.0
96	79.0
97	79.0
98	219.5
99	360.0
100	360.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00811219410268071
2	0.0031957128283287652
3	0.004056097051340355
4	0.013274499440750254
5	0.03945476222667437
6	0.04744404429749628
7	0.06661832126746887
8	0.08407182979141829
9	0.09353605624454578
10	0.11357071743752997
11	0.11553730994727074
12	0.12733686500571542
13	0.11799555058444672
14	0.1200850551260463
15	0.1100062685136248
16	0.10865423616317801
17	0.12020796715790508
18	0.1279514251650094
19	0.12979510564289137
20	0.1194704949667523
21	0.1289347214198798
22	0.147740262294276
23	0.13606361926769012
24	0.13274499440750254
25	0.1277056011012918
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	813590.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.187613667401294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.82823265915323	27.802304022186604
2	13.060969879814092	9.452906642645543
3	3.977196328688755	4.317757326659863
4	1.6931676151098827	2.450867821190066
5	0.9237517520357255	1.6714185763626959
6	0.5561990187592697	1.2076509127828883
7	0.3910633497549888	0.9906154594289358
8	0.28018383912809564	0.8111347620973474
9	0.20815033231731417	0.6779217429576145
>10	1.5791129858914204	11.812200992382591
>50	0.23212392899751624	5.9544148424480605
>100	0.2328036430598985	16.571634971520133
>500	0.023790153305391054	5.978274228201996
>1k	0.012234935985629684	8.153176025225676
>5k	0.0010195779988024737	2.1477216739100053
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	6313	0.7759436571245959	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5896	0.7246893398394768	No Hit
GTCCTACAGTGGACATTTCTAAATT	5254	0.6457798153861282	No Hit
TCCATGTACTCTGCGTTGATACCAC	4995	0.6139455991346993	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4791	0.5888715446355043	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4758	0.584815447584164	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3130	0.3847146597180398	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2638	0.32424194004351087	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2456	0.3018719502452095	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2380	0.2925306358239408	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2265	0.278395752160179	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2200	0.27040647008935703	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2189	0.26905443773891025	No Hit
TTGTAGAACAGTGTATATCAATGAG	1873	0.23021423567152988	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1847	0.22701852284320112	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1811	0.22259368969628437	No Hit
CATGTACTCTGCGTTGATACCACTG	1811	0.22259368969628437	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1662	0.20427979694932338	No Hit
GTGTATATCAATGAGTTACAATGAA	1613	0.19825710738824226	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1580	0.19420101033690187	No Hit
GATATACACTGTTCTACAAATCCCG	1552	0.19075947344485553	No Hit
GAATATGGCAAGAAAACTGAAAATC	1479	0.1817868951191632	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1424	0.17502673336692928	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1382	0.16986442802885976	No Hit
GCGTTGATACCACTGCTTCCATGTA	1322	0.16248970611733182	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1196	0.1470027901031232	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1174	0.14429872540222963	No Hit
GCCATATTCCACGTCCTACAGTGGA	1131	0.13901350803230128	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	1115	0.1370469155225605	No Hit
GAAATATGGCGAGGAAAACTGAAAA	1103	0.13557197114025493	No Hit
CTGTAGGACATGGAATATGGCAAGA	1102	0.1354490591083961	No Hit
CCCATGTACTCTGCGTTGATACCAC	1096	0.13471158691724333	No Hit
GTGTATATCAATGAGTTACAATGAG	1077	0.13237625831192615	No Hit
GATATACACTGTTCTACAATGCCGG	1070	0.13151587408891457	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	1048	0.128811809388021	No Hit
GTATCAACGCAGAGTACATGGAAGC	1008	0.12389532811366905	No Hit
CCATATTTCACGTCCTAAAGTGTGT	1008	0.12389532811366905	No Hit
ACAGTGGACATTTCTAAATTTTCCA	1004	0.12340367998623385	No Hit
GACCTGGAATATGGCGAGAAAACTG	1003	0.12328076795437505	No Hit
ACCTGGAATATGGCGAGAAAACTGA	998	0.12266620779508106	No Hit
ATACACACTTTAGGACGTGAAATAT	955	0.11738099042515272	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	943	0.11590604604284713	No Hit
GTCCACTGTAGGACGTGGAATATGG	936	0.11504566181983554	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	922	0.11332489337381237	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	898	0.11037500460920119	No Hit
CTGTAGAACATATTAGATGAGTGAG	896	0.1101291805454836	No Hit
GAATAGGACCGCGGTTCTATTTTGT	884	0.10865423616317801	No Hit
CCACTGTAGGACGTGGAATATGGCA	883	0.10853132413131922	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	882	0.10840841209946042	No Hit
GAAATACACACTTTAGGACGTGAAA	867	0.10656473162157844	No Hit
GTGTATTTCTCATTTTCCGTGATTT	857	0.10533561130299045	No Hit
GTTCTACAAATCCCGTTTCCAACGA	854	0.10496687520741405	No Hit
CACTTTAGGACGTGAAATATGGCGA	836	0.10275445863395569	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	1.2291203185879864E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCCGT	40	2.7657722E-4	16.619722	2
TAACCCC	35	0.002169141	16.285557	5
CCAACGA	160	0.0	15.440202	19
AACCGCG	70	4.4258013E-7	14.929346	7
AGAACCG	70	4.4288936E-7	14.928427	5
AACTTTT	90	2.2791937E-9	14.778543	15
TTGTAGA	385	0.0	14.54845	1
AACTCTT	40	0.00526796	14.25337	18
GCTCGTA	60	2.5625422E-5	14.251616	9
CGGAATT	40	0.005274419	14.250738	15
CCAGGAC	60	2.569991E-5	14.247231	3
CAGGACG	60	2.569991E-5	14.247231	4
CCTTACA	40	0.0052873585	14.245477	2
ACGCCAC	55	1.9554344E-4	13.8206005	16
TCTTAAA	55	1.9636203E-4	13.813797	2
TAGAAAT	675	0.0	13.649199	4
TCCAACG	300	0.0	13.619887	18
CGCATCG	140	0.0	13.572132	13
CGCCAGT	135	1.8189894E-12	13.373533	18
CGGTTCT	150	0.0	13.30069	12
>>END_MODULE