FastQCFastQC Report
Thu 2 Feb 2017
SRR4062358_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062358_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813590
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA25740.31637557000454775No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA22920.28171437702036656No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA21050.25872982706277114No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20060.2465615359087501No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC20050.24643862387689128No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA19780.24311999901670375No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA14200.1745350852394941No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA11720.14405290133851203No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG9790.12033087918976387No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT8990.11049791664106No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA8910.10951462038618962No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC8350.10263154660209689No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC8330.10238572253837927No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA8280.10177116237908529No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8230.1011566022197913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCAC407.0303725E-427.4998467
AATCGCG407.0303725E-427.49984619
ACGCACC450.001397866524.4443078
CGAGCAA450.001397866524.44430721
CGCACCG450.001397866524.4443079
TATAAGG450.001397866524.4443072
AGCGCAA1550.024.12889942
GTCCTAG1202.3646862E-1123.8390621
CTAGGAC2150.023.5347523
CGCAATC1550.022.7095544
GATATAC9900.022.6721131
ATACCGT2150.022.5115036
ACTGTTC9600.022.4582088
TGTAGGA33250.022.2314572
CTGTAGG33000.022.0052871
CAGCGCA1600.021.99987641
ACCGGAT500.002579803321.99987612
CGACGTG500.002579803321.9998766
GTCGTAG2100.021.99987611
GACGGTT703.213827E-521.99987621
Produced by FastQC (version 0.11.5)