##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062358_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	813590
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.203184650745463	32.0	32.0	32.0	32.0	32.0
2	31.40500743617793	32.0	32.0	32.0	32.0	32.0
3	31.505462210695804	32.0	32.0	32.0	32.0	32.0
4	31.612905763345175	32.0	32.0	32.0	32.0	32.0
5	31.552673951253087	32.0	32.0	32.0	32.0	32.0
6	35.15615113263437	36.0	36.0	36.0	36.0	36.0
7	35.1721149473322	36.0	36.0	36.0	36.0	36.0
8	35.100346611929844	36.0	36.0	36.0	36.0	36.0
9	35.23837805282759	36.0	36.0	36.0	36.0	36.0
10	35.12501014024263	36.0	36.0	36.0	36.0	36.0
11	35.24601089000602	36.0	36.0	36.0	36.0	36.0
12	35.15820990916801	36.0	36.0	36.0	36.0	36.0
13	35.190266596197105	36.0	36.0	36.0	36.0	36.0
14	35.15045415995772	36.0	36.0	36.0	36.0	36.0
15	35.092520802861394	36.0	36.0	36.0	36.0	36.0
16	35.123286913555965	36.0	36.0	36.0	36.0	36.0
17	35.12181688565494	36.0	36.0	36.0	36.0	36.0
18	35.12403421870967	36.0	36.0	36.0	36.0	36.0
19	35.0828537715557	36.0	36.0	36.0	36.0	36.0
20	35.04179869467422	36.0	36.0	36.0	36.0	36.0
21	34.99146007202645	36.0	36.0	36.0	36.0	36.0
22	34.954672500891114	36.0	36.0	36.0	36.0	36.0
23	34.90835555992576	36.0	36.0	36.0	32.0	36.0
24	34.89580869971361	36.0	36.0	36.0	32.0	36.0
25	34.82805712951241	36.0	36.0	36.0	32.0	36.0
26	34.79064516525523	36.0	36.0	36.0	32.0	36.0
27	34.714654801558524	36.0	36.0	36.0	32.0	36.0
28	34.64414139800145	36.0	36.0	36.0	32.0	36.0
29	34.599840214358586	36.0	36.0	36.0	32.0	36.0
30	34.52638675499945	36.0	36.0	36.0	32.0	36.0
31	34.52569844762104	36.0	36.0	36.0	32.0	36.0
32	34.46434321955776	36.0	36.0	36.0	32.0	36.0
33	34.49098686070379	36.0	36.0	36.0	32.0	36.0
34	34.42423333620128	36.0	36.0	36.0	32.0	36.0
35	34.359725414520824	36.0	36.0	36.0	32.0	36.0
36	34.2813198293981	36.0	36.0	36.0	32.0	36.0
37	34.15917108125714	36.0	36.0	36.0	32.0	36.0
38	34.082271168524684	36.0	36.0	36.0	32.0	36.0
39	33.831222114332775	36.0	36.0	36.0	32.0	36.0
40	33.889065745645844	36.0	36.0	36.0	32.0	36.0
41	33.8609864919677	36.0	36.0	36.0	32.0	36.0
42	33.746624221044996	36.0	36.0	36.0	32.0	36.0
43	33.80386558340196	36.0	36.0	36.0	32.0	36.0
44	33.689081724209984	36.0	36.0	36.0	27.0	36.0
45	33.555208397350015	36.0	36.0	36.0	21.0	36.0
46	33.50856573950024	36.0	36.0	36.0	21.0	36.0
47	33.48912965990241	36.0	36.0	36.0	21.0	36.0
48	33.44586585380843	36.0	36.0	36.0	21.0	36.0
49	33.422156122862866	36.0	36.0	36.0	21.0	36.0
50	32.94307575068523	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	6.0
21	22.0
22	80.0
23	263.0
24	746.0
25	1825.0
26	4038.0
27	7830.0
28	12524.0
29	17965.0
30	25071.0
31	34318.0
32	49991.0
33	73660.0
34	150836.0
35	434412.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11265839590494	19.429774742214814	12.708059813047349	25.749507048832896
2	12.579834700991697	21.05042553923762	44.13857189916859	22.231167860602095
3	19.2267320234124	26.6516385483526	29.34662100871942	24.775008419515576
4	10.880541796236434	17.490259221475192	38.669477255128506	32.959721727159874
5	11.205767032534816	40.005653953465504	35.37690974569501	13.411669268304674
6	31.27978337797127	37.58473321099384	18.526075397347288	12.6094080136876
7	27.18116887027725	31.362518421463413	22.71245063539453	18.7438620728648
8	24.606620042035914	40.01720768446023	20.029253063582395	15.346919209921458
9	26.922036898191966	14.540493368895882	19.9650929829521	38.57237674996005
10	15.90936466770732	28.289801988716672	33.63598372644698	22.164849617129022
11	35.53706412320702	19.56722673582517	27.0490050271021	17.846704113865705
12	25.965904202362367	23.038754163645077	32.70037733993781	18.294964294054743
13	31.597241854005087	21.607320640617512	25.765557590432525	21.029879914944875
14	20.743371968682016	24.244521196179893	25.683944001278284	29.328162833859807
15	21.917058960901684	36.13539989429565	22.65403950392704	19.293501640875625
16	21.14713799333817	26.162440541304587	32.26821863592227	20.422202829434973
17	20.15351712779164	29.77064614855148	29.624012094543932	20.451824629112945
18	21.323516759055543	24.60514509765361	35.90850428348431	18.162833859806536
19	25.219336520852025	25.48396612544402	26.083039368723803	23.21365798498015
20	24.47006477464079	26.943177767671678	29.421575978072497	19.16518147961504
21	23.229390725058078	25.798620927002545	24.911687705109454	26.060300642829926
22	23.718703523887953	30.51918042257157	26.087832937966297	19.674283115574184
23	19.7911724578719	29.634951265379367	28.503791836182845	22.07008444056589
24	24.251527181995847	27.227964945488516	28.75305743679249	19.767450435723152
25	22.717953760493614	27.979449108273208	28.688036971939184	20.614560159293994
26	18.153000897257833	29.98893791713271	30.96719477869689	20.890866406912572
27	21.179340945685173	29.513514177902877	28.8049263142369	20.502218562175052
28	19.437800366277855	29.261913248687915	27.41835568283779	23.88193070219644
29	21.327572856106887	26.99578411730724	27.324327978465814	24.35231504812006
30	20.235622365073315	27.290035521577206	29.685345198441475	22.788996914908
31	23.362750279624873	25.625437874113498	28.042257156553056	22.969554689708577
32	20.60952076598778	29.467667990019542	28.2852542435379	21.637557000454773
33	19.14477808232648	29.14613011467693	30.893324647549747	20.815767155446846
34	20.765250310352883	29.4761489202178	30.81662753966986	18.941973229759462
35	21.152546122739952	29.72086677564867	29.5886134293686	19.537973672242774
36	19.114664634521073	29.17624356248233	30.665445740483538	21.043646062513062
37	21.024471785543085	28.884819134945122	26.85701643333866	23.233692646173136
38	21.14812128959304	30.969775931365923	26.52343317887388	21.35866960016716
39	23.485662311483672	26.472547597684336	27.857028724541845	22.184761366290147
40	25.61572782359665	26.734841873671012	28.472818004154426	19.176612298577908
41	22.999176489386546	25.309431040204526	31.39542029769294	20.295972172715988
42	23.212183040597843	30.339114295898423	28.886539903391146	17.56216276011259
43	19.701938322742414	29.633476320997058	29.582590739807518	21.081994616453002
44	19.13322435133175	29.9734510011185	28.964834867685198	21.928489779864552
45	19.866394621369484	30.387541636450795	28.204746862670387	21.541316879509335
46	20.661660863163096	26.332214422761368	29.300051991853383	23.706072722222153
47	24.472523015277968	26.23495864010128	28.52542435378999	20.767093990830762
48	20.488575326638724	29.490037979817846	26.479430671468428	23.541956022075
49	22.17320763529542	27.179414692904285	28.58110350422203	22.066274167578264
50	19.75429884831426	32.50236605661328	25.382932435256087	22.36040265981637
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	53.0
1	44.0
2	35.0
3	131.0
4	227.0
5	261.0
6	295.0
7	237.0
8	179.0
9	242.0
10	305.0
11	439.0
12	573.0
13	978.0
14	1383.0
15	1862.0
16	2341.0
17	2673.0
18	3005.0
19	3258.0
20	3511.0
21	4082.5
22	4654.0
23	5065.0
24	5476.0
25	6755.5
26	8035.0
27	12772.0
28	17509.0
29	22184.0
30	26859.0
31	32241.5
32	37624.0
33	48863.0
34	60102.0
35	70871.0
36	81640.0
37	80890.0
38	80140.0
39	70678.5
40	61217.0
41	54435.0
42	47653.0
43	41997.0
44	36341.0
45	38927.0
46	41513.0
47	44133.0
48	46753.0
49	44169.5
50	41586.0
51	35815.5
52	30045.0
53	27881.0
54	25717.0
55	26881.5
56	28046.0
57	28014.5
58	27983.0
59	25139.0
60	22295.0
61	19779.5
62	17264.0
63	15191.0
64	13118.0
65	11654.5
66	10191.0
67	9212.5
68	8234.0
69	8164.0
70	8094.0
71	6264.0
72	4434.0
73	4028.0
74	3622.0
75	2562.0
76	1502.0
77	1452.0
78	1402.0
79	1185.5
80	969.0
81	790.5
82	612.0
83	539.5
84	467.0
85	411.5
86	356.0
87	250.5
88	145.0
89	97.5
90	50.0
91	35.5
92	21.0
93	12.5
94	4.0
95	4.0
96	4.0
97	4.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014995267886773437
2	0.004179009083199154
3	4.916481274351947E-4
4	0.0
5	0.0
6	8.603842230115906E-4
7	1.2291203185879867E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.2291203185879867E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	813590.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.812123886575414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.09637947921165	38.6086227411886
2	12.335771554385754	12.042704186983409
3	3.6765985609594347	5.383877533162704
4	1.4975416113797273	2.923927466398761
5	0.7831276126844817	1.9113061024676483
6	0.4841908819413179	1.418063118844189
7	0.3084843962836569	1.0540444997931302
8	0.21864935212151276	0.8538191410779785
9	0.1734212383585096	0.7618553074187004
>10	1.1181977911097853	10.830753250387842
>50	0.15761382239193653	5.417530317026357
>100	0.1368681640004935	13.237420302563226
>500	0.011131606683781116	3.6355547297812825
>1k	0.002023928487960203	1.9205213029061992
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2574	0.31637557000454775	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2292	0.28171437702036656	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	2105	0.25872982706277114	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2006	0.2465615359087501	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	2005	0.24643862387689128	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	1978	0.24311999901670375	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1420	0.1745350852394941	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	1172	0.14405290133851203	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	979	0.12033087918976387	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	899	0.11049791664106	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	891	0.10951462038618962	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	835	0.10263154660209689	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	833	0.10238572253837927	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	828	0.10177116237908529	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	823	0.1011566022197913	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	2.458240637175973E-4	0.0
14	0.0	0.0	0.0	2.458240637175973E-4	0.0
15	0.0	0.0	0.0	2.458240637175973E-4	0.0
16	0.0	0.0	0.0	4.916481274351946E-4	0.0
17	0.0	0.0	0.0	4.916481274351946E-4	0.0
18	0.0	0.0	0.0	9.832962548703892E-4	0.0
19	0.0	0.0	0.0	0.001106208286729188	0.0
20	0.0	0.0	0.0	0.0017207684460231812	0.0
21	0.0	0.0	0.0	0.0025811526690347718	0.0
22	0.0	0.0	0.0	0.003933185019481557	0.0
23	0.0	0.0	0.0	0.007743458007104315	0.0
24	0.0	0.0	0.0	0.010816258803574282	0.0
25	0.0	0.0	0.0	0.013028675377032659	0.0
26	0.0	0.0	0.0	0.016838948364655416	0.0
27	0.0	1.2291203185879864E-4	0.0	0.021632517607148564	0.0
28	0.0	1.2291203185879864E-4	0.0	0.02851559139124129	0.0
29	0.0	1.2291203185879864E-4	0.0	0.037856905812509986	0.0
30	0.0	1.2291203185879864E-4	0.0	0.05899777529222336	0.0
31	1.2291203185879864E-4	1.2291203185879864E-4	0.0	0.09316732014896938	0.0
32	1.2291203185879864E-4	1.2291203185879864E-4	0.0	0.13901350803230128	0.0
33	2.458240637175973E-4	1.2291203185879864E-4	0.0	0.181909807151022	0.0
34	2.458240637175973E-4	1.2291203185879864E-4	0.0	0.23119753192640027	0.0
35	2.458240637175973E-4	1.2291203185879864E-4	0.0	0.288720362836318	0.0
36	2.458240637175973E-4	1.2291203185879864E-4	0.0	0.3651716466524908	0.0
37	2.458240637175973E-4	1.2291203185879864E-4	0.0	0.4837817573962315	0.0
38	2.458240637175973E-4	1.2291203185879864E-4	0.0	0.6039897245541366	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGCAC	40	7.0303725E-4	27.499846	7
AATCGCG	40	7.0303725E-4	27.499846	19
ACGCACC	45	0.0013978665	24.444307	8
CGAGCAA	45	0.0013978665	24.444307	21
CGCACCG	45	0.0013978665	24.444307	9
TATAAGG	45	0.0013978665	24.444307	2
AGCGCAA	155	0.0	24.128899	42
GTCCTAG	120	2.3646862E-11	23.839062	1
CTAGGAC	215	0.0	23.534752	3
CGCAATC	155	0.0	22.70955	44
GATATAC	990	0.0	22.672113	1
ATACCGT	215	0.0	22.511503	6
ACTGTTC	960	0.0	22.458208	8
TGTAGGA	3325	0.0	22.231457	2
CTGTAGG	3300	0.0	22.005287	1
CAGCGCA	160	0.0	21.999876	41
ACCGGAT	50	0.0025798033	21.999876	12
CGACGTG	50	0.0025798033	21.999876	6
GTCGTAG	210	0.0	21.999876	11
GACGGTT	70	3.213827E-5	21.999876	21
>>END_MODULE