FastQCFastQC Report
Thu 2 Feb 2017
SRR4062357_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062357_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGG251.5615240474703311No Hit
GTATCAACGCAGAGTACTTTTTTTT201.2492192379762648No Hit
TATCAACGCAGAGTACTTTTTTTTT140.8744534665833853No Hit
GTACTTTTTTTTTTTTTTTTTTTTT110.6870705808869456No Hit
GGTATCAACGCAGAGTACTTTTTTT80.49968769519050593No Hit
ACGCAGAGTACTTTTTTTTTTTTTT80.49968769519050593No Hit
GTATCAACGCAGAGTACATGGGGTG70.43722673329169265No Hit
GTATCAACGCAGAGTACATGGGAAG70.43722673329169265No Hit
CATGTACTCTGCGTTGATACCACTG70.43722673329169265No Hit
TCCATGTACTCTGCGTTGATACCAC60.3747657713928795No Hit
GTATCAACGCAGAGTACATGGAAGC60.3747657713928795No Hit
GTACATGGAAGCAGTGGTATCAACG60.3747657713928795No Hit
GCAGAGTACTTTTTTTTTTTTTTTT50.3123048094940662No Hit
GAGTACATGGAAGCAGTGGTATCAA50.3123048094940662No Hit
ATCAACGCAGAGTACTTTTTTTTTT50.3123048094940662No Hit
GAGTACTTTTTTTTTTTTTTTTTTT50.3123048094940662No Hit
GTACATGGGGTGGTATCAACGCAAA40.24984384759525297No Hit
ATCAGATACCGTCGTAGTTCCGACC40.24984384759525297No Hit
GTATCAACGCAGAGTACATGGGTGG40.24984384759525297No Hit
ATCAACGCAGAGTACATGGAAGCAG30.18738288569643974No Hit
CCACTGCTTCCATGTACTCTGCGTT30.18738288569643974No Hit
ATGGGAAGCAGTGGTATCAACGCAG30.18738288569643974No Hit
GGTATCAACGCAGAGTACATGGGAG30.18738288569643974No Hit
GTACATGGGGTGGTATCAACGCAGA30.18738288569643974No Hit
GGTCCAAGAATTTCACCTCTAGCGG30.18738288569643974No Hit
GTATCAACGCAAAAAAAAAAAAAAA30.18738288569643974No Hit
TATCAACGCAGAGTACATGGGTGGT30.18738288569643974No Hit
TCCCCGCCCCTTGCCTCTCGGCGCC30.18738288569643974No Hit
GCGTTGATACCACTGCTTCCATGTA30.18738288569643974No Hit
GGCCTGGTCCGCGTTCGCTCGCCAC30.18738288569643974No Hit
GCTTCCATGTACTCTGCGTTGATAC30.18738288569643974No Hit
GTCTTGCGCCGGTCCAAGAATTTCA30.18738288569643974No Hit
GAATAGGACCGCGGTTCTATTTTGT30.18738288569643974No Hit
GTACATGGGAAGCAGTGGTATCAAC30.18738288569643974No Hit
GCTAAAGACCGTGGTGATTTTATAG30.18738288569643974No Hit
GTTCCGAAAACCAACAAAATAGAAC30.18738288569643974No Hit
CCTTTAAAAACACTGATTTGTTCTT20.12492192379762648No Hit
GCTGTGGCTCAGCTTGCCAATTTGT20.12492192379762648No Hit
AAAAAAAGTACTCTGCGTTGATACC20.12492192379762648No Hit
GTGCGGCTACATCCACCTATGAAGT20.12492192379762648No Hit
CGTTCGAATGGGTCGTCGCCGCCAC20.12492192379762648No Hit
ATCACGGAAAATGAGGAATACACAA20.12492192379762648No Hit
GCTCTGACGGCTCCCGTCGCAGGAT20.12492192379762648No Hit
GTATCTGATCGTCTTCGAACCTCCG20.12492192379762648No Hit
TATCAACGCAGAGTACATGGGAAGC20.12492192379762648No Hit
GATACCACTGCTTCCATGTACTCTG20.12492192379762648No Hit
ACGCAGAGTACATGGGTGGTATCAA20.12492192379762648No Hit
AGATTTCATGTAGCGCTCTGCACCC20.12492192379762648No Hit
GTTCCGACCATAAACGATGCCGACT20.12492192379762648No Hit
CACTTCTTCCTCAGTTGGTTTCCTT20.12492192379762648No Hit
GTACATGGGGAATAATTGCAATCCC20.12492192379762648No Hit
TAACAATACAGGACTCTTTCGAGGC20.12492192379762648No Hit
GTACATGGGAGTGGTATCAACGCAA20.12492192379762648No Hit
GTATCAACGCAGAGTACATGGGGTA20.12492192379762648No Hit
AATCATGGCCTCAGTTCCGAAAACC20.12492192379762648No Hit
ATAGCGTTGCTGTTGTGAACATTTA20.12492192379762648No Hit
CTTTGCACAATGCTTGGAACAAGTA20.12492192379762648No Hit
CGACGCCCCGGTGATTTACGGGCTG20.12492192379762648No Hit
AAGAACGGCCATGCACCACCACCCA20.12492192379762648No Hit
GTCAGCTCTCCTGTCTGCCCCTCCT20.12492192379762648No Hit
GCAGAATTTGGCTGTTTGGTCTCAA20.12492192379762648No Hit
GGTCATGGGAATAACGCCGCCGCAT20.12492192379762648No Hit
GGCGGGCGGTCCGCCGCGAGGCGAG20.12492192379762648No Hit
CTCCCCGGGTCGGGAGTGGGTAATT20.12492192379762648No Hit
ATACCACTGCTTCCATGTACTCTGC20.12492192379762648No Hit
AAGCAGGCCCGAGCCGCCTGGATAC20.12492192379762648No Hit
AGAGTGGACCCTGCGGGTTCGTCAC20.12492192379762648No Hit
GTCTAAGGGAGTTTTAGATCAAAAA20.12492192379762648No Hit
GGCTTACAGAACGCTCTCCTACCAT20.12492192379762648No Hit
GTGGTGCATGGCCGTTCTTAGTTGG20.12492192379762648No Hit
CCCATTAGTCCTCTCATCATGTGCT20.12492192379762648No Hit
GAGTCAAGCAATGATGCAAGGCTGG20.12492192379762648No Hit
GGTATCAACGCAGAGTACATGGGGT20.12492192379762648No Hit
CAAGAACCCCGCCTGTTTACCAAAA20.12492192379762648No Hit
CTCCAGAGCTTATTTTTGGTGCTAC20.12492192379762648No Hit
ACGCAGAGTACATGGAAGCAGTGGT20.12492192379762648No Hit
ACGCAGAGTACATGGGGGTATCAAC20.12492192379762648No Hit
CTGCGTTGCCCATGTACTCTGCGTT20.12492192379762648No Hit
GCCAGGCGCAGCCAGGAGCCATTGC20.12492192379762648No Hit
AAATAGAACCGCGGTCCTATTCCAT20.12492192379762648No Hit
GATTTGTCTGGTTAATTCCGATAAC20.12492192379762648No Hit
GATACCACTGCTTCCCATGTACTCT20.12492192379762648No Hit
GGAGAGGAGCGAGCGACCAAAGGAA20.12492192379762648No Hit
TTTTGCCTTTTGGCTGCATCTTTAT20.12492192379762648No Hit
GTACATGGGGTATCAACGCAAAAAA20.12492192379762648No Hit
CATGGAAGACAACTCTTATGGACCC20.12492192379762648No Hit
CTAGATAACCTCGGGCCGATCGCAC20.12492192379762648No Hit
GTTATAATCATTCGGAGGATTTTTT20.12492192379762648No Hit
GCTTTGAACACTCTAATTTTTTCAA20.12492192379762648No Hit
GCGCAATCCTATTTAAGAGTTCATA20.12492192379762648No Hit
GAATTCCTCGTTCATGGGGAATAAT20.12492192379762648No Hit
AACCAGCAGCTTGTCAGGGAGGGTT20.12492192379762648No Hit
GGCTGGTTGAGAACAACATCACTGG20.12492192379762648No Hit
GTGGTATCAACGCAGAGTACATGGA20.12492192379762648No Hit
ACCTTGGGGACCTGCTGCGGTTATG20.12492192379762648No Hit
CATGGAAGCAGTGGTATCAACGCAG20.12492192379762648No Hit
GGTATCAACGCAGAGTACATGGAAG20.12492192379762648No Hit
ACCATACTCCCCCCGGAACCCAAAG20.12492192379762648No Hit
ACCCCCGAGCGGTCGGCGTCCCCCA20.12492192379762648No Hit
GGACCTGCGCGCCGCCAACATCCTG20.12492192379762648No Hit
GCGTTGATACCACTGCTTCCCATGT20.12492192379762648No Hit
CCTCTGTGTGCTTCCAAGTCACATA20.12492192379762648No Hit
GGCCTCGGTGGTGCTCCTGATGTCC20.12492192379762648No Hit
CTTCCATGTACTCTGCGTTGATACC20.12492192379762648No Hit
GGGCAGACGTGTTCCTTCCCCTCTA20.12492192379762648No Hit
TATCAACGCAGAGTACATGGGGTGG20.12492192379762648No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA20.12492192379762648No Hit
CGGGTTACCCGCGCCTGCCGGCGTA20.12492192379762648No Hit
GTACATGGGACATGATTCAGGCTGG20.12492192379762648No Hit
GTGCAGTTGCCCTGAAAGTTGTGTT20.12492192379762648No Hit
TATCAACGCAAAAAAAAAAAAAAAA20.12492192379762648No Hit
CTCCCAAGATCCAACTACGAGCTTT20.12492192379762648No Hit
GCTCAGGATTGACACCTAAGAACGG20.12492192379762648No Hit
GGTCGGAACTACGACGGTATCTGAT20.12492192379762648No Hit
GTCCAAGAATTTCACCTCTAGCGGC20.12492192379762648No Hit
GTACATGGGTGGTATCAACGCAAAA20.12492192379762648No Hit
AGACAGGGCAGGGCTGGGAAGCAGG20.12492192379762648No Hit
TCTCAGGCTCCCTCTCCGGAATCGA20.12492192379762648No Hit
GCGTGTGGAAGCCTTCCAGCTTGGT20.12492192379762648No Hit
GAGTGGTATCAACGCAAAAAAAAAA20.12492192379762648No Hit
ACATGGGAAGCAGTGGTATCAACGC20.12492192379762648No Hit
CGCAAAAAAAAAAAAAAAAAAAAAA20.12492192379762648No Hit
TTCAAAAGCTTATTCACTTCTCTTT20.12492192379762648No Hit
CTCCAGGTAGCGGCGATCTTCTGCT20.12492192379762648No Hit
TAGAGGCACTGCCTGCCCAGTGACT20.12492192379762648No Hit
TTTCCACATCTCTCCCTTTGGTTCA20.12492192379762648No Hit
CAAGTGTACAGCTCAGGAACAGAGC20.12492192379762648No Hit
CATCAACATCGTCGTAATCGGACAC20.12492192379762648No Hit
ACCTCGGGCCGATCGCACGCCCCCC20.12492192379762648No Hit
CACATGAAGTGCTCTGTCATATCTT20.12492192379762648No Hit
GAGCTGCAGGAGACTCCAGGGGAGA20.12492192379762648No Hit
TAGGGAGGCAGCAGAAGAAGAAGGA20.12492192379762648No Hit
CCTGTACAGCCTGCAGAACCATGAG20.12492192379762648No Hit
CTCTAATTTTTTCAAAGTAAACGCT20.12492192379762648No Hit
GAGTACATGGGAATTTAATTGTGTT20.12492192379762648No Hit
GTACTCTGCGTTGATACCACTGCCC20.12492192379762648No Hit
ACGCAGAGTACATGGGAAGCAGTGG20.12492192379762648No Hit
CCATCCTGGGGTTCTCACTGAATTG20.12492192379762648No Hit
TTTCAGGTGCTGCTTTATTTGCTCG20.12492192379762648No Hit
CTTCAAGGCTGACTCCAGGCTCTTC20.12492192379762648No Hit
ACGCAGAGTACATGGGGTGGTATCA20.12492192379762648No Hit
GTCCTATTCCATTATTCCTAGCTGC20.12492192379762648No Hit
GGGCTGTCGAGATGACGACGGCTTC20.12492192379762648No Hit
GCTCTCCTACTCTAGTCCCACGTTT20.12492192379762648No Hit
CTTTCTGACCATCGCAATGCTATGT20.12492192379762648No Hit
ACACTTCGCTCCACTTTCCAGCAGA20.12492192379762648No Hit
GTTGTGAGCCACCATGTGGTTGCTG20.12492192379762648No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)