FastQCFastQC Report
Thu 2 Feb 2017
SRR4062356_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062356_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2822203
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT272560.9657703574122769No Hit
GTCCTACAGTGGACATTTCTAAATT249320.8834233398518817No Hit
CTGTAGGACGTGGAATATGGCAAGA229570.8134425482504269No Hit
CTTTAGGACGTGAAATATGGCGAGG217140.7693989411817648No Hit
GTCCTACAGTGTGCATTTCTCATTT149400.5293736843168263No Hit
CTGTAGGACCTGGAATATGGCGAGA123510.437636839022565No Hit
CTGAAGGACCTGGAATATGGCGAGA117660.4169083513836531No Hit
GTACATGGAAGCAGTGGTATCAACG111230.39412473163695166No Hit
ATTTAGAAATGTCCACTGTAGGACG105440.3736088438712594No Hit
GTCCTTCAGTGTGCATTTCTCATTT101070.35812448643843126No Hit
TCCATGTACTCTGCGTTGATACCAC91920.3257030057724409No Hit
TTTCTAAATTTTCCACCTTTTTCAG85170.30178552003523484No Hit
GAATATGGCAAGAAAACTGAAAATC64910.2299976295114136No Hit
GGAATATGGCGAGAAAACTGAAAAT61360.2174188036792534No Hit
CTGTAGGACATGGAATATGGCAAGA51970.18414692352038461No Hit
ATTCCAGGTCCTTCAGTGTGCATTT51890.18386345702275847No Hit
GATATACACTGTTCTACAAATCCCG51030.1808161921732774No Hit
GCCATATTCCACGTCCTACAGTGGA50450.17876106006548786No Hit
TTGTAGAACAGTGTATATCAATGAG48890.173233463361778No Hit
GAAATATGGCGAGGAAAACTGAAAA48670.17245393049330612No Hit
ACCTGGAATATGGCGAGAAAACTGA48480.171780697561444No Hit
ACAGTGGACATTTCTAAATTTTCCA48030.17018619851229697No Hit
GTCCACTGTAGGACGTGGAATATGG47140.16703263372620608No Hit
CCATATTCCAGGTCCTTCAGTGTGC45870.16253260307639103No Hit
GACCTGGAATATGGCGAGAAAACTG45510.16125700383707336No Hit
ATACACACTTTAGGACGTGAAATAT44130.15636720675302238No Hit
CCATATTTCACGTCCTAAAGTGTGT44010.15594200700658314No Hit
TTCCAGGTCCTTCAGTGTGCATTTC42590.15091047667371907No Hit
GAGTACATGGAAGCAGTGGTATCAA42330.1499892105564341No Hit
GTATCAACGCAGAGTACTTTTTTTT41190.14594981296526152No Hit
CCTAAAGTGTGTATTTCTCATTTTC40250.14261908161815434No Hit
CCACTGTAGGACGTGGAATATGGCA39660.1405285161981615No Hit
CACTTTAGGACGTGAAATATGGCGA39500.13996158320290922No Hit
CTACAGTGGACATTTCTAAATTTTC38460.13627651873376934No Hit
ATTCCACGTCCTACAGTGGACATTT37580.13315838725988174No Hit
GTGTATTTCTCATTTTCCGTGATTT37180.131741054771751No Hit
GAAATACACACTTTAGGACGTGAAA36860.1306071887812464No Hit
AGTGTGTATTTCTCATTTTCCGTGA34620.12267012684771435No Hit
GTGTATATCAATGAGTTACAATGAA34470.12213862716466534No Hit
CATGTACTCTGCGTTGATACCACTG33930.12022522830568885No Hit
TCCTAAAGTGTGTATTTCTCATTTT33450.11852442931993198No Hit
GTAGGACGTGGAATATGGCAAGAAA33170.11753229657824046No Hit
GTGTATATCAATGAGTTACAATGAG31900.11303226592842541No Hit
GGTCCTTCAGTGTGCATTTCTCATT30350.10754010253691884No Hit
GTTCTACAGTGTGGTTTTTATCATT29910.10598103679997505No Hit
TATCAACGCAGAGTACTTTTTTTTT29390.10413850456540512No Hit
GTCCTACAGTGGACATTTCTAAATA29020.10282747201388417No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAC250.00602575919.00409918
TGTCCGC607.683593E-817.4182610
GACGTTA551.12854905E-515.546057
CGACTGA456.762784E-414.77834314
ACGCCCC1400.014.2528217
CGTACCG400.00527420514.25256816
TATTACG400.00529472314.2442292
ACGCGCG759.678515E-713.93361913
TGACGAG903.632522E-813.72518318
CCGCATC1550.013.48606212
GCGCATT500.001498841413.30145411
TAGGACC45000.013.1290524
CCAACGA5700.013.00234219
CTCGTAG957.3861884E-813.00118910
GCTCGTA957.3878255E-813.0009589
GCATCGC1700.012.85343314
TCGCCAG2000.012.827538517
TCGCCGC604.0876435E-412.6689516
AAATGTC31150.012.5654897
TCCAACG7800.012.54757818
Produced by FastQC (version 0.11.5)