##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062356_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2822203
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30891293078492	32.0	32.0	32.0	32.0	32.0
2	31.054328125935662	32.0	32.0	32.0	32.0	32.0
3	31.02660120480348	32.0	32.0	32.0	32.0	32.0
4	31.085003452976274	32.0	32.0	32.0	32.0	32.0
5	30.990948914730797	32.0	32.0	32.0	32.0	32.0
6	34.65779428340201	36.0	36.0	36.0	32.0	36.0
7	34.55067902627841	36.0	36.0	36.0	32.0	36.0
8	34.56874540917149	36.0	36.0	36.0	32.0	36.0
9	34.64402348094733	36.0	36.0	36.0	32.0	36.0
10	34.392656020846125	36.0	36.0	36.0	32.0	36.0
11	34.63241411053705	36.0	36.0	36.0	32.0	36.0
12	34.46031274150017	36.0	36.0	36.0	32.0	36.0
13	34.52900553220304	36.0	36.0	36.0	32.0	36.0
14	34.464457376028584	36.0	36.0	36.0	32.0	36.0
15	34.42585809737995	36.0	36.0	36.0	32.0	36.0
16	34.45626802891216	36.0	36.0	36.0	32.0	36.0
17	34.423233197611935	36.0	36.0	36.0	32.0	36.0
18	34.44487373870696	36.0	36.0	36.0	32.0	36.0
19	34.33824710695864	36.0	36.0	36.0	32.0	36.0
20	34.32862235636487	36.0	36.0	36.0	32.0	36.0
21	34.26206265105664	36.0	36.0	36.0	32.0	36.0
22	34.194671325911	36.0	36.0	36.0	32.0	36.0
23	34.209147959944765	36.0	36.0	36.0	32.0	36.0
24	34.14643312334371	36.0	36.0	36.0	32.0	36.0
25	33.73622450263146	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	103.0
5	263.0
6	471.0
7	112.0
8	420.0
9	536.0
10	358.0
11	78.0
12	228.0
13	182.0
14	446.0
15	471.0
16	766.0
17	1069.0
18	1348.0
19	1747.0
20	2751.0
21	3993.0
22	6027.0
23	9040.0
24	12766.0
25	18244.0
26	26207.0
27	34069.0
28	46706.0
29	63577.0
30	83932.0
31	116905.0
32	170836.0
33	253389.0
34	607427.0
35	1357725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.84421047110994	20.602776637913745	12.931869677041675	25.621143213934644
2	11.1390335505118	20.770999499309557	48.72834194924849	19.361625000930157
3	18.37129252115294	27.279517913471903	30.270978779376527	24.07821078599863
4	10.399070657857573	17.863434476311237	40.22888381486728	31.508611050963914
5	9.945080574956117	40.974024040629175	37.149832838512644	11.931062545902064
6	28.974028294355453	39.412162574142585	19.7615597203178	11.852249411184168
7	25.911999191579664	32.24792266112119	23.93955994277235	17.9005182045268
8	21.89211762473645	44.472832572894816	20.395865436961035	13.239184365407702
9	26.60292321808651	15.504852581747993	21.73629606299436	36.15592813717113
10	15.246184170195376	29.86202561104363	35.79770567734172	19.094084541419278
11	32.88089954607619	20.14502856757682	30.044044267770698	16.930027618576286
12	25.97411306374871	23.59397674575381	34.75693742216356	15.67497276833392
13	30.514883266825983	23.979542603493197	26.497586152090868	19.007987977589956
14	18.33657661748819	26.450617229200557	27.47607449678835	27.736731656522906
15	19.195048087036632	41.10550343017669	22.46225634996848	17.2371921328182
16	17.622450405785358	27.942041406495544	36.135400843851926	18.300107343867175
17	17.976898668611764	32.85419014074513	31.79555059190155	17.373360598741552
18	18.533123056372084	26.495180955861354	39.373178333903965	15.598517653862597
19	24.021803458068472	26.172381151071583	27.685055484358077	22.120759906501867
20	23.641062082280406	28.985531411874938	31.019002549732555	16.354403956112105
21	20.263075544277715	27.511951367142736	26.599943374255723	25.625029714323826
22	22.4439635501356	34.060033400783134	27.147398521876948	16.34860452720432
23	17.066683320228115	33.34322138544384	31.01930441101747	18.570790883310575
24	23.166885825941883	27.920879835194118	31.9291882543875	16.9830460844765
25	20.052000175983128	30.699121361655262	31.597628769267672	17.65124969309394
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	360.0
1	360.0
2	881.5
3	1403.0
4	1403.0
5	1403.0
6	3060.5
7	4718.0
8	4718.0
9	4718.0
10	5622.0
11	6526.0
12	6526.0
13	6526.0
14	9040.5
15	11555.0
16	11555.0
17	11555.0
18	21542.0
19	31529.0
20	31529.0
21	31529.0
22	61536.0
23	91543.0
24	91543.0
25	91543.0
26	151847.0
27	212151.0
28	212151.0
29	212151.0
30	293218.0
31	374285.0
32	374285.0
33	374285.0
34	391952.0
35	409619.0
36	409619.0
37	409619.0
38	403531.0
39	397443.0
40	397443.0
41	397443.0
42	388961.5
43	380480.0
44	380480.0
45	380480.0
46	383451.5
47	386423.0
48	386423.0
49	386423.0
50	314284.5
51	242146.0
52	242146.0
53	242146.0
54	171979.0
55	101812.0
56	101812.0
57	101812.0
58	81907.0
59	62002.0
60	62002.0
61	62002.0
62	52803.5
63	43605.0
64	43605.0
65	43605.0
66	35634.0
67	27663.0
68	27663.0
69	27663.0
70	20828.0
71	13993.0
72	13993.0
73	13993.0
74	11505.5
75	9018.0
76	9018.0
77	9018.0
78	8257.0
79	7496.0
80	7496.0
81	7496.0
82	5281.0
83	3066.0
84	3066.0
85	3066.0
86	2350.0
87	1634.0
88	1634.0
89	1634.0
90	1137.0
91	640.0
92	640.0
93	640.0
94	400.0
95	160.0
96	160.0
97	160.0
98	546.5
99	933.0
100	933.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008114228494548409
2	0.003543331220326816
3	0.004854363771847737
4	0.014952857749779162
5	0.03468921264699953
6	0.04560267280560612
7	0.06689809343977028
8	0.09063841261595995
9	0.0983628746762724
10	0.12114649442297382
11	0.12217406047686862
12	0.13439855318699612
13	0.12284729340873071
14	0.1260717248192281
15	0.11522913128502804
16	0.11427243185553981
17	0.12369769290160913
18	0.13103238852768564
19	0.13291035407445886
20	0.12316619321856011
21	0.13099695521548238
22	0.1525404090350694
23	0.14127261575443015
24	0.13666628516800527
25	0.13291035407445886
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2822203.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.05965074412312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.67834992767095	32.31946520714649
2	11.726115477611595	9.394881812367538
3	3.0753905294370085	3.6959721153309135
4	1.2362331856121374	1.9809227861566783
5	0.619925758538548	1.2417004687169917
6	0.422155529067866	1.014684183249554
7	0.28666379403741993	0.8038556029087001
8	0.21582069998567482	0.6916561491822648
9	0.1698691003071185	0.6124407147469425
>10	1.2449906733038343	9.987577464353933
>50	0.156256936623158	4.349289420743719
>100	0.13674769103559592	11.094647581506404
>500	0.017862642548689186	4.92146506350713
>1k	0.012026333597137256	10.149102506747008
>5k	7.074313880668974E-4	1.8020350522972703
>10k+	8.842892350836219E-4	5.940303871038516
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	27256	0.9657703574122769	No Hit
GTCCTACAGTGGACATTTCTAAATT	24932	0.8834233398518817	No Hit
CTGTAGGACGTGGAATATGGCAAGA	22957	0.8134425482504269	No Hit
CTTTAGGACGTGAAATATGGCGAGG	21714	0.7693989411817648	No Hit
GTCCTACAGTGTGCATTTCTCATTT	14940	0.5293736843168263	No Hit
CTGTAGGACCTGGAATATGGCGAGA	12351	0.437636839022565	No Hit
CTGAAGGACCTGGAATATGGCGAGA	11766	0.4169083513836531	No Hit
GTACATGGAAGCAGTGGTATCAACG	11123	0.39412473163695166	No Hit
ATTTAGAAATGTCCACTGTAGGACG	10544	0.3736088438712594	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	10107	0.35812448643843126	No Hit
TCCATGTACTCTGCGTTGATACCAC	9192	0.3257030057724409	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	8517	0.30178552003523484	No Hit
GAATATGGCAAGAAAACTGAAAATC	6491	0.2299976295114136	No Hit
GGAATATGGCGAGAAAACTGAAAAT	6136	0.2174188036792534	No Hit
CTGTAGGACATGGAATATGGCAAGA	5197	0.18414692352038461	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	5189	0.18386345702275847	No Hit
GATATACACTGTTCTACAAATCCCG	5103	0.1808161921732774	No Hit
GCCATATTCCACGTCCTACAGTGGA	5045	0.17876106006548786	No Hit
TTGTAGAACAGTGTATATCAATGAG	4889	0.173233463361778	No Hit
GAAATATGGCGAGGAAAACTGAAAA	4867	0.17245393049330612	No Hit
ACCTGGAATATGGCGAGAAAACTGA	4848	0.171780697561444	No Hit
ACAGTGGACATTTCTAAATTTTCCA	4803	0.17018619851229697	No Hit
GTCCACTGTAGGACGTGGAATATGG	4714	0.16703263372620608	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	4587	0.16253260307639103	No Hit
GACCTGGAATATGGCGAGAAAACTG	4551	0.16125700383707336	No Hit
ATACACACTTTAGGACGTGAAATAT	4413	0.15636720675302238	No Hit
CCATATTTCACGTCCTAAAGTGTGT	4401	0.15594200700658314	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	4259	0.15091047667371907	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4233	0.1499892105564341	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4119	0.14594981296526152	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	4025	0.14261908161815434	No Hit
CCACTGTAGGACGTGGAATATGGCA	3966	0.1405285161981615	No Hit
CACTTTAGGACGTGAAATATGGCGA	3950	0.13996158320290922	No Hit
CTACAGTGGACATTTCTAAATTTTC	3846	0.13627651873376934	No Hit
ATTCCACGTCCTACAGTGGACATTT	3758	0.13315838725988174	No Hit
GTGTATTTCTCATTTTCCGTGATTT	3718	0.131741054771751	No Hit
GAAATACACACTTTAGGACGTGAAA	3686	0.1306071887812464	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	3462	0.12267012684771435	No Hit
GTGTATATCAATGAGTTACAATGAA	3447	0.12213862716466534	No Hit
CATGTACTCTGCGTTGATACCACTG	3393	0.12022522830568885	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	3345	0.11852442931993198	No Hit
GTAGGACGTGGAATATGGCAAGAAA	3317	0.11753229657824046	No Hit
GTGTATATCAATGAGTTACAATGAG	3190	0.11303226592842541	No Hit
GGTCCTTCAGTGTGCATTTCTCATT	3035	0.10754010253691884	No Hit
GTTCTACAGTGTGGTTTTTATCATT	2991	0.10598103679997505	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2939	0.10413850456540512	No Hit
GTCCTACAGTGGACATTTCTAAATA	2902	0.10282747201388417	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.543331220326816E-5	0.0
6	0.0	0.0	0.0	3.543331220326816E-5	0.0
7	0.0	0.0	0.0	3.543331220326816E-5	0.0
8	0.0	0.0	0.0	3.543331220326816E-5	0.0
9	0.0	0.0	0.0	7.086662440653632E-5	0.0
10	0.0	0.0	0.0	7.086662440653632E-5	0.0
11	0.0	0.0	0.0	7.086662440653632E-5	0.0
12	0.0	0.0	0.0	7.086662440653632E-5	0.0
13	0.0	0.0	0.0	7.086662440653632E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTTAC	25	0.006025759	19.004099	18
TGTCCGC	60	7.683593E-8	17.41826	10
GACGTTA	55	1.12854905E-5	15.54605	7
CGACTGA	45	6.762784E-4	14.778343	14
ACGCCCC	140	0.0	14.25282	17
CGTACCG	40	0.005274205	14.252568	16
TATTACG	40	0.005294723	14.244229	2
ACGCGCG	75	9.678515E-7	13.933619	13
TGACGAG	90	3.632522E-8	13.725183	18
CCGCATC	155	0.0	13.486062	12
GCGCATT	50	0.0014988414	13.301454	11
TAGGACC	4500	0.0	13.129052	4
CCAACGA	570	0.0	13.002342	19
CTCGTAG	95	7.3861884E-8	13.001189	10
GCTCGTA	95	7.3878255E-8	13.000958	9
GCATCGC	170	0.0	12.853433	14
TCGCCAG	200	0.0	12.8275385	17
TCGCCGC	60	4.0876435E-4	12.66895	16
AAATGTC	3115	0.0	12.565489	7
TCCAACG	780	0.0	12.547578	18
>>END_MODULE