FastQCFastQC Report
Thu 2 Feb 2017
SRR4062356_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062356_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2822203
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA122880.43540454035375914No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA104980.37197891150990914No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA98520.3490889918265979No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC97320.3448369943622057No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA63980.22670233147650967No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA52690.18669812199901992No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA41530.14715454558017266No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT39870.14127261575443015No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA39830.14113088250561706No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA36880.13067805540565297No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA36630.12979222260057127No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC35890.1271701574975294No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG29040.10289833863829073No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT28240.10006367366202928No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCT300.005746466629.33315733
GGACGCG407.034723E-427.4998346
TAGGACC55500.025.6070544
TCGGACG450.001398725124.4442964
ACCGTCG2400.022.9165298
TACCGTC2450.022.4488457
GACCGTT602.8742116E-421.9998667
AGGACCT103100.021.4023915
CCGTCGT2600.021.1537199
TGTAGGA154200.021.0019132
GGACCTG103600.020.9593336
ATACCGT2550.020.7057576
CTGTAGG151850.020.6934781
AACCGGA755.476208E-520.53320942
TGAGCGG654.934606E-420.3075729
GACGTGT1750.020.1141627
TTAGGAC101350.020.1005213
GTAGGAC157650.020.0112973
AGGACGC550.004485226219.9998785
CGCGAGA550.004485226219.99987819
Produced by FastQC (version 0.11.5)