##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062356_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2822203
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33201155267711	32.0	32.0	32.0	32.0	32.0
2	31.53239047651781	32.0	32.0	32.0	32.0	32.0
3	31.594866138261494	32.0	32.0	32.0	32.0	32.0
4	31.669588261368865	32.0	32.0	32.0	32.0	32.0
5	31.645936525473186	32.0	32.0	32.0	32.0	32.0
6	35.270081918274485	36.0	36.0	36.0	36.0	36.0
7	35.29227344737427	36.0	36.0	36.0	36.0	36.0
8	35.22910152104579	36.0	36.0	36.0	36.0	36.0
9	35.33814647635199	36.0	36.0	36.0	36.0	36.0
10	35.26216505332891	36.0	36.0	36.0	36.0	36.0
11	35.34060342222016	36.0	36.0	36.0	36.0	36.0
12	35.2918542712909	36.0	36.0	36.0	36.0	36.0
13	35.30653641853545	36.0	36.0	36.0	36.0	36.0
14	35.2798473391177	36.0	36.0	36.0	36.0	36.0
15	35.23846725412736	36.0	36.0	36.0	36.0	36.0
16	35.26964821453311	36.0	36.0	36.0	36.0	36.0
17	35.27157933004819	36.0	36.0	36.0	36.0	36.0
18	35.27292863057689	36.0	36.0	36.0	36.0	36.0
19	35.22497637483909	36.0	36.0	36.0	36.0	36.0
20	35.20346729133234	36.0	36.0	36.0	36.0	36.0
21	35.16583463344061	36.0	36.0	36.0	36.0	36.0
22	35.13076309535494	36.0	36.0	36.0	36.0	36.0
23	35.11164894942001	36.0	36.0	36.0	36.0	36.0
24	35.09535742113519	36.0	36.0	36.0	36.0	36.0
25	35.035272090632745	36.0	36.0	36.0	36.0	36.0
26	35.00792111694304	36.0	36.0	36.0	36.0	36.0
27	34.93700098823508	36.0	36.0	36.0	36.0	36.0
28	34.870135139109415	36.0	36.0	36.0	36.0	36.0
29	34.8334531569841	36.0	36.0	36.0	36.0	36.0
30	34.772179393190356	36.0	36.0	36.0	32.0	36.0
31	34.766864396359864	36.0	36.0	36.0	32.0	36.0
32	34.72522954585478	36.0	36.0	36.0	32.0	36.0
33	34.76411902333036	36.0	36.0	36.0	36.0	36.0
34	34.72173723860403	36.0	36.0	36.0	32.0	36.0
35	34.697191520241454	36.0	36.0	36.0	32.0	36.0
36	34.61221322491685	36.0	36.0	36.0	32.0	36.0
37	34.50745534605413	36.0	36.0	36.0	32.0	36.0
38	34.46163830170969	36.0	36.0	36.0	32.0	36.0
39	34.22277100548756	36.0	36.0	36.0	32.0	36.0
40	34.304531601730986	36.0	36.0	36.0	32.0	36.0
41	34.2833708276832	36.0	36.0	36.0	32.0	36.0
42	34.25481795604356	36.0	36.0	36.0	32.0	36.0
43	34.26210658836377	36.0	36.0	36.0	32.0	36.0
44	34.157194220259846	36.0	36.0	36.0	32.0	36.0
45	34.10286007066111	36.0	36.0	36.0	32.0	36.0
46	33.99471937348235	36.0	36.0	36.0	32.0	36.0
47	33.997572463780955	36.0	36.0	36.0	32.0	36.0
48	33.97158212927986	36.0	36.0	36.0	32.0	36.0
49	33.915650645967	36.0	36.0	36.0	32.0	36.0
50	33.52175941985747	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	4.0
20	20.0
21	55.0
22	192.0
23	718.0
24	2069.0
25	4806.0
26	10594.0
27	20748.0
28	33892.0
29	49827.0
30	69196.0
31	95158.0
32	138451.0
33	211326.0
34	448110.0
35	1737036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.31149501176049	21.30375722471655	13.327996195251366	26.0567515682716
2	10.862626148089353	21.275300487583625	48.12439760743848	19.737675756888535
3	18.112928892279488	27.549418304907935	30.659953659985906	23.67769914282667
4	10.329115933437674	18.32940790455096	40.47658614901795	30.864890012993424
5	9.774070034675052	41.31805590103047	36.91457946667177	11.993294597622707
6	29.312463503546184	39.43076870744442	19.417743371110117	11.839024417899278
7	25.968365847531167	32.28981047784302	23.971946738062428	17.769876936563385
8	21.993456884568545	44.51161025624308	20.265232515166343	13.229700344022028
9	26.32386826886656	15.360553439989966	21.816502923425425	36.499075367718056
10	15.28773798341225	30.086744291604823	35.48228104073307	19.143236684249857
11	32.70629362948023	20.08349505687578	30.240808333064628	16.969402980579357
12	26.01588191919575	23.655810726584868	34.634574479582085	15.693732874637295
13	30.619094374146723	23.897536782435566	26.516731787188945	18.96663705622877
14	18.381030705445355	26.544688670517324	27.43959240352306	27.63468822051426
15	19.335249803079368	41.07826403699521	22.3269197857135	17.259566374211918
16	17.76796353770441	27.983139412721197	35.734389057059325	18.514507992515068
17	18.04207564090889	33.1211468487561	31.367410494567544	17.46936701576747
18	18.71520227283438	26.593763807918847	39.04892738048964	15.642106538757133
19	24.335882287702194	26.15293797079799	27.744992121403033	21.766187620096783
20	23.936477992546955	29.0456781457606	30.745910198522218	16.271933663170227
21	20.397115303186908	27.36982421179483	26.71041027169201	25.522650213326255
22	22.692449834402414	33.73995421307397	27.162503902093505	16.405092050430106
23	17.157624735003115	33.29802994327481	30.770182017381458	18.774163304340615
24	23.435132058182916	27.98618667757068	31.522112335647012	17.056568928599393
25	20.308496589366534	30.598861952878654	31.213807086166373	17.878834371588436
26	15.819840032768727	32.75490813382312	32.84140084891129	18.583850984496863
27	18.429361743290613	32.223408450774095	30.445435711038506	18.901794094896786
28	16.918237277757836	32.55648158548482	28.279220169491705	22.24606096726564
29	20.07360916277107	29.253494521832767	27.77794510175207	22.894951213644095
30	17.532473744801493	29.49086228028246	31.30015806800574	21.67650590691031
31	22.599685423054257	27.01963678728993	29.757427087987647	20.623250701668162
32	18.797655590331384	31.74622803533268	30.073102466406564	19.38301390792937
33	16.59986896761147	32.32832648820797	33.521436976716416	17.55036756746414
34	18.89194363410428	31.616046046297875	32.85564504041701	16.63636527918084
35	18.050367035964456	32.62773797632559	31.629971337993755	17.691923649716195
36	16.581337345329164	30.94469107998255	33.732194317701456	18.74177725698683
37	17.898110093427015	30.90688373586167	28.29282656137776	22.902179609333558
38	19.228808133220753	34.07416121377519	27.840343164542027	18.85668748846203
39	22.525665233861634	28.05322650425926	29.537669685702976	19.88343857617613
40	24.084022304561366	27.834000601657642	31.110448114469442	16.97152897931155
41	22.69305220070987	26.109709329910004	33.13578080669605	18.06145766268408
42	20.524250027372233	32.32797215508594	31.330666149812753	15.817111667729074
43	16.960615519152945	32.58691880066742	32.118561279964624	18.333904400215008
44	16.556321426913655	32.15782138988585	30.998797747716942	20.287059435483556
45	17.235329988664883	32.83973548323774	30.04741331505919	19.877521213038182
46	18.49253011570056	27.772882068202843	31.63557920614386	22.099008609952737
47	23.12913816941523	27.880817886175407	30.15957043471729	18.830473509692077
48	17.4274139741188	30.80490666334066	29.38424344386283	22.383435918677712
49	21.083635727125227	28.324007876116635	30.085256092492287	20.50710030426585
50	17.356034299482673	34.694777124229326	27.796364538303454	20.15282403798455
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	16.0
2	23.0
3	87.0
4	151.0
5	179.0
6	207.0
7	234.5
8	262.0
9	408.0
10	554.0
11	803.0
12	1052.0
13	1906.0
14	2760.0
15	3848.5
16	4937.0
17	6010.5
18	7084.0
19	8222.0
20	9360.0
21	11243.0
22	13126.0
23	16454.0
24	19782.0
25	26538.0
26	33294.0
27	50802.0
28	68310.0
29	93626.0
30	118942.0
31	153898.0
32	188854.0
33	242489.5
34	296125.0
35	350158.0
36	404191.0
37	393453.5
38	382716.0
39	335068.5
40	287421.0
41	245048.5
42	202676.0
43	174070.5
44	145465.0
45	135636.5
46	125808.0
47	120653.0
48	115498.0
49	103463.0
50	91428.0
51	76365.0
52	61302.0
53	53273.5
54	45245.0
55	44176.5
56	43108.0
57	40507.5
58	37907.0
59	33338.5
60	28770.0
61	24753.0
62	20736.0
63	18080.5
64	15425.0
65	13479.5
66	11534.0
67	10718.0
68	9902.0
69	10181.5
70	10461.0
71	8078.5
72	5696.0
73	5228.0
74	4760.0
75	3300.0
76	1840.0
77	1841.0
78	1842.0
79	1608.0
80	1374.0
81	1099.5
82	825.0
83	732.5
84	640.0
85	560.5
86	481.0
87	357.5
88	234.0
89	144.5
90	55.0
91	34.0
92	13.0
93	9.0
94	5.0
95	6.0
96	7.0
97	6.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016511923486722962
2	0.004358297401001983
3	7.086662440653631E-4
4	2.1259987321960895E-4
5	3.543331220326816E-5
6	3.897664342359497E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.4173324881307264E-4
47	3.543331220326816E-5
48	0.0
49	0.0
50	1.0629993660980448E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2822203.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.48578908450246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.76713696207499	44.551469785592424
2	11.487173727964748	12.517754498378503
3	2.9106914744035604	4.757739652932356
4	1.1726515313865735	2.5557137603499065
5	0.6171483270709122	1.681290679131963
6	0.3784511184661684	1.2372124691725022
7	0.24539811391759386	0.935949691365411
8	0.18887061683369025	0.823261167444826
9	0.1456112658612316	0.714037024803823
>10	0.8998340237927471	9.444441399886848
>50	0.09816107954472009	3.7370372426436997
>100	0.07703498207913047	8.329687357433418
>500	0.007651397031321957	2.8558198687105256
>1k	0.0037930002377325007	3.933148232854439
>5k	2.615862232918966E-4	1.1135303028993058
>10k+	1.307931116459483E-4	0.8119068663999097
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	12288	0.43540454035375914	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	10498	0.37197891150990914	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	9852	0.3490889918265979	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	9732	0.3448369943622057	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	6398	0.22670233147650967	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	5269	0.18669812199901992	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	4153	0.14715454558017266	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	3987	0.14127261575443015	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	3983	0.14113088250561706	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	3688	0.13067805540565297	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	3663	0.12979222260057127	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3589	0.1271701574975294	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	2904	0.10289833863829073	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT	2824	0.10006367366202928	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.543331220326816E-5	0.0
6	0.0	0.0	0.0	3.543331220326816E-5	0.0
7	0.0	0.0	0.0	7.086662440653632E-5	0.0
8	0.0	0.0	0.0	7.086662440653632E-5	0.0
9	0.0	0.0	0.0	7.086662440653632E-5	0.0
10	0.0	0.0	0.0	7.086662440653632E-5	0.0
11	0.0	0.0	0.0	7.086662440653632E-5	0.0
12	0.0	0.0	0.0	7.086662440653632E-5	0.0
13	0.0	0.0	0.0	7.086662440653632E-5	0.0
14	0.0	0.0	0.0	1.4173324881307264E-4	0.0
15	0.0	0.0	0.0	1.7716656101634078E-4	0.0
16	0.0	0.0	0.0	1.7716656101634078E-4	0.0
17	0.0	0.0	0.0	2.1259987321960893E-4	0.0
18	0.0	0.0	0.0	2.834664976261453E-4	0.0
19	0.0	0.0	0.0	4.6063305864248606E-4	0.0
20	0.0	0.0	0.0	6.732329318620949E-4	0.0
21	0.0	0.0	0.0	0.0010984326783013128	0.0
22	0.0	0.0	0.0	0.0015236324247405307	0.0
23	0.0	0.0	0.0	0.0023740319176189665	0.0
24	0.0	0.0	0.0	0.0037204977813431565	0.0
25	0.0	0.0	0.0	0.004535463962018324	0.0
26	0.0	0.0	0.0	0.0058464965135392455	0.0
27	0.0	0.0	0.0	0.0075827288114993854	0.0
28	0.0	0.0	0.0	0.011728426339281759	0.0
29	0.0	0.0	0.0	0.01690168992095891	0.0
30	0.0	0.0	0.0	0.027602550206345895	0.0
31	0.0	0.0	0.0	0.059173631379457824	0.0
32	0.0	0.0	0.0	0.10257943882846131	0.0
33	0.0	0.0	0.0	0.144426180540521	0.0
34	0.0	0.0	0.0	0.1927217850735755	0.0
35	0.0	0.0	0.0	0.2452339537588189	0.0
36	0.0	0.0	0.0	0.3215927415568618	0.0
37	0.0	0.0	0.0	0.4459991007025363	0.0
38	0.0	0.0	0.0	0.5962717777565966	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGCT	30	0.0057464666	29.333157	33
GGACGCG	40	7.034723E-4	27.499834	6
TAGGACC	5550	0.0	25.607054	4
TCGGACG	45	0.0013987251	24.444296	4
ACCGTCG	240	0.0	22.916529	8
TACCGTC	245	0.0	22.448845	7
GACCGTT	60	2.8742116E-4	21.999866	7
AGGACCT	10310	0.0	21.402391	5
CCGTCGT	260	0.0	21.153719	9
TGTAGGA	15420	0.0	21.001913	2
GGACCTG	10360	0.0	20.959333	6
ATACCGT	255	0.0	20.705757	6
CTGTAGG	15185	0.0	20.693478	1
AACCGGA	75	5.476208E-5	20.533209	42
TGAGCGG	65	4.934606E-4	20.30757	29
GACGTGT	175	0.0	20.114162	7
TTAGGAC	10135	0.0	20.100521	3
GTAGGAC	15765	0.0	20.011297	3
AGGACGC	55	0.0044852262	19.999878	5
CGCGAGA	55	0.0044852262	19.999878	19
>>END_MODULE