FastQCFastQC Report
Thu 2 Feb 2017
SRR4062355_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062355_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569705
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG90680.5776881643366111No Hit
TCCATGTACTCTGCGTTGATACCAC77880.4961441799573805No Hit
GTATCAACGCAGAGTACTTTTTTTT34690.22099693891527386No Hit
GAGTACATGGAAGCAGTGGTATCAA34560.22016875782392234No Hit
GTGTAAAGGTATAAGGGAGCTTGAC34270.21832127692783038No Hit
CATGTACTCTGCGTTGATACCACTG31320.19952793677792963No Hit
CCTTTACACTCTGCGAATGATTTCC30900.19685227479048611No Hit
CCCTTATACCTTTACACTCTGCGAA30750.19589668122354198No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29060.18513032703597174No Hit
TATCAACGCAGAGTACTTTTTTTTT26300.16754740540420016No Hit
GCTTCCATGTACTCTGCGTTGATAC24010.15295867694885343No Hit
CCCATGTACTCTGCGTTGATACCAC23510.14977336505903976No Hit
GCGTTGATACCACTGCTTCCATGTA22610.1440398036573751No Hit
GTATAAGGGAGCTTGACTGCGAGAG22040.1404085481029875No Hit
GTACATGGGAAGCAGTGGTATCAAC20740.1321267371894719No Hit
CATGGAAGCAGTGGTATCAACGCAG20540.13085261243354643No Hit
GTCCTAAGGTAGCGAAATTCCTTGT19980.1272850631169551No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18640.1187484272522544No Hit
GTCTCACGACGTTCTGAACCCAGCT17350.11053032257653508No Hit
GTATCAACGCAGAGTACATGGAAGC17250.10989326019857233No Hit
GTCTCAACGAGAGACTCGGTGAAAT16360.10422340503470397No Hit
GTCCCAAGGGTTGGGCTGTTCGCCC15850.100974386907094No Hit
GTCCTAAAGTGTGTATTTCTCATTT15700.10001879334014989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGT653.3735778E-614.6147965
CGGCACG851.7131242E-814.5316089
TTCGACT400.00526825714.25442819
TTATACT801.2915734E-714.2471524
TTATACC4500.014.1416194
GGGCGTA2250.013.9363316
CGGGCGT2200.013.81894715
CCTTATA4750.013.3909152
CTTATAC4850.013.3131043
ACCGTTA2700.013.0204868
TCGCGTA957.372182E-813.0019659
CCCTTAT4850.012.9165981
ACCTTTA4900.012.7978538
GTGTAAA6300.012.6556571
TGTAAAG5950.012.4453372
GTAAAGG6300.012.359063
GGACCGT2850.012.3348046
CGAAATT2700.012.31549713
AATAGGC2200.012.082712
ATAGGCC2050.012.0429143
Produced by FastQC (version 0.11.5)