##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062355_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1569705
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14152722963869	32.0	32.0	32.0	32.0	32.0
2	30.829890329711635	32.0	32.0	32.0	32.0	32.0
3	30.82419116967838	32.0	32.0	32.0	32.0	32.0
4	30.882149193638295	32.0	32.0	32.0	32.0	32.0
5	30.70162036815835	32.0	32.0	32.0	32.0	32.0
6	34.34391557649367	36.0	36.0	36.0	32.0	36.0
7	34.22457149591802	36.0	36.0	36.0	32.0	36.0
8	34.20121105558051	36.0	36.0	36.0	32.0	36.0
9	34.35331224656862	36.0	36.0	36.0	32.0	36.0
10	34.04515625547476	36.0	36.0	36.0	32.0	36.0
11	34.36689186821727	36.0	36.0	36.0	32.0	36.0
12	34.157708614038945	36.0	36.0	36.0	32.0	36.0
13	34.25325077004915	36.0	36.0	36.0	32.0	36.0
14	34.16905979148948	36.0	36.0	36.0	32.0	36.0
15	34.12010345893018	36.0	36.0	36.0	32.0	36.0
16	34.116781178629104	36.0	36.0	36.0	32.0	36.0
17	34.046850841400136	36.0	36.0	36.0	32.0	36.0
18	34.05614940386888	36.0	36.0	36.0	32.0	36.0
19	34.05066748210651	36.0	36.0	36.0	32.0	36.0
20	34.0282435234646	36.0	36.0	36.0	32.0	36.0
21	33.979531185796056	36.0	36.0	36.0	32.0	36.0
22	33.9315361803651	36.0	36.0	36.0	32.0	36.0
23	33.892405897923496	36.0	36.0	36.0	32.0	36.0
24	33.85223784086819	36.0	36.0	36.0	32.0	36.0
25	33.40685287999975	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	5.0
4	75.0
5	149.0
6	263.0
7	73.0
8	235.0
9	283.0
10	194.0
11	51.0
12	128.0
13	89.0
14	250.0
15	336.0
16	593.0
17	730.0
18	1133.0
19	1652.0
20	2386.0
21	3360.0
22	5002.0
23	7057.0
24	9666.0
25	13389.0
26	18509.0
27	23596.0
28	31780.0
29	43124.0
30	55610.0
31	75713.0
32	109591.0
33	157251.0
34	349384.0
35	658046.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.934643121319446	17.114019344188556	11.10529209648266	25.846045438009334
2	16.25035517291028	19.478574313640294	38.7181793741057	25.552891139343732
3	19.564812974317416	24.288237182917694	28.295247779085948	27.851702063678946
4	12.344460536351107	15.602211465494117	36.93296471327344	35.120363284881336
5	14.078311836378852	36.75670129504307	33.85912128768871	15.30586558088937
6	32.77204714472553	36.3483105963456	17.44217173712966	13.437470521799208
7	29.43041343746673	30.924138212413517	20.687810820786815	18.957637529332942
8	28.14110403629876	33.61282597024328	19.41130561531526	18.834764378142697
9	27.985346116689037	14.517802427647247	18.272397708178563	39.22445374748515
10	16.435304187869225	26.689135318347713	31.541050150081578	25.33451034370148
11	37.044865854024124	21.17103699626293	22.474577546853663	19.30951960285928
12	24.28521488845397	23.868400437865684	29.893327651626304	21.95305702205404
13	29.96734879763335	20.078799170548475	25.481587694215335	24.472264337602844
14	23.38562400850667	20.737112898997328	24.780587748634794	31.096675343861207
15	26.046292699820718	27.11824084934367	23.388649608104632	23.44681684273098
16	25.488222703535612	26.249799098430287	24.70483413821518	23.55714405981892
17	23.632005337582306	26.24370834399085	25.899010748518098	24.225275569908742
18	24.00976519640114	24.923003710111203	28.26063658771836	22.806594505769297
19	26.140033822252644	25.459250947463236	24.784719639552517	23.615995590731607
20	24.529064890912977	24.49666248879789	27.11373051789627	23.860542102392866
21	26.62275819615923	23.801601755488296	24.150530569544955	25.425109478807517
22	24.668154579607172	25.941494140226222	24.913736779778674	24.47661450038793
23	23.673939393939396	25.67993620414673	25.841850079744816	24.80427432216906
24	24.265078318072035	26.595610309380945	24.78581662713657	24.353494745410448
25	24.318169349930916	25.289005357172474	25.368680380041564	25.024144912855046
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	246.0
1	246.0
2	641.0
3	1036.0
4	1036.0
5	1036.0
6	2671.0
7	4306.0
8	4306.0
9	4306.0
10	4128.0
11	3950.0
12	3950.0
13	3950.0
14	3861.5
15	3773.0
16	3773.0
17	3773.0
18	6029.5
19	8286.0
20	8286.0
21	8286.0
22	14035.5
23	19785.0
24	19785.0
25	19785.0
26	30865.0
27	41945.0
28	41945.0
29	41945.0
30	54932.0
31	67919.0
32	67919.0
33	67919.0
34	81609.0
35	95299.0
36	95299.0
37	95299.0
38	109942.0
39	124585.0
40	124585.0
41	124585.0
42	158498.5
43	192412.0
44	192412.0
45	192412.0
46	219517.0
47	246622.0
48	246622.0
49	246622.0
50	237711.5
51	228801.0
52	228801.0
53	228801.0
54	202711.5
55	176622.0
56	176622.0
57	176622.0
58	155997.0
59	135372.0
60	135372.0
61	135372.0
62	116636.5
63	97901.0
64	97901.0
65	97901.0
66	80120.5
67	62340.0
68	62340.0
69	62340.0
70	45396.5
71	28453.0
72	28453.0
73	28453.0
74	21470.5
75	14488.0
76	14488.0
77	14488.0
78	11739.0
79	8990.0
80	8990.0
81	8990.0
82	6283.0
83	3576.0
84	3576.0
85	3576.0
86	2603.5
87	1631.0
88	1631.0
89	1631.0
90	1109.0
91	587.0
92	587.0
93	587.0
94	364.0
95	141.0
96	141.0
97	141.0
98	390.0
99	639.0
100	639.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008791460815885789
2	0.0029941931764248695
3	0.005160205261498179
4	0.015799146973475908
5	0.036185143068283535
6	0.04771597210940909
7	0.06854791186879063
8	0.08836055182343179
9	0.09683348145033621
10	0.1204684956727538
11	0.12091443933732772
12	0.1338468056099713
13	0.12244338904443827
14	0.12703023816577
15	0.11543570288684815
16	0.11295115961279349
17	0.12448198865391905
18	0.13359198065878622
19	0.1336556868965825
20	0.12231597656884574
21	0.13059778748236134
22	0.15346832685122364
23	0.14047225434078378
24	0.13524834284148932
25	0.13295491828082345
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1569705.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.76436121141725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.65988279845783	33.2107527962958
2	13.109132631617868	11.212073660537666
3	3.9210400051439684	5.030423133131821
4	1.6400116990805564	2.8053621076172415
5	0.8490923821883578	1.815544666688284
6	0.5082493210816782	1.3040974531316682
7	0.31814297311134926	0.9523626713305561
8	0.2457874064892324	0.8408753145858379
9	0.18517283491592126	0.7126918198997934
>10	1.1948196280479988	10.131479085335943
>50	0.17174677139459468	5.146849885989354
>100	0.17083431336460986	14.857749750397579
>500	0.018186529616764237	5.2898751635886505
>1k	0.007602565659467016	5.615312771567924
>5k	2.9813982978302024E-4	1.074549719901787
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	9068	0.5776881643366111	No Hit
TCCATGTACTCTGCGTTGATACCAC	7788	0.4961441799573805	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3469	0.22099693891527386	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3456	0.22016875782392234	No Hit
GTGTAAAGGTATAAGGGAGCTTGAC	3427	0.21832127692783038	No Hit
CATGTACTCTGCGTTGATACCACTG	3132	0.19952793677792963	No Hit
CCTTTACACTCTGCGAATGATTTCC	3090	0.19685227479048611	No Hit
CCCTTATACCTTTACACTCTGCGAA	3075	0.19589668122354198	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2906	0.18513032703597174	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2630	0.16754740540420016	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2401	0.15295867694885343	No Hit
CCCATGTACTCTGCGTTGATACCAC	2351	0.14977336505903976	No Hit
GCGTTGATACCACTGCTTCCATGTA	2261	0.1440398036573751	No Hit
GTATAAGGGAGCTTGACTGCGAGAG	2204	0.1404085481029875	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2074	0.1321267371894719	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2054	0.13085261243354643	No Hit
GTCCTAAGGTAGCGAAATTCCTTGT	1998	0.1272850631169551	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1864	0.1187484272522544	No Hit
GTCTCACGACGTTCTGAACCCAGCT	1735	0.11053032257653508	No Hit
GTATCAACGCAGAGTACATGGAAGC	1725	0.10989326019857233	No Hit
GTCTCAACGAGAGACTCGGTGAAAT	1636	0.10422340503470397	No Hit
GTCCCAAGGGTTGGGCTGTTCGCCC	1585	0.100974386907094	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1570	0.10001879334014989	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.2741247559254764E-4	0.0
11	0.0	0.0	0.0	1.2741247559254764E-4	0.0
12	0.0	0.0	0.0	1.2741247559254764E-4	6.370623779627382E-5
13	0.0	0.0	0.0	1.2741247559254764E-4	6.370623779627382E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACGT	65	3.3735778E-6	14.614796	5
CGGCACG	85	1.7131242E-8	14.531608	9
TTCGACT	40	0.005268257	14.254428	19
TTATACT	80	1.2915734E-7	14.247152	4
TTATACC	450	0.0	14.141619	4
GGGCGTA	225	0.0	13.93633	16
CGGGCGT	220	0.0	13.818947	15
CCTTATA	475	0.0	13.390915	2
CTTATAC	485	0.0	13.313104	3
ACCGTTA	270	0.0	13.020486	8
TCGCGTA	95	7.372182E-8	13.001965	9
CCCTTAT	485	0.0	12.916598	1
ACCTTTA	490	0.0	12.797853	8
GTGTAAA	630	0.0	12.655657	1
TGTAAAG	595	0.0	12.445337	2
GTAAAGG	630	0.0	12.35906	3
GGACCGT	285	0.0	12.334804	6
CGAAATT	270	0.0	12.315497	13
AATAGGC	220	0.0	12.08271	2
ATAGGCC	205	0.0	12.042914	3
>>END_MODULE