FastQCFastQC Report
Thu 2 Feb 2017
SRR4062355_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062355_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569705
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA41670.265463892897073No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC34770.22150658881764407No Hit
GTGTAAAGGTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGG31460.20041982410707745No Hit
CCCTTATACCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAAC29330.1868503954564711No Hit
CCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTGGGC29120.18551256446274939No Hit
GTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGGACGAAAGT20500.13059778748236134No Hit
GTCTCAACGAGAGACTCGGTGAAATTTTAGTACCTGTGAAGATGCAGGTT16260.10358634265674124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA1006.002665E-1126.39984744
CCTTATA6800.025.8846762
CCCTTAT7400.023.4893421
TTATACC7850.022.9807594
GTGTAAA8750.022.8827841
TTAGGAC11500.022.0005723
ACCTTTA8100.021.4566678
GGACCGT4650.021.29026
TGTAAAG9000.021.2685762
TAAAGGT9950.020.7837984
ACCGTTA4600.020.5650988
GGTATAA9550.020.2721338
GTAAAGG9550.020.0424063
GACCGTT4950.019.9998847
AGGTATA10100.019.6038487
GACGTGA7000.019.1713187
GATATAC3850.018.8594381
ACGTAGA708.1198965E-418.8588352
TTATCGT1404.083631E-918.85703312
TATACCT9000.018.8221135
Produced by FastQC (version 0.11.5)