FastQCFastQC Report
Thu 2 Feb 2017
SRR4062354_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062354_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478506
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG26770.18106115227127925No Hit
GTATCAACGCAGAGTACTTTTTTTT25600.17314775861579187No Hit
TCCATGTACTCTGCGTTGATACCAC24320.1644903706850023No Hit
TATCAACGCAGAGTACTTTTTTTTT17870.12086525181500786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAACC905.4326847E-712.6612053
CGTTATT1551.8189894E-1212.2523642
CGATAAC1302.6084308E-911.69399510
GTACCTC907.4561867E-611.61160856
GCGTTAT1800.011.6033541
ACTCTAA2000.011.40164810
CCGATAA1251.826993E-811.4016469
GCAGTTA2850.011.33458515
CGTCAAT2100.011.31154119
TAAGACT855.3309133E-511.1746784
AATTCCG1451.2187229E-911.1368995
AGCCCGT600.00587355211.0838086
ATAACGA1303.2590833E-810.9627512
CGGACAT1408.358256E-910.8561365
GTATAGT700.001499663210.849891
GGCTAAT700.001499663210.849891
GACGGTA2900.010.8119068
GCATCGC2750.010.71027414
TCTAATC803.7854904E-410.6828923
GTCCTAC2050.010.6514161
Produced by FastQC (version 0.11.5)