##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062354_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1478506
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.048550360972495	32.0	32.0	32.0	32.0	32.0
2	30.614017799048497	32.0	32.0	32.0	32.0	32.0
3	30.68080954693454	32.0	32.0	32.0	32.0	32.0
4	30.488005459565265	32.0	32.0	32.0	27.0	32.0
5	30.54153652403169	32.0	32.0	32.0	32.0	32.0
6	33.86351357383737	36.0	36.0	36.0	32.0	36.0
7	33.85611285987341	36.0	36.0	36.0	32.0	36.0
8	33.80843770671205	36.0	36.0	36.0	32.0	36.0
9	33.98421717598711	36.0	36.0	36.0	32.0	36.0
10	33.57930640795506	36.0	36.0	36.0	27.0	36.0
11	34.05576778180136	36.0	36.0	36.0	32.0	36.0
12	33.757549174639806	36.0	36.0	36.0	32.0	36.0
13	33.92814503289131	36.0	36.0	36.0	32.0	36.0
14	33.765657359523736	36.0	36.0	36.0	32.0	36.0
15	33.68249909029791	36.0	36.0	36.0	32.0	36.0
16	33.693326912437286	36.0	36.0	36.0	32.0	36.0
17	33.60150516805478	36.0	36.0	36.0	27.0	36.0
18	33.64554151285149	36.0	36.0	36.0	32.0	36.0
19	33.599493678077735	36.0	36.0	36.0	27.0	36.0
20	33.56081950293066	36.0	36.0	36.0	27.0	36.0
21	33.52168540404976	36.0	36.0	36.0	27.0	36.0
22	33.477377163163354	36.0	36.0	36.0	27.0	36.0
23	33.44042702565969	36.0	36.0	36.0	27.0	36.0
24	33.43829987839075	36.0	36.0	36.0	27.0	36.0
25	32.69402153254705	36.0	32.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	66.0
5	138.0
6	257.0
7	73.0
8	239.0
9	259.0
10	182.0
11	60.0
12	119.0
13	99.0
14	497.0
15	946.0
16	1302.0
17	1845.0
18	2114.0
19	2545.0
20	3157.0
21	3895.0
22	5022.0
23	6701.0
24	8932.0
25	12721.0
26	17228.0
27	23346.0
28	32397.0
29	45428.0
30	64085.0
31	94018.0
32	145146.0
33	213567.0
34	405859.0
35	386257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.12663412232065	17.337270942164903	11.148253542867154	26.38784139264729
2	17.134958947064405	19.783598711359208	36.19685929316849	26.8845830484079
3	19.098818549589872	23.423791477080186	28.19815791947848	29.279232053851466
4	13.041840064506701	15.034201540151635	35.13822671508277	36.78573168025889
5	15.030251443557477	35.93202999297674	33.387349740788906	15.650368822676878
6	34.82467483693633	34.58986586114331	16.557348539752077	14.02811076216828
7	30.43272269983661	30.111500512358187	20.768983987622182	18.686792800183014
8	28.56581926105653	32.15316666847196	19.491367077912937	19.789646992558573
9	27.42981058726276	14.16638287817722	18.39166061288835	40.012145921671674
10	16.09212059812836	26.236885214262063	31.37735978107543	26.29363440653415
11	37.75825033115095	20.962125542098832	21.750048081220694	19.529576045529527
12	24.5824672479175	23.445567166574893	28.4160000433466	23.555965542161005
13	29.504659771027207	19.163909358954736	25.201905352675798	26.129525517342255
14	23.81145872908485	19.18264740918624	24.48732613175622	32.51856772997269
15	25.41846989569656	26.584612550948062	22.199981174864515	25.79693637849086
16	26.19372968580715	25.39030335861322	23.199417659804983	25.216549295774648
17	24.353905686009202	25.380830289264427	25.05170515263686	25.213558872089507
18	25.399805354577726	24.28726521827175	25.77324824199424	24.53968118515629
19	25.683935763915933	25.073569700052357	24.871197995788663	24.371296540243048
20	25.676110371822613	24.055341669798764	24.934496781555485	25.334051176823134
21	26.697188678861927	24.13002106289747	24.043466776834876	25.12932348140573
22	25.896674777406258	24.079345251965815	24.513423364349237	25.51055660627869
23	24.729411573463235	23.958149893119266	24.993497749950556	26.318940783466942
24	24.84617182023522	24.899406360376165	24.65287490221709	25.601546917171525
25	25.046579339294677	24.30523808459526	24.878277400876254	25.76990517523381
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	89.0
1	89.0
2	341.0
3	593.0
4	593.0
5	593.0
6	1455.0
7	2317.0
8	2317.0
9	2317.0
10	2618.5
11	2920.0
12	2920.0
13	2920.0
14	3284.5
15	3649.0
16	3649.0
17	3649.0
18	6072.0
19	8495.0
20	8495.0
21	8495.0
22	13891.0
23	19287.0
24	19287.0
25	19287.0
26	28546.0
27	37805.0
28	37805.0
29	37805.0
30	46640.5
31	55476.0
32	55476.0
33	55476.0
34	69940.0
35	84404.0
36	84404.0
37	84404.0
38	98962.5
39	113521.0
40	113521.0
41	113521.0
42	132270.0
43	151019.0
44	151019.0
45	151019.0
46	174476.5
47	197934.0
48	197934.0
49	197934.0
50	199604.0
51	201274.0
52	201274.0
53	201274.0
54	189100.5
55	176927.0
56	176927.0
57	176927.0
58	163033.5
59	149140.0
60	149140.0
61	149140.0
62	132323.5
63	115507.0
64	115507.0
65	115507.0
66	95363.0
67	75219.0
68	75219.0
69	75219.0
70	57249.5
71	39280.0
72	39280.0
73	39280.0
74	30419.0
75	21558.0
76	21558.0
77	21558.0
78	17438.0
79	13318.0
80	13318.0
81	13318.0
82	9244.0
83	5170.0
84	5170.0
85	5170.0
86	3671.5
87	2173.0
88	2173.0
89	2173.0
90	1463.5
91	754.0
92	754.0
93	754.0
94	428.5
95	103.0
96	103.0
97	103.0
98	338.5
99	574.0
100	574.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007642850282650189
2	0.003314156317255391
3	0.004666873181441266
4	0.014203527073951679
5	0.03787607219720448
6	0.047953812835389235
7	0.06925910344631675
8	0.09266110519673239
9	0.09922178198803386
10	0.12397650060263536
11	0.12431467981868184
12	0.1376389409309127
13	0.12336777801375172
14	0.12749356444951865
15	0.11883617651872905
16	0.11538674851505507
17	0.12607321174212346
18	0.13358079033835507
19	0.1362862240667268
20	0.12587030421249557
21	0.13364842618156436
22	0.1538039074579339
23	0.141629455680261
24	0.13669203912598257
25	0.13479823551612236
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1478506.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.19225404045572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.84326004308883	41.93850717589693
2	12.6478833650761	13.455388500583638
3	3.6103090521719983	5.761214288030695
4	1.5276701898861498	3.2504088332182124
5	0.8462462674486181	2.2506873219457164
6	0.4987697447066898	1.5918412180878891
7	0.34556834598138036	1.2867091473447179
8	0.2534938172906951	1.0787126021609215
9	0.1984624813386019	0.9501000052375905
>10	1.0385860302834728	10.063464987087551
>50	0.09104429407345063	3.3987806293245946
>100	0.08713132921636704	9.775356285150108
>500	0.008903876490538792	3.1627484042045357
>1k	0.0026711629471616372	2.0360806017269675
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	2677	0.18106115227127925	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2560	0.17314775861579187	No Hit
TCCATGTACTCTGCGTTGATACCAC	2432	0.1644903706850023	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1787	0.12086525181500786	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.76358432092937E-5	0.0
3	0.0	0.0	0.0	6.76358432092937E-5	0.0
4	0.0	0.0	0.0	6.76358432092937E-5	0.0
5	0.0	0.0	0.0	6.76358432092937E-5	0.0
6	0.0	0.0	0.0	6.76358432092937E-5	6.76358432092937E-5
7	0.0	0.0	0.0	2.705433728371748E-4	6.76358432092937E-5
8	0.0	0.0	0.0	2.705433728371748E-4	6.76358432092937E-5
9	0.0	0.0	0.0	3.381792160464685E-4	6.76358432092937E-5
10	0.0	0.0	0.0	3.381792160464685E-4	6.76358432092937E-5
11	0.0	0.0	0.0	4.0581505925576226E-4	6.76358432092937E-5
12	0.0	0.0	0.0	4.7345090246505596E-4	1.352716864185874E-4
13	0.0	6.76358432092937E-5	0.0	4.7345090246505596E-4	1.352716864185874E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAACC	90	5.4326847E-7	12.661205	3
CGTTATT	155	1.8189894E-12	12.252364	2
CGATAAC	130	2.6084308E-9	11.693995	10
GTACCTC	90	7.4561867E-6	11.6116085	6
GCGTTAT	180	0.0	11.603354	1
ACTCTAA	200	0.0	11.401648	10
CCGATAA	125	1.826993E-8	11.401646	9
GCAGTTA	285	0.0	11.334585	15
CGTCAAT	210	0.0	11.311541	19
TAAGACT	85	5.3309133E-5	11.174678	4
AATTCCG	145	1.2187229E-9	11.136899	5
AGCCCGT	60	0.005873552	11.083808	6
ATAACGA	130	3.2590833E-8	10.96275	12
CGGACAT	140	8.358256E-9	10.856136	5
GTATAGT	70	0.0014996632	10.84989	1
GGCTAAT	70	0.0014996632	10.84989	1
GACGGTA	290	0.0	10.811906	8
GCATCGC	275	0.0	10.710274	14
TCTAATC	80	3.7854904E-4	10.682892	3
GTCCTAC	205	0.0	10.651416	1
>>END_MODULE