FastQCFastQC Report
Thu 2 Feb 2017
SRR4062354_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062354_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478506
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15070.10192721571640562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC602.873287E-421.99989910
CTATTAG901.001077E-519.5581111
TATACTG2000.018.6999135
TTAATCG1751.9099389E-1017.59991842
GATATAG1154.268868E-617.2196431
GGTATAT902.2135813E-417.1133481
AATAGAC902.21587E-417.1110327
TACGTAG800.001988694316.49992426
TAGGACG1753.6270649E-916.3427834
TCACGTA1958.931238E-1015.79479930
ACCGTCG3400.015.5293418
ATACCGT3700.015.4593896
TAATCGT1857.570634E-915.45938943
ATTACTC1004.944794E-415.3999293
GTATCAA30600.015.3876311
CGCAATA4300.015.34876636
CGTAAAC2455.456968E-1215.26523632
TCTAGCG4550.014.98894228
TTATACT2355.0931703E-1114.9786544
CTAATTG2651.8189894E-1214.94332840
Produced by FastQC (version 0.11.5)