##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062354_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1478506
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.137279794603472	32.0	32.0	32.0	32.0	32.0
2	31.018865665746368	32.0	32.0	32.0	32.0	32.0
3	31.26165467032261	32.0	32.0	32.0	32.0	32.0
4	31.471333900572606	32.0	32.0	32.0	32.0	32.0
5	31.303399512751387	32.0	32.0	32.0	32.0	32.0
6	34.926949907541804	36.0	36.0	36.0	36.0	36.0
7	34.946688752024	36.0	36.0	36.0	36.0	36.0
8	34.86583010146729	36.0	36.0	36.0	32.0	36.0
9	35.075223908458945	36.0	36.0	36.0	36.0	36.0
10	34.8624435747978	36.0	36.0	36.0	32.0	36.0
11	35.0806307177651	36.0	36.0	36.0	36.0	36.0
12	34.952380984588494	36.0	36.0	36.0	32.0	36.0
13	35.02976788731328	36.0	36.0	36.0	36.0	36.0
14	34.97774848394258	36.0	36.0	36.0	32.0	36.0
15	34.90714816172542	36.0	36.0	36.0	32.0	36.0
16	34.94120348513973	36.0	36.0	36.0	32.0	36.0
17	34.90234466414069	36.0	36.0	36.0	32.0	36.0
18	34.917737229338265	36.0	36.0	36.0	32.0	36.0
19	34.90651914838357	36.0	36.0	36.0	32.0	36.0
20	34.905953712734345	36.0	36.0	36.0	32.0	36.0
21	34.9015986407901	36.0	36.0	36.0	32.0	36.0
22	34.87229270628594	36.0	36.0	36.0	32.0	36.0
23	34.828858996852226	36.0	36.0	36.0	32.0	36.0
24	34.80688411139353	36.0	36.0	36.0	32.0	36.0
25	34.78382569972661	36.0	36.0	36.0	32.0	36.0
26	34.72266463578775	36.0	36.0	36.0	32.0	36.0
27	34.69846723652119	36.0	36.0	36.0	32.0	36.0
28	34.68770705022502	36.0	36.0	36.0	32.0	36.0
29	34.65270685408108	36.0	36.0	36.0	32.0	36.0
30	34.64990537745535	36.0	36.0	36.0	32.0	36.0
31	34.65091044608544	36.0	36.0	36.0	32.0	36.0
32	34.6013665145762	36.0	36.0	36.0	32.0	36.0
33	34.58713052229751	36.0	36.0	36.0	32.0	36.0
34	34.568805943296816	36.0	36.0	36.0	32.0	36.0
35	34.548154691289724	36.0	36.0	36.0	32.0	36.0
36	34.504319224947345	36.0	36.0	36.0	32.0	36.0
37	34.498777143954776	36.0	36.0	36.0	32.0	36.0
38	34.45786828054807	36.0	36.0	36.0	32.0	36.0
39	34.44453252134249	36.0	36.0	36.0	32.0	36.0
40	34.43911962481045	36.0	36.0	36.0	32.0	36.0
41	34.39517188296835	36.0	36.0	36.0	32.0	36.0
42	34.34660596575191	36.0	36.0	36.0	32.0	36.0
43	34.35642127931845	36.0	36.0	36.0	32.0	36.0
44	34.31849177480511	36.0	36.0	36.0	32.0	36.0
45	34.26811727514126	36.0	36.0	36.0	32.0	36.0
46	34.28811043039393	36.0	36.0	36.0	32.0	36.0
47	34.25634525663068	36.0	36.0	36.0	32.0	36.0
48	34.219993696339415	36.0	36.0	36.0	32.0	36.0
49	34.2058226344702	36.0	36.0	36.0	32.0	36.0
50	33.58935709425596	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	6.0
21	31.0
22	112.0
23	326.0
24	900.0
25	2140.0
26	4477.0
27	8771.0
28	15193.0
29	24330.0
30	36559.0
31	56474.0
32	89223.0
33	154068.0
34	331197.0
35	754698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.55811579664392	17.990939795025884	11.753102988298306	26.697841420031885
2	16.69581897700417	20.198560376396234	35.685133403948214	27.420487242651387
3	18.69772978603242	23.556792549180077	28.71325232753577	29.03222533725173
4	13.015165308764388	15.57673759862997	35.5299200679605	35.87817702464515
5	15.186150875377495	35.72656162813112	33.14767281815077	15.939614678340622
6	35.145660748973285	34.961691006585035	16.096067376212712	13.796580868228967
7	30.56720770832178	30.131768149740346	20.571982798852357	18.72904134308552
8	28.597313774851102	32.392428573167784	19.176925896817462	19.83333175516366
9	27.61943475373113	13.880701194313719	18.132966656882015	40.36689739507314
10	16.080083543793535	26.29296059671046	31.297066092393266	26.32988976710274
11	38.11144493157282	20.851048287933903	21.606067205679246	19.431439574814036
12	24.7074411601982	23.361555516176466	28.231268591402404	23.699734732222932
13	29.771877828023698	19.052814124528407	25.10980679145029	26.06550125599761
14	23.96507014513299	19.00005816682516	24.364865614343127	32.67000607369872
15	25.65150225971352	26.57398752524508	21.97231529665757	25.80219491838383
16	26.44946993789677	25.32887928760519	23.07011266778762	25.151538106710426
17	24.565135346085846	25.371625140513466	24.82431589726386	25.238923616136834
18	25.61065021041511	24.32144340300276	25.54456999159963	24.523336394982504
19	25.882816843489305	24.995637488113	24.702165564427876	24.419380103969818
20	25.872536195321494	23.935107466591273	24.74355870047196	25.448797637615268
21	26.924476464755635	24.076195835525862	23.89472886819533	25.104598831523173
22	25.912103163598932	24.111095930621858	24.465710656568184	25.511090249211026
23	24.9315863445938	23.756819383891578	24.92272604913338	26.38886822238124
24	25.045958555460718	24.87206680256962	24.495267520050646	25.586707121919016
25	25.410312842829182	24.03304416755833	24.726108652923966	25.830534336688523
26	25.066722759325966	24.605716852011422	25.065099499088944	25.262460889573664
27	25.870033669122748	24.48748939808158	24.35979292610243	25.282684006693245
28	25.106492635133034	23.933010755451786	25.190022901496512	25.77047370791867
29	24.972303122205794	24.519548787762783	25.139566562462377	25.368581527569045
30	25.1557315289894	24.45759435538307	25.255156218507064	25.13151789712047
31	25.308453262955982	24.367503412228288	24.329356796658246	25.99468652815748
32	25.201994445744557	24.461923049348464	24.31001294550039	26.02606955940659
33	24.824789348166323	24.157494119063433	24.87598968147576	26.141726851294482
34	25.416535340404433	24.115695167960087	25.34774968786058	25.12001980377489
35	26.211256498113638	24.04690951541624	24.74883429624229	24.992999690227837
36	25.10777771615401	24.618161847161932	24.638790779340766	25.63526965734329
37	26.030127709999146	24.3246222876336	24.502166376057993	25.14308362630926
38	25.132126619709354	24.120091497768694	24.903314562132316	25.844467320389636
39	25.558976426203206	24.21214388037654	24.41166961784396	25.81721007557629
40	25.80009820724434	24.208356273156824	24.859283628203062	25.132261891395775
41	24.776903171174144	24.542274431081104	25.285051261205567	25.39577113653918
42	25.975004497783573	24.733683867363407	24.660298977481325	24.6310126573717
43	25.19969482707544	23.70101981324391	24.97467037671812	26.12461498296253
44	24.96865078667249	24.230337922199844	25.02607361755718	25.77493767357048
45	25.124280861897073	24.532737777188597	24.83216165507614	25.510819705838188
46	24.956374822117493	24.159894055071884	25.185660640755792	25.698070482054835
47	25.13354697241675	23.999429153483316	25.4344588388549	25.432565035245037
48	25.558638246987158	24.942746258723332	24.403620952502052	25.09499454178745
49	24.66699492595904	24.68045445875769	25.05319559068411	25.599355024599156
50	24.560214541039766	25.029337066834405	24.633531844667417	25.776916547458413
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	12.0
2	14.0
3	45.5
4	77.0
5	109.0
6	141.0
7	152.5
8	164.0
9	216.0
10	268.0
11	367.0
12	466.0
13	852.5
14	1239.0
15	1655.0
16	2071.0
17	2540.5
18	3010.0
19	3230.5
20	3451.0
21	4231.0
22	5011.0
23	5357.0
24	5703.0
25	7020.0
26	8337.0
27	10601.5
28	12866.0
29	14551.5
30	16237.0
31	17631.0
32	19025.0
33	22008.0
34	24991.0
35	29519.5
36	34048.0
37	41781.0
38	49514.0
39	54415.0
40	59316.0
41	66272.0
42	73228.0
43	76085.0
44	78942.0
45	87772.5
46	96603.0
47	103490.5
48	110378.0
49	113057.5
50	115737.0
51	112783.0
52	109829.0
53	108621.0
54	107413.0
55	108904.5
56	110396.0
57	106627.0
58	102858.0
59	94953.0
60	87048.0
61	77419.5
62	67791.0
63	59657.0
64	51523.0
65	44233.5
66	36944.0
67	31630.5
68	26317.0
69	23572.5
70	20828.0
71	16472.5
72	12117.0
73	10722.5
74	9328.0
75	7217.0
76	5106.0
77	4456.0
78	3806.0
79	3215.5
80	2625.0
81	2104.0
82	1583.0
83	1305.5
84	1028.0
85	834.0
86	640.0
87	470.5
88	301.0
89	204.5
90	108.0
91	73.5
92	39.0
93	26.5
94	14.0
95	11.5
96	9.0
97	6.5
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.011701000875207812
2	0.004328693965394797
3	7.439942753022308E-4
4	0.0
5	6.76358432092937E-5
6	1.352716864185874E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.352716864185874E-4
47	0.0
48	0.0
49	0.0
50	6.76358432092937E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1478506.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.153045270145164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.34826315242145	45.9214792132788
2	11.957698617676282	13.668377808456098
3	3.3613796016662785	5.76339241632526
4	1.35154872426852	3.089805016917027
5	0.7405561998791754	2.1162521008390596
6	0.46182709905062413	1.5836895059412084
7	0.3227872605209561	1.2913792439226242
8	0.21974468332141145	1.0047262266993875
9	0.17247094710771727	0.887151586304896
>10	0.90747248811607	9.410586834148555
>50	0.07634976733435289	3.050926946702214
>100	0.07160316324508598	8.535060170730844
>500	0.006520089236690621	2.4489417490324907
>1k	0.0017782061554610783	1.2282311807016457
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1507	0.10192721571640562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.76358432092937E-5	0.0
5	0.0	0.0	0.0	6.76358432092937E-5	0.0
6	0.0	0.0	0.0	6.76358432092937E-5	0.0
7	0.0	0.0	0.0	2.0290752962788113E-4	0.0
8	0.0	0.0	0.0	2.0290752962788113E-4	1.352716864185874E-4
9	0.0	0.0	0.0	2.705433728371748E-4	1.352716864185874E-4
10	0.0	0.0	0.0	2.705433728371748E-4	1.352716864185874E-4
11	0.0	0.0	0.0	2.705433728371748E-4	1.352716864185874E-4
12	0.0	0.0	0.0	3.381792160464685E-4	1.352716864185874E-4
13	0.0	0.0	0.0	3.381792160464685E-4	1.352716864185874E-4
14	0.0	0.0	0.0	3.381792160464685E-4	2.0290752962788113E-4
15	0.0	0.0	0.0	3.381792160464685E-4	2.0290752962788113E-4
16	0.0	0.0	0.0	3.381792160464685E-4	2.0290752962788113E-4
17	0.0	0.0	0.0	3.381792160464685E-4	2.0290752962788113E-4
18	0.0	0.0	0.0	4.7345090246505596E-4	2.0290752962788113E-4
19	0.0	0.0	0.0	4.7345090246505596E-4	2.0290752962788113E-4
20	0.0	0.0	0.0	6.76358432092937E-4	2.705433728371748E-4
21	0.0	0.0	0.0	7.439942753022308E-4	2.705433728371748E-4
22	0.0	0.0	0.0	0.0010145376481394057	2.705433728371748E-4
23	0.0	0.0	0.0	0.0012850810209765805	3.381792160464685E-4
24	0.0	0.0	0.0	0.0019614394530695174	4.0581505925576226E-4
25	0.0	0.0	0.0	0.002502526198743867	4.0581505925576226E-4
26	0.0	0.0	0.0	0.0028407054147903356	4.0581505925576226E-4
27	0.0	0.0	0.0	0.004125786435766916	4.0581505925576226E-4
28	0.0	0.0	0.0	0.008251572871533832	4.0581505925576226E-4
29	0.0	0.0	0.0	0.01772059092083495	4.0581505925576226E-4
30	0.0	0.0	0.0	0.03402082913427473	4.0581505925576226E-4
31	0.0	0.0	0.0	0.07805176306352493	4.0581505925576226E-4
32	0.0	0.0	0.0	0.12864337378407664	4.7345090246505596E-4
33	0.0	0.0	0.0	0.17842335438611678	4.7345090246505596E-4
34	0.0	0.0	0.0	0.2352374626819235	4.7345090246505596E-4
35	0.0	0.0	0.0	0.3029409417344265	4.7345090246505596E-4
36	0.0	0.0	0.0	0.3940464225373451	4.7345090246505596E-4
37	0.0	0.0	0.0	0.5326999011163972	4.7345090246505596E-4
38	0.0	0.0	0.0	0.7124759723666999	4.7345090246505596E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGC	60	2.873287E-4	21.999899	10
CTATTAG	90	1.001077E-5	19.558111	1
TATACTG	200	0.0	18.699913	5
TTAATCG	175	1.9099389E-10	17.599918	42
GATATAG	115	4.268868E-6	17.219643	1
GGTATAT	90	2.2135813E-4	17.113348	1
AATAGAC	90	2.21587E-4	17.111032	7
TACGTAG	80	0.0019886943	16.499924	26
TAGGACG	175	3.6270649E-9	16.342783	4
TCACGTA	195	8.931238E-10	15.794799	30
ACCGTCG	340	0.0	15.529341	8
ATACCGT	370	0.0	15.459389	6
TAATCGT	185	7.570634E-9	15.459389	43
ATTACTC	100	4.944794E-4	15.399929	3
GTATCAA	3060	0.0	15.387631	1
CGCAATA	430	0.0	15.348766	36
CGTAAAC	245	5.456968E-12	15.265236	32
TCTAGCG	455	0.0	14.988942	28
TTATACT	235	5.0931703E-11	14.978654	4
CTAATTG	265	1.8189894E-12	14.943328	40
>>END_MODULE