FastQCFastQC Report
Thu 2 Feb 2017
SRR4062353_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062353_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562250
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG33950.21731477036325814No Hit
GTATCAACGCAGAGTACTTTTTTTT30380.19446311409825573No Hit
TCCATGTACTCTGCGTTGATACCAC27820.1780764922387582No Hit
TATCAACGCAGAGTACTTTTTTTTT20030.12821251400224037No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19750.12642022723635782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA2100.012.66758610
AAGACGG2100.012.2124325
CGTCTAT907.5185617E-611.6030281
TCGCGTA752.072428E-411.4008269
CAAGACG2500.011.3968094
CCTAGTG1104.998037E-711.2219722
GGTTAAG1105.012553E-711.2194571
CGGCTAA1201.273711E-711.0841379
CTACCGA600.00588793511.080234
ATCGCCA1553.110472E-1011.03447316
CTAAACG1303.2569005E-810.96338812
CTAGACA1303.2785465E-810.958474
TTACGAC700.001490505410.85793111
TATATCT1951.8189894E-1210.7149494
CGCATCG1605.4205884E-1010.68861813
CGCGGTC803.7681655E-410.68827510
GCCGGTC2950.010.6282318
TCCCTAA1701.382432E-1010.61943812
TTGCGCG1355.6630597E-810.558351518
CGAACGA909.494861E-510.55767516
Produced by FastQC (version 0.11.5)