##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062353_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1562250
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.139005280844934	32.0	32.0	32.0	32.0	32.0
2	30.766822211553848	32.0	32.0	32.0	32.0	32.0
3	30.762043847015523	32.0	32.0	32.0	32.0	32.0
4	30.835844455112817	32.0	32.0	32.0	32.0	32.0
5	30.611127540406464	32.0	32.0	32.0	32.0	32.0
6	34.259325972155544	36.0	36.0	36.0	32.0	36.0
7	34.146939350296044	36.0	36.0	36.0	32.0	36.0
8	34.134392062730036	36.0	36.0	36.0	32.0	36.0
9	34.31308241318611	36.0	36.0	36.0	32.0	36.0
10	33.96277484397503	36.0	36.0	36.0	32.0	36.0
11	34.29902000320051	36.0	36.0	36.0	32.0	36.0
12	34.06921555448872	36.0	36.0	36.0	32.0	36.0
13	34.19378652584413	36.0	36.0	36.0	32.0	36.0
14	34.095786205792926	36.0	36.0	36.0	32.0	36.0
15	34.034260201632264	36.0	36.0	36.0	32.0	36.0
16	34.033930548887824	36.0	36.0	36.0	32.0	36.0
17	33.95447143542967	36.0	36.0	36.0	32.0	36.0
18	33.970733877420386	36.0	36.0	36.0	32.0	36.0
19	33.965614978396545	36.0	36.0	36.0	32.0	36.0
20	33.96138390142423	36.0	36.0	36.0	32.0	36.0
21	33.939290126420225	36.0	36.0	36.0	32.0	36.0
22	33.89188158105297	36.0	36.0	36.0	32.0	36.0
23	33.83971515442471	36.0	36.0	36.0	32.0	36.0
24	33.808378300528084	36.0	36.0	36.0	32.0	36.0
25	33.33852648423748	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	59.0
5	120.0
6	297.0
7	62.0
8	235.0
9	279.0
10	180.0
11	58.0
12	96.0
13	83.0
14	249.0
15	320.0
16	485.0
17	733.0
18	988.0
19	1394.0
20	2158.0
21	3183.0
22	4867.0
23	7336.0
24	10041.0
25	14330.0
26	19606.0
27	25521.0
28	34389.0
29	45155.0
30	58711.0
31	79550.0
32	112685.0
33	159587.0
34	346086.0
35	633398.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.49660365618284	17.380471807241566	11.164884154300466	25.958040382275126
2	16.65063157766712	20.024977611687113	37.107756438200255	26.216634372445508
3	19.00713550938524	24.00077583541535	28.422589613151306	28.5694990420481
4	12.568308109907555	15.424316918900924	36.226473073672885	35.78090189751863
5	14.312992129765698	36.695807856403285	34.049280690936264	14.941919322894757
6	33.92862631986106	35.41838506898372	17.064119247301363	13.588869363853867
7	29.76221663823788	30.66958918429347	21.19815963377148	18.37003454369717
8	27.82977829201695	33.136969995169395	19.85986128355635	19.173390429257303
9	27.052758774014897	14.645419470246706	18.701667476079606	39.60015427965879
10	15.287883429095817	27.31313498985554	32.58568958600616	24.81329199504248
11	36.83084980120635	21.469009466730583	22.382934763383002	19.317205968680067
12	23.931187333198313	24.025852499237292	29.40478235343884	22.638177814125555
13	29.185769747897005	20.008151787305	25.455301673808023	25.350776790989972
14	22.926629811309628	19.738978962196555	25.794477336262624	31.53991389023119
15	24.87415884278042	27.238274423758043	22.951312368671818	24.936254364789725
16	25.314731201675322	26.202758279516424	24.10675036429744	24.375760154510818
17	23.64860188261351	26.11434108527132	25.823194803330463	24.413862228784712
18	24.326557191284103	25.37614670666934	26.604043380811405	23.693252721235154
19	24.775612019658464	25.937609758473673	25.790196917917967	23.496581303949895
20	24.750351513784338	24.994905132934374	26.06610894427946	24.18863440900183
21	25.61935904814766	24.97170310759485	24.993750969400903	24.41518687485659
22	24.716610415044023	25.246689808359857	25.371116474513688	24.66558330208243
23	23.954183011563213	24.98295002948491	25.785580596364383	25.277286362587493
24	23.891433168070915	25.93579442936394	25.553842751795507	24.618929650769637
25	24.381445842902018	25.225690627742015	25.61518922761088	24.777674301745083
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	247.0
1	247.0
2	566.5
3	886.0
4	886.0
5	886.0
6	2369.0
7	3852.0
8	3852.0
9	3852.0
10	4160.0
11	4468.0
12	4468.0
13	4468.0
14	5179.5
15	5891.0
16	5891.0
17	5891.0
18	9429.5
19	12968.0
20	12968.0
21	12968.0
22	19762.0
23	26556.0
24	26556.0
25	26556.0
26	37802.5
27	49049.0
28	49049.0
29	49049.0
30	60319.5
31	71590.0
32	71590.0
33	71590.0
34	86609.5
35	101629.0
36	101629.0
37	101629.0
38	116171.0
39	130713.0
40	130713.0
41	130713.0
42	147612.0
43	164511.0
44	164511.0
45	164511.0
46	188398.5
47	212286.0
48	212286.0
49	212286.0
50	210458.0
51	208630.0
52	208630.0
53	208630.0
54	194217.5
55	179805.0
56	179805.0
57	179805.0
58	162302.5
59	144800.0
60	144800.0
61	144800.0
62	126048.5
63	107297.0
64	107297.0
65	107297.0
66	87370.5
67	67444.0
68	67444.0
69	67444.0
70	50776.5
71	34109.0
72	34109.0
73	34109.0
74	26025.5
75	17942.0
76	17942.0
77	17942.0
78	14059.5
79	10177.0
80	10177.0
81	10177.0
82	7094.0
83	4011.0
84	4011.0
85	4011.0
86	2960.0
87	1909.0
88	1909.0
89	1909.0
90	1274.0
91	639.0
92	639.0
93	639.0
94	397.5
95	156.0
96	156.0
97	156.0
98	420.5
99	685.0
100	685.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008257321171387423
2	0.0032645223235717715
3	0.004032645223235718
4	0.01344215074411906
5	0.03475756120979357
6	0.046215394463114096
7	0.06650664106257001
8	0.08756601056168987
9	0.09268682989278285
10	0.11470635301648263
11	0.1176508241318611
12	0.12891662666026565
13	0.11880300848135701
14	0.12264362297967676
15	0.1120819331092975
16	0.10958553368538965
17	0.11944311089774363
18	0.12936469835173628
19	0.12840454472715634
20	0.11880300848135701
21	0.12853256521043366
22	0.1469035045607297
23	0.13634181469035045
24	0.1347415586493839
25	0.13051688270123218
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1562250.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.44001365244329
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.62066007835118	48.51533149378627
2	11.340550073209785	13.481649023556127
3	3.079133376634801	5.4907118983459915
4	1.2798845815664484	3.043054280074454
5	0.6660610806136592	1.979533986251852
6	0.425520632407301	1.5175771319811762
7	0.27838655831988457	1.1583110579033444
8	0.19320993407178824	0.9187520895211808
9	0.14945457176521248	0.7995223607529865
>10	0.8219739238153931	8.92828148422445
>50	0.07405259547530817	3.0973266975529734
>100	0.06528557883791429	7.876336725046815
>500	0.004532122724338292	1.8149792589835714
>1k	0.0012948922069537976	1.3786325120186904
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3395	0.21731477036325814	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3038	0.19446311409825573	No Hit
TCCATGTACTCTGCGTTGATACCAC	2782	0.1780764922387582	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2003	0.12821251400224037	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1975	0.12642022723635782	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9203072491598655E-4
2	0.0	0.0	0.0	0.0	1.9203072491598655E-4
3	0.0	0.0	0.0	0.0	1.9203072491598655E-4
4	0.0	0.0	0.0	0.0	1.9203072491598655E-4
5	0.0	0.0	0.0	6.401024163866218E-5	1.9203072491598655E-4
6	0.0	0.0	0.0	6.401024163866218E-5	3.200512081933109E-4
7	0.0	0.0	0.0	1.2802048327732437E-4	3.200512081933109E-4
8	0.0	0.0	0.0	1.2802048327732437E-4	3.840614498319731E-4
9	0.0	0.0	0.0	2.5604096655464873E-4	3.840614498319731E-4
10	0.0	0.0	0.0	2.5604096655464873E-4	3.840614498319731E-4
11	0.0	0.0	0.0	3.840614498319731E-4	3.840614498319731E-4
12	0.0	0.0	0.0	4.480716914706353E-4	5.760921747479596E-4
13	0.0	0.0	0.0	4.480716914706353E-4	6.401024163866218E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCCA	210	0.0	12.667586	10
AAGACGG	210	0.0	12.212432	5
CGTCTAT	90	7.5185617E-6	11.603028	1
TCGCGTA	75	2.072428E-4	11.400826	9
CAAGACG	250	0.0	11.396809	4
CCTAGTG	110	4.998037E-7	11.221972	2
GGTTAAG	110	5.012553E-7	11.219457	1
CGGCTAA	120	1.273711E-7	11.084137	9
CTACCGA	60	0.005887935	11.08023	4
ATCGCCA	155	3.110472E-10	11.034473	16
CTAAACG	130	3.2569005E-8	10.963388	12
CTAGACA	130	3.2785465E-8	10.95847	4
TTACGAC	70	0.0014905054	10.857931	11
TATATCT	195	1.8189894E-12	10.714949	4
CGCATCG	160	5.4205884E-10	10.688618	13
CGCGGTC	80	3.7681655E-4	10.688275	10
GCCGGTC	295	0.0	10.628231	8
TCCCTAA	170	1.382432E-10	10.619438	12
TTGCGCG	135	5.6630597E-8	10.5583515	18
CGAACGA	90	9.494861E-5	10.557675	16
>>END_MODULE